FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200780
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCGTCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA61170.5094188777294758No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45840.38175186129016137No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC42310.35235430303635973No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38280.31879278468995154No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG36300.3023035027232299No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35590.2963906793917287No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31330.26091373940272156No Hit
GTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31250.2602475057879045No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30040.25017072236379684No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC30010.24992088475824045No Hit
GTATTAATTCCGATGTTGGAGCCGCTTCCAGAACAAGAGATGGCGACCCT28580.23801195889338594No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC27550.22943420110261664No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26820.2233548193674112No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCGC26010.2166092040173887No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25520.21252852312663434No Hit
GAATTAATACTGTAAACTGGTACCAACATCTCTCAGGAACGGCCCCCAAA25480.21219540631922584No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25260.210363263878479No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25220.21003014707107046No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24680.2055330701710555No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23540.19603924115991272No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG23060.1920418394710105No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC22870.19045953463582005No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22820.19004313862655942No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22530.18762804177284764No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21990.18313096487283267No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21570.17963323839504322No Hit
TTATTACTGTGCGTCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCG21500.1790502839820783No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21480.17888372557837404No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCGTCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC21130.17596895351354952No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20980.1747197654857676No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20500.17072236379686537No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20360.16955645497093555No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19680.16389346924499076No Hit
GAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19670.16381019004313863No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19660.16372691084128652No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19530.1626442812172088No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT19160.15956295074868004No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18750.15614850347274273No Hit
ATACCACACCATTCAGGCTGTCATCCCATGACGCACAGTAATAATCAGCC18370.1529838938023618No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18270.1521511017838405No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18220.15173470577457987No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18080.15056879694865005No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17520.14590516164493078No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTA17500.14573860324122653No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17340.14440613601159247No Hit
CTCTTGTTCTGGAAGCGGCTCCAACATCGGAATTAATACTGTAAACTGGTACCAACATCTCTCAGGAACGGCCCC16790.13982577990972533No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16700.13907626709305618No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16560.13791035826712636No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT16420.13674444944119657No Hit
GGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16100.1340795149819284No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15480.1289162044670964No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15150.12616799080597613No Hit
GTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTACAGTATTAATT14680.12225386831892604No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14600.121587634704109No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG14530.12100468029114408No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCGT14510.12083812188743984No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC14210.11833974583187595No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14040.11692399940038975No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13970.11634104498742484No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG13940.11609120738186846No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13930.11600792818001632No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13830.11517513616149504No Hit
CATTTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC13710.11417578573926948No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13640.11359283132630457No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCGCCATCTCTTGTTCTGG13560.11292659771148753No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC13450.1120105264911141No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA13280.1105947800596279No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC13230.11017838405036726No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13180.10976198804110661No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13120.10926231282999384No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA12950.10784656639850763No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC12940.1077632871966555No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG12820.10676393677442997No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12740.10609770315961292No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12710.10584786555405652No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT12710.10584786555405652No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC12590.10484851513183097No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC12580.10476523592997886No Hit
GTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTACAGTATTAATTCCGATGTTGGAG12080.10060127583737238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)