FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200780
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCGTCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA64730.5390662735888339No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG56820.4731924249237995No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39930.3325338529955529No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC39320.32745382168257303No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35570.2962241209880245No Hit
GTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32540.2709905228268292No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32500.2706574060194207No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC32160.26782591315644827No Hit
GTATTAATTCCGATGTTGGAGCCGCTTCCAGAACAAGAGATGGCGACCCT30560.2545012408601076No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT28680.23884475091190727No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC27840.2318492979563284No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC27140.22601975382667933No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26560.2211895601192558No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26160.21785839204517066No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG25940.2160262496044238No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25650.21361115275071205No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24830.20678225819883742No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24190.20145238928030115No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23930.19928713003214577No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23530.19595596195806061No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23090.19229167707656689No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCGC22870.19045953463582005No Hit
GAATTAATACTGTAAACTGGTACCAACATCTCTCAGGAACGGCCCCCAAA22830.19012641782841153No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22720.18921034660803812No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22590.1881277169839604No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22330.18596245773580505No Hit
TTATTACTGTGCGTCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCG22260.18537950332284014No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21910.18246473125801563No Hit
ATACCACACCATTCAGGCTGTCATCCCATGACGCACAGTAATAATCAGCC21830.1817984976431986No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21560.17954995919319108No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT21540.17938340078948684No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20500.17072236379686537No Hit
GAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20330.16930661736537916No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCGTCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC20300.16905677975982278No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20160.16789087093389296No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG20000.1665584037042589No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19580.16306067722646947No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19540.16272756041906095No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19510.16247772281350456No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA19380.16139509318942685No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT19230.16014590516164492No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18910.1574809707023768No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTA18740.1560652242708906No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18620.15506587384866505No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC18140.15106847215976282No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17600.14657139525974783No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17570.14632155765419144No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT16740.1394093839004647No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16410.13666117023934443No Hit
GGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16390.13649461183564016No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG16140.13441263178933693No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC15740.13108146371525176No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15710.13083162610969537No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15540.12941587967820917No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC15330.12766701643931444No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC15270.12716734122820167No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCGT15200.12658438681523676No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14930.12433584836522926No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14670.1221705891170739No Hit
GTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTACAGTATTAATT14610.12167091390596113No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG14520.12092140108929195No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14430.12017188827262279No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14380.11975549226336214No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14290.11900597944669297No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC13920.1159246489781642No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13830.11517513616149504No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA13760.11459218174853013No Hit
CCATTCAGGCTGTCATCCCATGACGCACAGTAATAATCAGCCTCATCCTC13570.11300987691333966No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13530.11267676010593114No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13500.11242692250037477No Hit
CTCTTGTTCTGGAAGCGGCTCCAACATCGGAATTAATACTGTAAACTGGTACCAACATCTCTCAGGAACGGCCCC13370.11134429287629707No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCGCCATCTCTTGTTCTGG12980.10809640400406403No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG12940.1077632871966555No Hit
CATTTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC12830.10684721597628208No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC12770.10634754076516931No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC12720.10593114475590866No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG12690.10568130715035227No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC12610.10501507353553523No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12550.10451539832442246No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12500.10409900231516181No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12460.1037658855077533No Hit
GTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTACAGTATTAATTCCGATGTTGGAG12450.10368260630590116No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA12440.10359932710404904No Hit
GATTATTAGTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGG12390.10318293109478839No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12230.10185046386515433No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12170.10135078865404154No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12110.10085111344292877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4450.056.82876270
ATTCGTT200.00730019851.85524442
TATCGTA306.478604E-445.75674438
GGTATCA10050.044.7965661
CGAATAT405.335854E-542.6555334
TGTCCGA350.001016522541.756263
TATACAC4350.040.445883
GTATCAA27350.039.1101041
GGCGAAT4250.038.44364532
CTTATAC4250.038.230631
CGTATGC4000.037.9432341
TCGTATG3750.037.63019640
TTATACA4500.037.5939252
TGGTATC2750.036.91042
CGAGACT4500.036.1127323
GTATGCC4500.036.10661342
ACGAGAC4550.035.70993422
TCTCGTA3500.034.3175638
ATATCGT400.002685791334.26256637
AGGCGAA4800.034.00018731
Produced by FastQC (version 0.11.5)