FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214842
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52880.43528294214391666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52660.43347200705935424No Hit
GTAATACACAGACGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGGTACA51210.42153629854746544No Hit
GTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC42770.352062243485161No Hit
CCCTTGGACAGCCGGCCTCCATATCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGA41870.34465387268467834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41590.34234904621341705No Hit
GTGTATTACTGTGCGAGAGATCCGGCGTATAGTAGCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC38350.31567891133167936No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33070.27221646930218085No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32360.26637208789291117No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC29070.23929037685559107No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28820.2372324960776792No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG28720.23640934376651448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28620.23558619145534976No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27780.2286717120415659No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26350.21690063399191004No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26210.21574822075627942No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25960.21369033997836756No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24410.200931479155314No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23590.19418163020376314No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23420.19278227127478306No Hit
GCTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG22670.1866086289410475No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC22420.18455074816313563No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA22390.1843038024697862No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21860.17994109522061305No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.17969414952726362No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21500.17697774690041998No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATATCCTGCAGGTCTAGTCAAAGCCTCGTACA21200.17450828996692574No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21020.1730266158068292No Hit
CTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCC20950.17245040918901386No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20940.1723680939578974No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT20350.16751149532202542No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20280.1669352887042101No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATCAGGTTTCTAACCGGGAC20130.165700560237463No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19460.1601854397526592No Hit
CTCTGGGACCCCAGAGTCCCGGTTAGAAACCTGATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA19330.15911534174814504No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC18880.15541115634790367No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGATATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA18210.1498960358630999No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17780.1463564809250915No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17420.14339313260489842No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATATCCTGCAGGTCTA16750.13787801212009462No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16720.1376310664267452No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16690.1373841207333958No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTC16520.13598476180441574No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16490.1357378161110663No Hit
GTCCCAGAGAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGAA16440.1353262399554839No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16120.13269215255975675No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAG16010.13178668501747554No Hit
GTCAGTGCCTGACCCACTGCCGCTGAATCTCTCTGGGACCCCAGAGTCCCGGTTAGAAACCTGATAAATTAGGCG15890.13079890224407784No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15860.1305519565507284No Hit
TCCATATCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGG15850.13046964131961195No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15790.12997574993291308No Hit
GTTTATTACTGCATGCAAGCTACACACTGGCCCACCTTCGGCCAAGGGAC15670.12898796715951538No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15430.12701240161272No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15280.12577767314597288No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15230.12536609699039053No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15220.12528378175927404No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC15190.1250368360659246No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15160.1247898903725752No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG15120.1244606294481093No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14920.12281432482577981No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT14890.12256737913243039No Hit
CCATATCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGGC14700.12100338974121738No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14690.1209210745101009No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14300.11771078049655839No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14290.11762846526544193No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG13840.11392427986520058No Hit
GGCACTGACTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTACA13840.11392427986520058No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13770.11334807324738524No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13540.11145482293170636No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13460.11079630108277456No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTACACAC13400.11030240969607571No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13140.10816221368704737No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13090.10775063753146501No Hit
GTCGTGTCCCTTGGCCGAAGGTGGGCCAGTGTGTAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCA13040.10733906137588263No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13020.10717443091364967No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12960.10668053952695082No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12940.10651590906471788No Hit
GTGCCTGACCCACTGCCGCTGAATCTCTCTGGGACCCCAGAGTCCCGGTT12890.10610433290913551No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12800.10536349582908723No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12540.10322329982005891No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12520.10305866935782595No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12500.10289403889559301No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCC12500.10289403889559301No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATATCCTGCAG12480.10272940843336006No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12410.10215320181554473No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12390.1019885713533118No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12380.10190625612219531No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12330.10149467996661293No Hit
GCCTCGTACACAGTGACGGAAACACCTACTTGACTTGGTTTCAGCAGAGG12330.10149467996661293No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG12280.10108310381103057No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12280.10108310381103057No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCG12260.10091847334879762No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12220.10058921242433172No Hit
GTGTGAAGTCAGTGCCTGACCCACTGCCGCTGAATCTCTCTGGGACCCCA12210.10050689719321523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)