FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473817
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA14480.3056032181200759No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14090.29737219221767053No Hit
GGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCTAAACTCCTGATCTATAAAGCATC13950.29441746497065324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13940.29420641302443773No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9060.19121306327126297No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8990.1897356996477543No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8550.18044941401427134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8420.17770573871346954No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8070.1703189205959263No Hit
GTGTATTACTGTGCGAGAGATCGGGAGGTGGTGACTAAATACTACTTTGA7920.16715314140269344No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7830.16525367388675374No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7410.15638949214570183No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7060.14900267402815856No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6970.14710320651221886No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6930.14625899872735676No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6850.14457058315763258No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6800.14351532342655499No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6470.13655060920144277No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6230.13148536249227022No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6160.13000799886876158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6110.12895273913768396No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC6060.12789747940660637No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTCACAGTTTC6000.1266311677293132No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5830.12304328464364934No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5750.12135486907392516No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5600.11818908988069234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCTGAGGGAAA5470.11544541457989056No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5460.11523436263367502No Hit
GGTTACAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGC5420.11439015484881294No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5420.11439015484881294No Hit
GTGGGAGACAGAGTCATCATTACTTGTCGGGCGAGTCAGGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAG5330.11249068733287323No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5320.11227963538665772No Hit
CTAATACCCTGACTCGCCCGACAAGTAATGATGACTCTGTCTCCCACAGA5250.11080227176314907No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTACAGATGCGACAT5210.10995806397828696No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5130.1082696484085628No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5060.10679228478505415No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4840.10214914196831267No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT4820.10172703807588163No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4800.10130493418345059No Hit
GTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCT4790.10109388223723505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1700.050.1176071
CGTATGC1600.049.73557341
TTATACA1750.048.6651232
ATCTCCG1650.047.48536310
ATGCCGT1700.047.01656344
CTTGCGT306.176842E-446.19591542
AACTTAT556.464246E-445.76367670
ATCTCGT1450.045.0752937
CACGAGA1750.044.78609521
ACGAGAC1750.044.78609522
TATACAC2000.044.2852633
GTGGTAT1700.044.1034971
CCGTCTT1850.043.41003447
TCGTATG1850.042.95469740
ACCGAGG1750.042.8796127
CTTGAAA2000.042.51636557
TCTCCGA1850.042.35181411
CCCACGA1900.041.24599519
CGTCTTC1950.041.22400748
GTATGCC1950.040.8656242
Produced by FastQC (version 0.11.5)