FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388311
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74310.5352547087792289No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65300.4703557056019869No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA52880.38089448257631037No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44620.32139772716631937No Hit
GTATATTGCTGTCAGCAGTATGCCATCTCACATGGGTTCACTTTTGGCCA43700.3147709699051581No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42980.30958481204859717No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40730.2933780687468442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38860.2799084643138317No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT38640.2783238049687714No Hit
GTGCTAACACCAAGTCCTTGGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGT37980.2735698269335905No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36930.2660066800594391No Hit
ATATTGCTGTCAGCAGTATGCCATCTCACATGGGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA36170.2605324023219581No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36110.260100222500578No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35560.25613857413792734No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35000.2521048958050465No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT33590.24194867000261472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33220.23928356110410418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33120.2385632614018041No Hit
GCAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33010.23777093172927394No Hit
CTCCTGCAGGGCCAGTCAGAGTGCTAACACCAAGTCCTTGGCCTGGTACC32720.23568206259260352No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC30780.22170824836798092No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30420.2191151694397005No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29730.21414510149382954No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACAC29510.21256044214876926No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC27850.20060346709058707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27470.19786632822184655No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTGCTGTCAGCAGTATGCCATCTCA27170.19570542911494615No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA26960.19419279974011586No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26310.18951085167516502No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCC26160.1884304021217148No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGAACCCATGTGAGATGGCATACTG25900.18655762289573444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25550.184036573937684No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT25200.18151552497963352No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25070.18057913536664336No Hit
CACCAAGTCCTTGGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGC24030.17308801846272198No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA23800.17143132914743164No Hit
CAGCAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23550.16963057989168134No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23180.16696547099317086No Hit
GGCCAAAAGTGAACCCATGTGAGATGGCATACTGCTGACAGCAATATACTGCAAAATCTTCAGGCTCCAGTCTG23140.1666773511122508No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACACCAAGTCCTTG22740.16379615230305025No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA22580.16264367277937006No Hit
GTCCTTGGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCA22390.16127510334499978No Hit
GTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCACTGGCATCCCAGA22310.16069886358315968No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22120.15933029414878944No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21910.15781766477395914No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT21530.15508052590521865No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20760.14953421819750762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20690.14903000840589753No Hit
GTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG20350.14658098941807707No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTC20270.14600474965623697No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTC20180.14535647992416686No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19980.14391588051956658No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCACTGGCATCCCAGACAGGTT19680.14175498141266618No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18820.13556040397288505No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18700.13469604433012486No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18530.13347153483621466No Hit
GTGTTAGCACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTGTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA18290.13174281555069434No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17730.1277091372178136No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17590.1267007176345934No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16340.11769697135584174No Hit
GACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCC16170.11647246186193151No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16140.11625637195124146No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16110.11604028204055145No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15910.11459968263595116No Hit
CTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCACTGGCATCCCA15790.11373532299319102No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15780.11366329302296099No Hit
GGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACACCAAGTCCTTGGCCTGGTACCAGCAGAGAC15760.11351923308250096No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15740.11337517314204094No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15730.11330314317181092No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15680.11294299332066088No Hit
CCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACAC15580.11222269361836072No Hit
GCAGTATGCCATCTCACATGGGTTCACTTTTGGCCAGGGGACCAGGCTGG15390.11085412418399046No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15320.11034991439238039No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15300.11020585445192035No Hit
GGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA15170.10926946483893019No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15150.10912540489847015No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15080.10862119510686008No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15050.10840510519617003No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14940.10761277552363986No Hit
GTATGCCATCTCACATGGGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT14930.10754074555340987No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14720.10602811617857959No Hit
GGTCTCTGCTGGTACCAGGCCAAGGACTTGGTGTTAGCACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTGTCC14670.10566796632742952No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14640.10545187641673946No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTCCAGGCACC14360.1034350372502991No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14350.10336300728006909No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14330.10321894733960907No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14200.10228255772661889No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14170.10206646781592886No Hit
CCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCAC14100.10156225802431876No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14090.10149022805408875No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13890.10004962864948846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA200.00796183850.73012520
CGCTAAT3650.049.45960232
TCGTATG3700.049.39879640
CGTATGC3850.047.57026741
ACACTCG4050.043.51423327
TCGCTAA4150.043.44387431
GCTAATC3750.042.7435233
ACGAGAC4200.042.68541322
ACTCGCT4150.042.53171529
GGTATCA16000.042.077371
ATCTCGT3900.041.3398137
CACGAGA4350.041.2090521
TCTCCGA4400.040.72902311
TACACAT4450.040.2713935
CACTCGC4450.039.63574628
GTATCAA45050.039.1253661
AATCTCG4150.038.79417836
GCCGTCT4650.038.31447646
CTTATAC4800.038.0599331
TATACAC4800.038.039373
Produced by FastQC (version 0.11.5)