FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639181
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26360.41240274663984067No Hit
GTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22080.34544205788344773No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA19460.30445210355126323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18890.2955344417309025No Hit
CTACCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTC16200.2534493359470948No Hit
GTCTGGGACAGACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATT16020.2506332322143493No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15720.2459397259931068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15700.2456268255783573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14560.22779150193763584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14280.22341089613114284No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14170.22168994385002058No Hit
CATCTATGGTGCCTCCACCAGGGCCACTGGCATCCCAGACAGGTTAAGTGGCAGTGGGTCTGGGACAGACTTCA13620.21308518244440933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13330.20854812643054157No Hit
GTCCCAGACCCACTGCCACTTAACCTGTCTGGGATGCCAGTGGCCCTGGT13080.20463687124617283No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12270.1919644044488181No Hit
CTACTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTACC12070.1888354003013231No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12010.1878966990570746No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTACTGCAGGGCCAGTCAGACTGTTAGCAG11780.18429834428745534No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTACTGCAGGGCCAGTCAGA11780.18429834428745534No Hit
TTGCTAGACAGTCTCGCACAATAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTTAGTTCCATGTACGCTGTG11230.1756935828818441No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTACTGC10840.16959202479422888No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10480.16395981732873788No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10180.15926631110749537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10130.15848406007062163No Hit
GTCTAGCAATGGCCGATATTACTTCGAGTTCTGGGGCCGTGGCACCCTAGTCACTGTCTCTTCAGCATCCCCGAC10120.15832760986324687No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10090.15785825924112262No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTCGCTGCTGCTAACAGTCTGA10050.15723245841162362No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10030.15691955799687413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9980.15613730696000036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9980.15613730696000036No Hit
GCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTTAACCTGT9910.15504215550837713No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9690.1516002509461326No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9450.14784544596913862No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTTAACCTGTCTGGGATGCCA9360.14643739410276588No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC9310.14565514306589214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9290.14534224265114265No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGA9100.1423696887110224No Hit
CTCTACTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTA8950.14002293560040113No Hit
GTTTATTACTGTCAGCAGTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8660.13548587958653338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8650.13532942937915865No Hit
GGTTAAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATAAGCAGA8630.13501652896440913No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8400.1314181741947899No Hit
GACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGGTAGTTCA8370.13094882357266566No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8320.1301665725357919No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8040.1257859667292989No Hit
ACCCTCTACTGCAGGGCCAGTCAGACTGTTAGCAGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCT7970.12469081527767564No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7880.1232827634113029No Hit
CACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGGTAGTTCACCTCTCACTTT7870.12312631320392814No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC7820.12234406216705439No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCACCGGAGACATTGTGTTGACGCAGTCTC7520.11765055594581192No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7390.11561670324994017No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGGCACCTTCAGCAG7300.11420865138356742No Hit
GGTTAAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTTT7250.11342640034669367No Hit
CTGCTACTCTGGCTCCCAGAGACCACCGGAGACATTGTGTTGACGCAGTC7240.11326995013931891No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7230.11311349993194417No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7190.11248769910244516No Hit
ATCCCAGACAGGTTAAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC6970.10904579454020066No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6950.10873289412545116No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6870.10748129246645316No Hit
ACCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGC6830.10685549163695417No Hit
CTCCCAGGCTCCTCATCTATGGTGCCTCCACCAGGGCCACTGGCATCCCA6750.10560388997795617No Hit
CACTTAACCTGTCTGGGATGCCAGTGGCCCTGGTGGAGGCACCATAGATG6710.10497808914845716No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6690.10466518873370767No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC6590.10310068665996017No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6530.10216198541571167No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6470.10122328417146316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)