FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938832
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGCTGGCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT45300.4825144434787054No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34800.3706733473081446No Hit
CTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGGTGATGAGGAGAGTGAACTCTGTC31250.33286040526952637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27440.2922780646590657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25110.26745999284216987No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22410.23870085382688275No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19340.2060006476132045No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17700.18853213354465975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17560.18704091892905228No Hit
GTCTGGGACAGAGTTCACTCTCCTCATCACCAGCCTGCAGTCTGAGGATT17050.1816086371150536No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16860.17958484585101486No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCACCAACCTGGCCAGGCTCCCAGACT16030.17074407348705625No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15520.1653117916730576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15030.16009254051843141No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAG14920.15892087189188267No Hit
GAGTTCACTCTCCTCATCACCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAGCTGG14630.15583192733098147No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAGCAACTTAGCCTGGTA14370.15306252875913903No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA14180.1510387374951003No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14110.15029313018729656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14010.14922797689043407No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13760.14656509364827786No Hit
GTAATACACGGCCGTGTCGTCAGATCTTAGGTTCCTCAGCTCCATGTAGA13520.1440087257358079No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13410.14283705710925915No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13370.14241099579051417No Hit
GGCCAAAAGTGGGCCAGCTATTATACTGCTGACAGTAATAAACCGCAAAA13260.14123932716396545No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13150.1400676585374167No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13080.13932205122961297No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13010.13857644392180923No Hit
GTCTACATGGAGCTGAGGAACCTAAGATCTGACGACACGGCCGTGTATTA12550.13367673875624178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12500.13314416210781058No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA12420.13229203947032056No Hit
GTGTATTACTGTGCGAGACATGACACCTCGGGCAAGGTGTGGTTCGGCCC11990.1277118802938119No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11940.12717930364538063No Hit
GTTTATTACTGTCAGCAGTATAATAGCTGGCCCACTTTTGGCCAGGGGAC11840.12611415034851817No Hit
CCTCATCACCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAGCTGGCCCACTTTTGG11830.12600763501883191No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11830.12600763501883191No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11520.12270565979855821No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11410.12153399117200948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11380.12121444518295073No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGACAAATCATCCCTATATTTGAT11300.12036232254546075No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11020.11737989331424578No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10810.11514307139083457No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCCTCTGGCGGCACCTTCAGCAC10610.11301276479710959No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG10570.11258670347836461No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10520.11205412682993336No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10420.11098897353307088No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10310.10981730490652215No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10220.10885866693934591No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10150.10811305963154218No Hit
GTCTGGGAGCCTGGCCAGGTTGGTGCTGGTACCAGGCTAAGTTGCTGCCA10040.10694139100499345No Hit
GCTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGGTGATGAGGAGAGTGAACT10020.10672836034562096No Hit
GTCAGAGTCTTGGCAGCAACTTAGCCTGGTACCAGCACCAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTG9890.10534366105969971No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9840.10481108441126848No Hit
GTAATAAACCGCAAAATCCTCAGACTGCAGGCTGGTGATGAGGAGAGTGAACTCTGTCCCAGACCCACTGCCA9700.10331986979566099No Hit
GTACCAGCACCAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGC9640.10268077781754349No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9570.10193517050973977No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9460.10076350188319103No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG9450.10065698655350479No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9420.10033744056444603No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9410.1002309252347598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)