FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098662
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC42690.3885635436558286No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACAC35540.32348438373221244No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG33310.3031869674203713No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT32830.2988180168241006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31660.28816869974569065No Hit
CCTATGAGCTGACTCAGCCGCTCTCAGTGTCAGTGACCCTGGGACAGACG30920.2814332342431066No Hit
GTAATACACGGCCGAGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTGCTGA30650.27897569953270435No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28830.2624100951885111No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGG27530.25057752065694455No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26950.24529837201978408No Hit
GGATTACCTGTGGGGGAAACAACATTGGCATTTACAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26860.24447919378298327No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCATT26370.2400192233826236No Hit
CAGTAATACACGGCCGAGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTG26350.23983718377444566No Hit
GCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCT24000.21844752981353682No Hit
CCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGGGATCCCT23640.21517081686633377No Hit
GTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC22760.2071610741065041No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22270.2027011037061444No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT22210.20215498488161057No Hit
GTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGG20340.18513428151697248No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20060.18258572700248119No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTGTAAATG20020.18222164778612532No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTC19860.1807653309207017No Hit
GTGTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATAT19680.1791269744471002No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19670.17903595464301122No Hit
GTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATATATGGGGCCAAGGGACAATGGTCACCGT19440.17694249914896484No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19260.1753041426753633No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19240.17512210306718537No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC19220.17494006345900742No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCG17860.162561370102907No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17260.15710018185756858No Hit
GGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG17070.15537080557987806No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16820.15309531047765373No Hit
GTCCCACACCTGACAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGGCCGTGTTCCC16630.15136593419996322No Hit
GCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCATCCC16380.1490904390977389No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16170.14717902321187043No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16120.14672392419142558No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16100.1465418845832476No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15450.14062559731746435No Hit
CCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCA15240.1387141814315959No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG15100.13743990417435026No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14970.13625664672119359No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14920.13580154770074873No Hit
GGGTATATCTATTTCAGGGGGAGCACCACCTCCAAGTCCTCCCTCAAGAGTCGAGCCACCATGTCAGCAGACACG14730.13407217142305822No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14540.13234279514536773No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14150.12879302278589777No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT13510.12296775532420344No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGG13210.12023716120153423No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13090.11914492355246656No Hit
GTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGG13080.11905390374837757No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12800.11650534923388631No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12780.11632330962570836No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA12720.11577719080117452No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12270.1116812996171707No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGC12230.11131722040081479No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT12220.11122620059672583No Hit
GTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAA12150.11058906196810302No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12130.11040702235992506No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12120.1103160025558361No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12100.11013396294765815No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG12090.11004294314356919No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC11930.10858662627814558No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG11840.10776744804134485No Hit
ACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGC11630.1058560321554764No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11580.10540093313503153No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11500.10467277470231973No Hit
CTCTCAGTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCATTTACAATGTG11450.10421767568187486No Hit
CAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGC11390.10367155685734103No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11360.1033984974450741No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11330.10312543803280719No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11240.10230625979600642No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11120.10121402214693873No Hit
GTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGGGATCCCTGAGCG11100.10103198253876078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)