FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961216
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29940.3114804580864239No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26760.2783973633397696No Hit
GAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT22600.23511884945735403No Hit
GTATTATTGTGCAAAAGATAGGGGTGTATTACCGAAGTGGGGACTTGACTACTGGGGCCAGGGAGCCCTGGTCAC22060.22950096544377122No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGCATTAGTTGGGATAGTGGT20400.21223117384646115No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20100.2091101271722485No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18860.19620980091883614No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17410.18112474199347492No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16970.17654720687129635No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16570.17238581130567948No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA16300.16957686929888807No Hit
GTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATCCAGGGAAAGTCCCTAAGCTCCTGATCTATGGTGCATC15860.1649993341767095No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15640.16271056661562022No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15590.1621903921699181No Hit
CAATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATA15480.16104600838937347No Hit
GTCTGAGACCTGAGGACACGGCCCTGTATTATTGTGCAAAAGATAGGGGT14940.15542812437579068No Hit
ACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA14920.15522005459750982No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14880.15480391504094812No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14860.1545958452626673No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14510.1509546241427525No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14380.14960217058392702No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14290.14866585658166323No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT14240.1481456821359611No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAG14000.145648844796591No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13760.14315200745722087No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13660.14211165856581665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13410.13951078633730607No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13040.13566149543911046No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12980.13503728610426793No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12730.13243641387575739No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12610.13118799520607233No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12600.13108396031693187No Hit
CCATAGATCAGGAGCTTAGGGACTTTCCCTGGATTCTGCTGATACCAATT12500.13004361142552764No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12440.12941940209068514No Hit
AGTCAGTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATCCAGGGAA12170.12661046008389373No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCAAAATCATCTACGGTGAAC12130.12619432052733204No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC12120.12609028563819163No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGT12110.1259862507490512No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12100.12588221585991077No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12060.12546607630334908No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11690.12161678540515347No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGACCCAGTC11580.12047240162460883No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11530.11995222717890672No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11510.11974415740062588No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA11340.1179755642852387No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGTA11320.11776749450695785No Hit
CTCCTGTGCAGCCTCTGGGTTCACCGTAGATGATTTTGCCATGCACTGGGTCCGGCAAGGTCCAGGGAAGGGC11130.11579083161328983No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11060.11506258738930687No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11060.11506258738930687No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG10940.11381416871962181No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAGCTATTTAAATTGGTATCAGCAGAAT10820.11256575004993674No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10790.11225364538251549No Hit
GTGCCAGATGTGTCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA10760.11194154071509423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10620.1104850522671283No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10550.10975680804314536No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10520.10944470337572408No Hit
GTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA10470.10892452893002198No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10390.10809224981689859No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10380.10798821492775817No Hit
ATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACA10380.10798821492775817No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10340.10757207537119648No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCAT10320.10736400559291563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10110.10517927292096677No Hit
TCCATAGACTATGCGGACTCTGTGAAGGGCCGCTTCACCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG10090.10497120314268593No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10030.10434699380784339No Hit
GTCTATGGAACCACTATCCCAACTAATGCCTGAGACCCACTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTG9930.10330664491643916No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9920.10320261002729875No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA9660.10049770290964778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)