FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214842
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGACGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGGTACA56890.4682913498216229No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50940.4193137873073206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50190.4131401449735851No Hit
CCCTTGGACAGCCGGCCTCCATATCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGA47770.39321985904339823No Hit
GTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC39770.3273676741502187No Hit
GTGTATTACTGTGCGAGAGATCCGGCGTATAGTAGCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC39500.3251451629100739No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34050.28028336195159537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31970.26316179387936867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30900.2543540641499059No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30750.2531193356831588No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG29690.2443939211848125No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG29660.24414697549146308No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC29650.24406466026034662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28350.23336368021520493No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28220.23229358221069077No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28060.23097653851282718No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT27990.23040033189501186No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27100.22307427632564564No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26630.21920546046317133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26560.21862925384535603No Hit
GCTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG25950.21360802474725107No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC24200.19920285930186804No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA23730.19533404343939376No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23190.19088902095910415No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23100.19014818387905588No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATCAGGTTTCTAACCGGGAC23020.18948966203012407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22930.1887488249500758No Hit
CTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCC22690.18677325940328043No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22070.18166971507405902No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT21620.17796552967381765No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC21590.17771858398046822No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGATATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA20030.16487740792629824No Hit
CTCTGGGACCCCAGAGTCCCGGTTAGAAACCTGATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA19870.16356036422843465No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19690.1620786900683381No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATATCCTGCAGGTCTAGTCAAAGCCTCGTACA19490.16043238544600863No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19090.15713977620134964No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18810.15483494973008835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18730.15417642788115657No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18540.15261243848994355No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18120.1491551987830516No Hit
GTCCCAGAGAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGAA17920.14750889416072213No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17590.14479249153387846No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17280.14224071936926777No Hit
CCATATCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGC17110.1408413604402877No Hit
TCCATATCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGG16940.13944200151130764No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTC16810.13837190350679346No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATATCCTGCAGGTCTA16650.1370548598089299No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16610.13672559888446398No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16430.13524392472436744No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAG16390.13491466379990155No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC16380.1348323485687851No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16270.13392688102650388No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16230.13359762010203796No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT16210.13343298963980502No Hit
GTTTATTACTGCATGCAAGCTACACACTGGCCCACCTTCGGCCAAGGGAC16120.13269215255975675No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15870.1306342717818449No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15620.12857639100393303No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG15470.1273416625371859No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC15440.12709471684383647No Hit
GGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTACA15360.1264361949949047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15340.12627156453267174No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14910.12273200959466334No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA14670.12075644404786795No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14670.12075644404786795No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14670.12075644404786795No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG14600.12018023743005264No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14420.11869856326995609No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14220.1170522586476266No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14080.11589984541199595No Hit
GGATATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGACT13960.11491206263859828No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATATCCTGCAG13870.11417122555855No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13720.11293649709180288No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13700.11277186662956994No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13690.11268955139845345No Hit
GTCGTGTCCCTTGGCCGAAGGTGGGCCAGTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA13540.11145482293170636No Hit
GGCCTGGCCTCTGCTGAAACCAAGTCAAGTAGGTGTTTCCGTCACTGTGT13420.11046704015830866No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC13420.11046704015830866No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13400.11030240969607571No Hit
GCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGG13350.10989083354049332No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13220.10882073553597917No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13140.10816221368704737No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13080.10766832230034852No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13020.10717443091364967No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13000.10700980045141673No Hit
GGCCAATCTCCAAGGCGCCTAATTTATCAGGTTTCTAACCGGGACTCTGG12930.10643359383360142No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12920.10635127860248493No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12870.10593970244690257No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12790.10528118059797077No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12770.10511655013573781No Hit
GTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATCAGGTTT12750.10495191967350487No Hit
CAGTAATACACAGACGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGGTA12700.1045403435179225No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12680.10437571305568956No Hit
AGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG12540.10322329982005891No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12520.10305866935782595No Hit
GTTAGAAACCTGATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT12500.10289403889559301No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG12460.1025647779711271No Hit
AGCTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT12450.10248246274001063No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12430.1023178322777777No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12400.10207088658442827No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12390.1019885713533118No Hit
GGCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC12320.10141236473549646No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC12310.10133004950438No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTACACAC12200.10042458196209877No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG12150.10001300580651641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5150.054.2629240
CGTATGC5500.053.3834541
CGACGAT200.007204756652.02817544
TTATACA5800.051.7186282
TACACAT5850.051.276595
CTTATAC5900.050.869261
TATACAC5900.050.842043
TCGACGT5900.050.40536529
GTATGCC5700.050.34873642
CTTGAAA6000.050.20588757
AGACTCG6000.050.0461725
CTCGACG5950.049.95086328
GACGTCA5900.049.90632631
CCCACGA6050.049.58965719
ACTCGAC6000.049.5203127
CCGTCTT5700.049.5054847
CACGAGA6100.049.18723721
ACGAGAC6100.049.18723722
ATCTCGT4900.049.0941837
GACTCGA6150.048.84364326
Produced by FastQC (version 0.11.5)