FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360471
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCGAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51500.37854537141916295No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48500.35649418473455147No Hit
GATTATTACTGCACCTCATATACAAACACTATCACTTATGTCTTCGGAACTGGGACCAGGGTCATCGTCCTAGGT44070.32393193239694196No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG40350.29658846090802377No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39840.29283975917163985No Hit
GACCTAGGACGATGACCCTGGTCCCAGTTCCGAAGACATAAGTGATAGTGTTTGTATATGAGGTGCAGTAATAAT36650.2693919973303363No Hit
CACTAGTGTGGCCTTGTTGGCTCGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35940.26417321648164493No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35360.2599099870559534No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG34890.2564553011420309No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACATT33100.24329809308687947No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31860.23418360259057341No Hit
GCCTTGGGCTGACCTAGGACGATGACCCTGGTCCCAGTTCCGAAGACATAAGTGATAGTGTTTGTATATGAGGTG30640.22521612000549807No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCACCTCATAT29760.21874777191134542No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29450.2164691492872689No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29310.2154400939086537No Hit
CTCCTGCACTGGAACCAGTAGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCGAAGC27530.20235638980911755No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAGCA27430.20162135025296388No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCGAGCCAACAAGG25780.18949319757642757No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25530.18765559868604328No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24850.18265732970419804No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACCCTGGTC24820.1824368178373519No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23520.17288130360735363No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22930.1685445702260467No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22440.16494287640089353No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21520.15818051248427933No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21420.15744547292812563No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20270.1489925180323579No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCGAGCCAACAAGGCC19750.1451703123403586No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19570.1438472411392819No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19440.14289168971628208No Hit
ACCTAGGACGATGACCCTGGTCCCAGTTCCGAAGACATAAGTGATAGTGT18640.13701137326705237No Hit
GTGATAGTGTTTGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGG18460.13568830206597568No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18380.13510027042105271No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18020.13245412801889933No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTCGCCTGGGTGCTGTTGGTACCAGGAGA17940.13186609637397637No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17180.12627979574720813No Hit
CACTATCACTTATGTCTTCGGAACTGGGACCAGGGTCATCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA16830.12370715730067013No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16260.11951743183059396No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16240.11937042391936323No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16060.11804735271828654No Hit
AGTGTGGCCTTGTTGGCTCGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15720.11554821822736391No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15640.11496018658244095No Hit
GTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGA15520.11407813911505649No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15120.1111379808904416No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14720.10819782266582675No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14610.10738927915405767No Hit
GGTACCAACAGCACCCAGGCGAAGCCCCCAAAGTCATGATTTATGAGGTC14590.10724227124282694No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14450.10621321586421174No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13970.1026850259946739No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGC13940.1024645141278278No Hit
GTACCAACAGCACCCAGGCGAAGCCCCCAAAGTCATGATTTATGAGGTCA13870.1019499864385202No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA13800.1014354587492126No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13800.1014354587492126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA200.00759788151.33409532
GGTATCA13600.049.6703951
TACGTAT851.2241617E-648.8804770
TCGTATG3100.047.79810340
CGTATGC3300.044.95299541
ACATGCG3550.044.24052427
ACGAGAC3700.042.4064222
CCCACGA3650.042.0512819
ATCTCGT3050.041.66514237
TCTCGTA3150.041.46807538
GATCTCG3150.040.31856536
GTCGTAT350.001362975739.33946239
CTTATAC4350.039.214991
AGTAGCG450.001435877838.92384769
CATGCGC4050.038.79443428
TCTCCGA4000.038.36897311
ATGCGCA4200.038.23904429
AATGGTA7250.038.20542570
ATGCCGT3550.038.02591744
ATCTCCG4150.037.8039710
Produced by FastQC (version 0.11.5)