FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030508
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42140.4089245304257706No Hit
GCCTTGGGCTGACCTAGGACGGTTAGTTTGGTCCCTCCGCCGAAAACCAC36120.3505067403649462No Hit
GTCTTGGGCTCAGGCCTGATTCGGAACCTGACCATTAAGAATGTCCAGGA33390.324014951848991No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30460.2955823729655665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27790.269672821559852No Hit
CTGTGGGACAGACCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCG27650.26831426830262356No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTTAGTTTGGTCCCTC25870.25104123403214723No Hit
GTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGACATTCTTAATGGT25790.25026491788515953No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24870.241337282194801No Hit
CACCTGCACCCTGAGGAGCGACTACAGTAATTATAGAGTGGACTGGCACC24380.23658234579450135No Hit
AGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCG23180.22493760358968587No Hit
CCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCGGAGGGACCAAACTAACCGTCCTAGGTCAGCCCAAGG21520.2088290435396911No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20740.20125996110656103No Hit
GACCTAGGACGGTTAGTTTGGTCCCTCCGCCGAAAACCACATTGAAATTC20590.1998043683309591No Hit
GTGGTAGTCACCCTCATCCTCTTCCTGGACATTCTTAATGGTCAGGTTCC20570.19961028929421218No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCACTGGTGGGATGTTGGGATCCAAGGGGGATGGCATCC20550.19941621025746525No Hit
GACTACCACTGTGGGACAGACCTTAGCAGTGGGGAGAATTTCAATGTGGT20440.19834877555535715No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20330.19728134085324908No Hit
CTGCTAAGGTCTGTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGAC19330.18757738901590285No Hit
GCTAAGGTCTGTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGACAT19300.18728627046078244No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18940.1837928477993378No Hit
GACCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCGGAGGGACCAA18920.18359876876259087No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18770.18214317598698893No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18700.1814638993583747No Hit
GCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCTTGGATCCCAACA18620.180687583211387No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18010.17476817259060579No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17930.1739918564436181No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17770.1724392241496427No Hit
GCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAG17550.17030435474542652No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17010.16506422075325955No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16620.1612796795366945No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16230.15749513832012949No Hit
GGATGAGGGTGACTACCACTGTGGGACAGACCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCGGAGGGAC16130.15652474313639486No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15900.15429283421380524No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15810.15341947854844407No Hit
GTAATTATAGAGTGGACTGGCACCAGCAGAGACCAGGGAAGGGCCCCCGA15370.14914973974001172No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15290.14837342359302402No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15020.14575335659694055No Hit
CCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAGCGACTACAGT15020.14575335659694055No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14710.1427451315273632No Hit
ACCTAGGACGGTTAGTTTGGTCCCTCCGCCGAAAACCACATTGAAATTCT14640.14206585489874896No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14550.1411924992333878No Hit
TCCCTGGTCTCTGCTGGTGCCAGTCCACTCTATAATTACTGTAGTCGCTC14430.14002802501290626No Hit
ATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAGCGA14370.13944578790266549No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13970.135564207167727No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13830.13420565391049852No Hit
CCTCTGCTCCTCACCCTCCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCC13730.13323525872676387No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13640.13236190306140272No Hit
CTCCCAGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACC13260.12867440136321115No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12770.1239194649629115No Hit
CTGGTGGGATGTTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCA12710.12333722785267073No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12550.12178459555869532No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTTAGT12490.12120235844845453No Hit
CCCTCATCCTCTTCCTGGACATTCTTAATGGTCAGGTTCCGAATCAGGCC12430.12062012133821377No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12400.12032900278309339No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT12320.11955268663610569No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG12090.11732077771351605No Hit
GTCCCTCTCCCAGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTG12080.11722373819514258No Hit
ACCATGGCCTGGGCTCCTCTGCTCCTCACCCTCCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCCTATACTGACT12030.11673854060327528No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11340.11004281383550638No Hit
GTCCAGGAAGAGGATGAGGGTGACTACCACTGTGGGACAGACCTTAGCAG11280.1094605767252656No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11070.10742274683942289No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10930.10606419358219442No Hit
CCTGCACCCTGAGGAGCGACTACAGTAATTATAGAGTGGACTGGCACCAG10920.10596715406382094No Hit
CTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAGCGACTACAGTAATTATAGAGTGGA10890.10567603550870057No Hit
CTGAAGAACCCAGCATTGCAGCAGCTCCACCATGGCCTGGGCTCCTCTGC10870.10548195647195363No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTTAGTTTGGTC10760.10441452176984554No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10510.10198853381050899No Hit
GGTCTGTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGACATTCTTA10430.1012122176635213No Hit
TTCTTAATGGTCAGGTTCCGAATCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCTTGGAT10420.10111517814514782No Hit
CTATAATTACTGTAGTCGCTCCTCAGGGTGCAGGTGAGTGTGACCGAGGC10380.10072702007165397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.042.2861441
CGTATGC1850.039.18101541
TACGTAT650.001485402238.65303470
TCGTATG1800.038.32925440
GTATCAA25400.036.6200031
TGGTATC3200.036.326862
GTGGTAT3300.036.2691461
TATCAAC27850.032.778192
ATCAACG28400.032.1434023
TCTCGTA1850.031.6358238
TCAACGC28950.031.5327324
CAACGCA29400.031.0515925
AACGCAG29850.030.5834796
ACTAGGC2250.030.41914427
CACGAGA2250.030.38962421
ATGCCGT2350.029.49142344
ACGCAGA31100.029.2400447
AGAGTAC31700.028.57875811
CGCAGAG31700.028.5787588
ATCTCGT2050.028.52152637
Produced by FastQC (version 0.11.5)