FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938832
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGCTGGCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT47370.5045631167237589No Hit
CTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGGTGATGAGGAGAGTGAACTCTGTC33040.35192664928336487No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32490.3460683061506212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29680.3161374985087854No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24950.26575574756718984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20520.21856945651618181No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19820.2111133834381444No Hit
GTCTGGGACAGAGTTCACTCTCCTCATCACCAGCCTGCAGTCTGAGGATT17520.18661485761030727No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17030.18139560645568112No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16820.17915878453226988No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16820.17915878453226988No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16720.1780936312354074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16360.17425907936670246No Hit
GTAATACACGGCCGTGTCGTCAGATCTTAGGTTCCTCAGCTCCATGTAGA15860.16893331288239005No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAG15480.1648857303543126No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTA15400.1640336077168226No Hit
GAGTTCACTCTCCTCATCACCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAGCTGG15260.16254239310121513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15260.16254239310121513No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCACCAACCTGGCCAGGCTCCCAGACT15130.1611576938152939No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15060.16041208650749017No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14870.15838829524345144No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14460.15402116672631527No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14040.1495475228794928No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13990.14901494623106157No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13900.14805630826388533No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA13300.14166538848271043No Hit
GTCTACATGGAGCTGAGGAACCTAAGATCTGACGACACGGCCGTGTATTA13290.1415588731530242No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13270.14134584249365167No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13140.13996114320773045No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13060.13910902057024047No Hit
GGCCAAAAGTGGGCCAGCTATTATACTGCTGACAGTAATAAACCGCAAAA12890.13729825996557424No Hit
CCTCATCACCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAGCTGGCCCACTTTTGG12610.1343158307343593No Hit
GTGTATTACTGTGCGAGACATGACACCTCGGGCAAGGTGTGGTTCGGCCC12300.13101385551408556No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGACAAATCATCCCTATATTTGAT12210.13005521754690935No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12110.12899006425004686No Hit
GTTTATTACTGTCAGCAGTATAATAGCTGGCCCACTTTTGGCCAGGGGAC12060.12845748760161563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12040.12824445694224312No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCCTCTGGCGGCACCTTCAGCAC12020.12803142628287065No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG11840.12611415034851817No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11760.12526202771102815No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11500.12249262913918571No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11450.12196005249075446No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11390.12132096051263698No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11320.12057535320483324No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA11270.120042776556402No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA11100.11823201595173578No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11080.1180189852923633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11000.11716686265487329No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10980.11695383199550079No Hit
GCTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGGTGATGAGGAGAGTGAACT10940.1165277706767558No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10840.11546261737989331No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10720.11418443342365832No Hit
GTACCAGCACCAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGC10620.11311928012679585No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10490.11173458084087462No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10490.11173458084087462No Hit
GTCTGGGAGCCTGGCCAGGTTGGTGCTGGTACCAGGCTAAGTTGCTGCCA10440.11120200419244337No Hit
GTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCACCAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTG10370.11045639688463964No Hit
GTAATAAACCGCAAAATCCTCAGACTGCAGGCTGGTGATGAGGAGAGTGAACTCTGTCCCAGACCCACTGCCA10070.10726093699405218No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10010.10662184501593469No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9910.1055566917190722No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT9870.1051306304003272No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTTAGGTTCCTCAGCTCCATGTA9840.10481108441126848No Hit
GCACCAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT9790.10427850776283723No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTGGTGCTGGTACCAGGC9660.10289380847691601No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9610.10236123182848476No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9510.10129607853162227No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9480.10097653254256353No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTGGTGCTGGTACCAGG9440.10055047122381852No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9440.10055047122381852No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9440.10055047122381852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)