FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473817
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15720.3317736594508006No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14310.302015335034412No Hit
GGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCTAAACTCCTGATCTATAAAGCATC13120.27690015343476493No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12040.2541065432434885No Hit
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATACA11550.24376499787892794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10000.21105194621552203No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8200.17306259589672807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8000.16884155697241762No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7620.1608215830162278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7280.15364581684490003No Hit
GTGTATTACTGTGCGAGAGATCGGGAGGTGGTGACTAAATACTACTTTGA7070.14921372597437407No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7050.14879162208194305No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6790.14330427148033945No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6770.14288216758790842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6760.14267111564169288No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6280.13254062222334784No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6160.13000799886876158No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6140.12958589497633052No Hit
GTGGGAGACAGAGTCATCATTACTTGTCGGGCGAGTCAGGGTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAG6110.12895273913768396No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6000.1266311677293132No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC5880.12409854437472696No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5670.11966645350420099No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5600.11818908988069234No Hit
GGTTACAGATGCGACATCCAGATGACCCAGTCTCCATCTTCCGTGTCTGC5570.11755593404204577No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5540.11692277820339919No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTCACAGTTTC5400.1139680509563819No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCTGAGGGAAA5370.11333489511773531No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5310.11206858344044221No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5220.1101691159245025No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5180.10932490813964041No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC5100.10763649256991623No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5060.10679228478505415No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTACAGATGCGACAT4990.10531492116154549No Hit
CTAATACCCTGACTCGCCCGACAAGTAATGATGACTCTGTCTCCCACAGA4980.10510386921532998No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4910.10362650559182132No Hit
GTATTAGTAGCTGGCTAGCCTGGTATCAGCAGAAGCCAGGGAGAGCCCCT4850.10236019391452818No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATAGCATAAATTGGGTCCGCCAGGCTCCAGGGAAGGGG4800.10130493418345059No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4760.10046072639858848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCG100.009844965129.9044370
GGTACGG200.0077954550.9966774
TCTGGAG4450.039.40921470
GTATCAA12400.037.0489041
CGGTACC709.010073E-734.83596445
TTAACGG701.0100212E-634.33109335
CACATGT807.795461E-834.1238228
GGGTACT601.4814415E-534.0013737
TTGCGGC400.002786788634.0013739
ATCAACG13750.032.885133
TAACCGT751.4154884E-632.87976553
TATCAAC14000.032.5476042
CAACGCA14300.032.0991975
GTGGTAT1600.031.9032231
TCAACGC14300.031.6203164
AACGCAG14550.031.5476656
CGTGCAA802.2405402E-631.00105957
GCGGTAC901.8530955E-730.91849544
AGGTAGC952.1316919E-730.427964
TAACGGC802.8384875E-630.07496336
Produced by FastQC (version 0.11.5)