FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030508
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40940.3972797882209551No Hit
GCCTTGGGCTGACCTAGGACGGTTAGTTTGGTCCCTCCGCCGAAAACCAC34430.33410706175983107No Hit
GTCTTGGGCTCAGGCCTGATTCGGAACCTGACCATTAAGAATGTCCAGGA33120.3213948848529075No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29410.28539322353635294No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27000.2620066996083485No Hit
CTGTGGGACAGACCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCG26580.2579310398366631No Hit
AGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCG25810.2504589969219065No Hit
CACCTGCACCCTGAGGAGCGACTACAGTAATTATAGAGTGGACTGGCACC25190.24444254678275182No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24830.24094912412130717No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTTAGTTTGGTCCCTC24200.23483563446377903No Hit
GTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGACATTCTTAATGGT23090.22406424792432467No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22310.21649516549119463No Hit
CCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCGGAGGGACCAAACTAACCGTCCTAGGTCAGCCCAAGG20870.20252147484541605No Hit
GTGGTAGTCACCCTCATCCTCTTCCTGGACATTCTTAATGGTCAGGTTCC20380.1977665384451164No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19910.19320568108156366No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCACTGGTGGGATGTTGGGATCCAAGGGGGATGGCATCC19490.18913002130987824No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19390.1881596261261436No Hit
GACTACCACTGTGGGACAGACCTTAGCAGTGGGGAGAATTTCAATGTGGT19230.18660699383216822No Hit
GACCTAGGACGGTTAGTTTGGTCCCTCCGCCGAAAACCACATTGAAATTC19150.18583067768518052No Hit
GACCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCGGAGGGACCAA18770.18214317598698893No Hit
CTGCTAAGGTCTGTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGAC18690.18136685984000123No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18220.1768060024764485No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18100.17564152825596696No Hit
GCTAAGGTCTGTCCCACAGTGGTAGTCACCCTCATCCTCTTCCTGGACAT17970.17438001451711194No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17480.1696250781168123No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17450.1693339595616919No Hit
GCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAG17100.16593757641862072No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17060.16554941834512688No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16810.1631234303857903No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16720.16225007472042915No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16450.15963000772434566No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15890.15419579469543176No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15630.15167276721772174No Hit
GGATGAGGGTGACTACCACTGTGGGACAGACCTTAGCAGTGGGGAGAATTTCAATGTGGTTTTCGGCGGAGGGAC15590.1512846091442279No Hit
GCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCTTGGATCCCAACA15570.15109053010748097No Hit
ATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAGCGA15390.14934381877675865No Hit
GTAATTATAGAGTGGACTGGCACCAGCAGAGACCAGGGAAGGGCCCCCGA15280.14827638407465057No Hit
CCTCTGCTCCTCACCCTCCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCC14890.14449184285808553No Hit
CTCCCAGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACC14740.1430362500824836No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14720.14284217104573665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14430.14002802501290626No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14270.13847539271893083No Hit
CCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAGCGACTACAGT14190.13769907657194316No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13510.13110038932254772No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13450.13051815221230695No Hit
ACCTAGGACGGTTAGTTTGGTCCCTCCGCCGAAAACCACATTGAAATTCT13300.129062559436705No Hit
CTGGTGGGATGTTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCA13260.12867440136321115No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12980.1259572948487542No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12760.12382242544453803No Hit
TCCCTGGTCTCTGCTGGTGCCAGTCCACTCTATAATTACTGTAGTCGCTC12700.12324018833429724No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12610.1223668326689361No Hit
ACCATGGCCTGGGCTCCTCTGCTCCTCACCCTCCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCCTATACTGACT12290.11926156808098531No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12230.11867933097074453No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12230.11867933097074453No Hit
GTCCCTCTCCCAGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTG12010.11654446156652835No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT11900.11547702686442027No Hit
CCCTCATCCTCTTCCTGGACATTCTTAATGGTCAGGTTCCGAATCAGGCC11780.11431255264393872No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11780.11431255264393872No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTTAGT11490.1114984066111083No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11180.10849018154153098No Hit
CCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCCTATACTGACTCAGCCACCTGCTCTATCAGCCTCCCTGGGAGC11150.10819906298641059No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10930.10606419358219442No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10910.10587011454544748No Hit
CCTGCACCCTGAGGAGCGACTACAGTAATTATAGAGTGGACTGGCACCAG10800.1048026798433394No Hit
GTCCAGGAAGAGGATGAGGGTGACTACCACTGTGGGACAGACCTTAGCAG10730.10412340321472517No Hit
GTCTCCTCACAGGGTCCCTCTCCCAGCCTATACTGACTCAGCCACCTGCT10560.10247373140237631No Hit
GGTGGGATGTTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGCCTGATTCGGAAC10510.10198853381050899No Hit
CTATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGGAGCGACTACAGTAATTATAGAGTGGA10460.10150333621864167No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10400.10092109910840091No Hit
AGTGGGCACTGGTGGGATGTTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCAGTCTTGGGCTCAGGC10380.10072702007165397No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10330.10024182247978666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)