FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098662
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC43680.397574504260637No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACAC38600.35133644378343837No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG35720.325122740205814No Hit
CCTATGAGCTGACTCAGCCGCTCTCAGTGTCAGTGACCCTGGGACAGACG33160.30182167035903673No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT33070.30100249212223595No Hit
GTAATACACGGCCGAGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTGCTGA32040.2916274523010717No Hit
CAGTAATACACGGCCGAGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTG30150.27442470932825563No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28700.2612268377353545No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28650.2607717387149096No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCATT28640.2606807189108206No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGG27990.2547644316450373No Hit
GTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC26140.2379257678885772No Hit
GGATTACCTGTGGGGGAAACAACATTGGCATTTACAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC25480.23191846081870496No Hit
GCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCT23970.21817447040126992No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22980.20916350979646153No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTC22850.20798025234330486No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT22710.20670597508605926No Hit
GTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGG22530.2050676186124577No Hit
CCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGGGATCCCT22400.20388436115930103No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC21470.1954195193790265No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20810.18941221230915423No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20380.18549836073332837No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTGTAAATG20210.1839510240638158No Hit
GTGTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATAT20020.18222164778612532No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19700.17930901405527813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19400.17657841993260892No Hit
GTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATATATGGGGCCAAGGGACAATGGTCACCGT19260.1753041426753633No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18140.16510992461739826No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCG17410.1584654789189032No Hit
GGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG17110.15573488479623399No Hit
GCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCATCCC17060.1552797857757891No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16870.1535504094980986No Hit
GTCCCACACCTGACAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGGCCGTGTTCCC16820.15309531047765373No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16730.15227613224085296No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16560.15072879557134042No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16140.14690596379960352No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG16110.14663290438733662No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16100.1465418845832476No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15910.14481250830555714No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15710.14299211222377767No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15570.141717834966532No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15460.1407166171215533No Hit
CCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCA15080.1372578645661723No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14980.13634766652528257No Hit
GGGTATATCTATTTCAGGGGGAGCACCACCTCCAAGTCCTCCCTCAAGAGTCGAGCCACCATGTCAGCAGACACG14830.13498236946394795No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14820.134891349659859No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14350.13061341886767724No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14350.13061341886767724No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGG13860.12615344846731752No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT13590.12369591375691524No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13490.1227857157160255No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13440.12233061669558064No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13390.12187551767513577No Hit
GACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTG13380.12178449787104678No Hit
CAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGC13230.12041920080971216No Hit
GTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGG13110.11932696316064449No Hit
GTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAA12950.11787064629522091No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA12740.11595923040935248No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGC12680.11541311158481862No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12670.11532209178072965No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG12410.11295557687441633No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG12140.11049804216401406No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12110.11022498275174712No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG11980.10904172529859046No Hit
GTCCCAGGGTCACTGACACTGAGAGCGGCTGAGTCAGCTCATAGGAGGCC11970.10895070549450149No Hit
ACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGC11810.10749438862907791No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11800.10740336882498894No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11780.10722132921681099No Hit
GTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGGGATCCCTGAGCG11750.10694826980454407No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11740.10685725000045511No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC11670.10622011137183228No Hit
CTCTCAGTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCATTTACAATGTG11670.10622011137183228No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT11660.1061290915677433No Hit
CAGTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCATTTACAATGTGCA11440.1041266558777859No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11370.10348951724916307No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11130.10130504195102771No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11120.10121402214693873No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11110.10112300234284975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)