FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961216
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28200.2933783873759904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25090.26102353685331914No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23950.249163559491311No Hit
GAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT21930.22814851188494573No Hit
GTATTATTGTGCAAAAGATAGGGGTGTATTACCGAAGTGGGGACTTGACTACTGGGGCCAGGGAGCCCTGGTCAC21390.22253062787136293No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGCATTAGTTGGGATAGTGGT19460.20245189426726148No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19100.19870663825820628No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17250.1794601837672282No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16520.17186563685997736No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15450.16073390372195218No Hit
GTCTGAGACCTGAGGACACGGCCCTGTATTATTGTGCAAAAGATAGGGGT15340.15958951994140755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15210.15823706638258206No Hit
CAATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATA15190.15802899660430123No Hit
GTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATCCAGGGAAAGTCCCTAAGCTCCTGATCTATGGTGCATC15130.15740478726945867No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14810.15407567081696516No Hit
ACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA14310.14887392635994406No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14280.1485618216925228No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14110.14679322857713564No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT13820.14377621679206337No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAG13370.1390946467807444No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13170.13701394899793595No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12950.13472518143684667No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12610.13118799520607233No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12590.13097992542779147No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12520.1302516812038085No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12520.1302516812038085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12300.12796291364271922No Hit
AGTCAGTACATTAGCAGCTATTTAAATTGGTATCAGCAGAATCCAGGGAA12270.12765080897529796No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12080.12567414608162994No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12060.12546607630334908No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12010.12494590185764698No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11980.12463379719022571No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGT11800.12276116918569811No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11750.122240994739996No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11740.12213695985085558No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11690.12161678540515347No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAGCTATTTAAATTGGTATCAGCAGAAT11650.12120064584859179No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11500.11964012251148544No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11450.11911994806578334No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCAAAATCATCTACGGTGAAC11440.11901591317664292No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11410.11870380850922166No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11260.11714328517211532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11250.11703925028297489No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11070.11516662227844729No Hit
CTCCTGTGCAGCCTCTGGGTTCACCGTAGATGATTTTGCCATGCACTGGGTCCGGCAAGGTCCAGGGAAGGGC11010.11454241294360477No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC10960.11402223849790265No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA10950.11391820360876223No Hit
CCATAGATCAGGAGCTTAGGGACTTTCCCTGGATTCTGCTGATACCAATT10880.11318995938477928No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGTCATCCAGATGACCCAGTC10610.11038101737798788No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG10350.1076761102603369No Hit
ATAATACAGGGCCGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACA10310.10725997070377521No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGGTA10280.10694786603635395No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10160.10569944736666889No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10130.10538734269924761No Hit
GTAGTAAGTTGCAGAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA10120.1052833078101072No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10110.10517927292096677No Hit
GTGCCAGATGTGTCATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA9840.10237033091417538No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9750.10143401691191158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9670.1006017377987882No Hit
TCCATAGACTATGCGGACTCTGTGAAGGGCCGCTTCACCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG9660.10049770290964778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)