FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388311
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72540.5225054040485165No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56590.4076176015316453No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA49910.3595015814179964No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43190.3110974414234275No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42490.30605534350732655No Hit
GTATATTGCTGTCAGCAGTATGCCATCTCACATGGGTTCACTTTTGGCCA41710.3004370058293855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39500.28451838240855254No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39180.2822134233611921No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT38660.2784678649092314No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37290.2685997589877196No Hit
GTGCTAACACCAAGTCCTTGGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGT35740.25743511360206756No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35170.25332940529895676No Hit
ATATTGCTGTCAGCAGTATGCCATCTCACATGGGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA34980.2519608358645865No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33660.24245287979422478No Hit
GCAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32410.23344913351547314No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31790.22898327536121232No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT31690.22826297565891215No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31170.22451741720695148No Hit
CTCCTGCAGGGCCAGTCAGAGTGCTAACACCAAGTCCTTGGCCTGGTACC30530.21990749911223062No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29500.21248841217853925No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGC29400.2117681124762391No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28880.20802255402427844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28840.20773443414335838No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28430.20478120536392783No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACAC28240.20341263592955758No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28000.20168391664403726No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA27030.19469700953172597No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC26920.19390467985919582No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26740.19260814039505558No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTGCTGTCAGCAGTATGCCATCTCA26380.1900150614667751No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26160.1884304021217148No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGAACCCATGTGAGATGGCATACTG25250.1818756748307836No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25050.1804350754261833No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT24620.17733778670629274No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCC24430.1759692172719225No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA23590.16991869977260138No Hit
CAGCAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23020.16581299146949063No Hit
CACCAAGTCCTTGGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGC22580.16264367277937006No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA22320.1607708935533897No Hit
GTCCTTGGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCA21710.1563770653693589No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21580.15544067575636872No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21510.15493646596475863No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTC21480.15472037605406858No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACACCAAGTCCTTG21480.15472037605406858No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21320.15356789653038835No Hit
GGCCAAAAGTGAACCCATGTGAGATGGCATACTGCTGACAGCAATATACTGCAAAATCTTCAGGCTCCAGTCTG21170.15248744697693817No Hit
GTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCACTGGCATCCCAGA20880.15039857784026778No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19980.14391588051956658No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19680.14175498141266618No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTC19570.14096265174013606No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19490.14038641197829593No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19450.14009829209737587No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCACTGGCATCCCAGACAGGTT19130.13779333305001545No Hit
GTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG18500.13325544492552463No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18250.13145469566977427No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18190.1310225158483942No Hit
GTGTTAGCACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTGTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA17700.12749304730712355No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17680.12734898736666353No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16940.12201876956964254No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16220.11683261171308158No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16210.11676058174285156No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15930.11474374257641119No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15480.1115023939160606No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15460.11135833397560056No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15460.11135833397560056No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15390.11085412418399046No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15210.10955758471985025No Hit
GACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCC15170.10926946483893019No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15000.10804495534501996No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14990.10797292537478995No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14930.10754074555340987No Hit
GTATGCCATCTCACATGGGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT14640.10545187641673946No Hit
CTCCCAGGCTCCTCATCTATGATACGTCCAGCAGGGCCACTGGCATCCCA14630.10537984644650947No Hit
GCAGTATGCCATCTCACATGGGTTCACTTTTGGCCAGGGGACCAGGCTGG14580.1050196965953594No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACGCAGTCTCCAGGCACC14500.10444345683351929No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14280.102858797488459No Hit
GGACAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGCTAACACCAAGTCCTTGGCCTGGTACCAGCAGAGAC14250.10264270757776896No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14210.1023545876968489No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14190.10221052775638886No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14090.10149022805408875No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13990.10076992835178862No Hit
GGTCTCTGCTGGTACCAGGCCAAGGACTTGGTGTTAGCACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTGTCC13930.10033774853040853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.039.158311
GTATCAA45950.036.983831
AACGCAG52750.031.792976
TCAACGC52600.031.749614
ATCAACG52850.031.663263
CAACGCA53350.031.4320145
TATCAAC54050.031.0918432
ACGCAGA55700.030.1697247
CGCAGAG56750.029.6709778
AGAGTAC57150.029.40426411
GCAGAGT58400.029.0060129
AATGGTA6400.027.6982970
TGTTAGA17600.026.8589570
GATTCAC3900.025.988791
ATGGCAA5650.025.5648670
ATTCACT3750.025.1964632
CAGAGTA66950.025.1505310
CGGGATA1101.3889003E-624.56058917
AGTACGG51950.024.3624613
GAGTACG52050.024.31127212
Produced by FastQC (version 0.11.5)