FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639181
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26600.41615755161683465No Hit
GTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23080.3610870786209227No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22670.35467262011855794No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA19410.3036698525143895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17690.27676041684593256No Hit
CTACCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTC16580.2593944438273353No Hit
GTCTGGGACAGACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATT15720.2459397259931068No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14910.23326725919575209No Hit
CATCTATGGTGCCTCCACCAGGGCCACTGGCATCCCAGACAGGTTAAGTGGCAGTGGGTCTGGGACAGACTTCA14740.23060760567038133No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14500.22685280069338734No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14030.21949964094677407No Hit
GGTTAAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATAAGCAGA13480.21089487954116282No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13300.20807877580841733No Hit
CTACTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTACC13130.20541912228304657No Hit
GTCCCAGACCCACTGCCACTTAACCTGTCTGGGATGCCAGTGGCCCTGGT13010.20354171979454957No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12820.20056916585442933No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTACTGCAGGGCCAGTCAGACTGTTAGCAG12650.19790951232905857No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12230.1913386036193191No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12140.18993055175294635No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTACTGCAGGGCCAGTCAGA11980.18742734843495035No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTACTGC11840.18523704553170386No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11620.18179514096945934No Hit
TTGCTAGACAGTCTCGCACAATAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTTAGTTCCATGTACGCTGTG11480.17960483806621286No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTCGCTGCTGCTAACAGTCTGA11370.1778838857850906No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11110.1738161803933471No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11000.17209522811222489No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10970.1716258774901006No Hit
GTCTAGCAATGGCCGATATTACTTCGAGTTCTGGGGCCGTGGCACCCTAGTCACTGTCTCTTCAGCATCCCCGAC10770.16849687334260563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10430.1631775662918641No Hit
GCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTTAACCTGT10340.16176951442549137No Hit
CTCTACTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTA9870.15441635467887813No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC9800.15332120322725487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9680.15144380073875788No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9660.15113090032400836No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9610.15034864928713462No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9430.14753254555438913No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9270.14502934223639313No Hit
ACCCTCTACTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT8920.13955358497827688No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGA8850.13845843352665366No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTTAACCTGTCTGGGATGCCA8830.13814553311190414No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC8760.13705038166028088No Hit
GACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGGTAGTTCA8730.13658103103815664No Hit
GTTTATTACTGTCAGCAGTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8720.1364245808307819No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8610.13470362854965964No Hit
CACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGGTAGTTCACCTCTCACTTT8570.13407782772016064No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8200.1282891700472949No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8160.1276633692177959No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8160.1276633692177959No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCACCGGAGACATTGTGTTGACGCAGTCTC8000.1251601658997999No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7770.12156181113018065No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGGCACCTTCAGCAG7630.11937150822693417No Hit
ATCCCAGACAGGTTAAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC7590.11874570739743516No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7540.11796345636056141No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7370.11530380283519066No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7350.11499090242044116No Hit
GCAGTAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAG7280.11389575096881792No Hit
CTCCCAGGCTCCTCATCTATGGTGCCTCCACCAGGGCCACTGGCATCCCA7260.11358285055406842No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT7200.11264414930981992No Hit
CTGCTACTCTGGCTCCCAGAGACCACCGGAGACATTGTGTTGACGCAGTC7200.11264414930981992No Hit
CACTTAACCTGTCTGGGATGCCAGTGGCCCTGGTGGAGGCACCATAGATG7130.11154899785819666No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7010.10967159536969967No Hit
ACCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGC7000.10951514516232491No Hit
CAATAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTTAGTTCCATGTA6810.10654259122220466No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6710.10497808914845716No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6690.10466518873370767No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6680.10450873852633291No Hit
GTCTGGGATGCCAGTGGCCCTGGTGGAGGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTA6630.10372648748945916No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6620.10357003728208442No Hit
CTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT6600.10325713686733491No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG6460.10106683396408842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAC200.00783718450.92988612
CGTATGC1250.049.77662341
GACGTAG1250.048.97698226
GGTATCA4700.044.8139461
ACGTAGA1400.043.7431627
AGACGTA1400.043.7020525
CACGAGA1400.043.67468621
ATGCCGT1450.043.1169244
CGAGACG1500.043.03102523
TCGTATG1450.042.8426440
GTGGTAT1350.042.779381
CGTAGAG1450.042.2546528
ACGAGAC1500.040.76304222
TATACAC1600.040.3194923
ATCTCGT1450.040.26399237
AATCTCG1450.040.20672636
TGGTATC1450.039.807272
ACACATC1550.039.429596
CCCACGA1650.039.10993219
GGTACGT400.001443788138.87714868
Produced by FastQC (version 0.11.5)