FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360471
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43470.31952169506001965No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42920.3154789775011742No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG40230.2957064134406393No Hit
GATTATTACTGCACCTCATATACAAACACTATCACTTATGTCTTCGGAACTGGGACCAGGGTCATCGTCCTAGGT39600.2910756642368709No Hit
GACCTAGGACGATGACCCTGGTCCCAGTTCCGAAGACATAAGTGATAGTGTTTGTATATGAGGTGCAGTAATAAT35380.26005699496718415No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35190.2586604198104921No Hit
CACTAGTGTGGCCTTGTTGGCTCGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34580.25417667851795445No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33110.24337159704249484No Hit
GCCTTGGGCTGACCTAGGACGATGACCCTGGTCCCAGTTCCGAAGACATAAGTGATAGTGTTTGTATATGAGGTG31690.2329340353451121No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACATT30970.22764175054080538No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG30650.22528962396111346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30300.22271698551457547No Hit
CCTCTGAGGAGCTCCGAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28770.21147088030542363No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28750.2113238723941929No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC28270.20779568252465505No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAGCA27930.20529654803373246No Hit
CTCCTGCACTGGAACCAGTAGTGACATTGGTGATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCGAAGC27900.20507603616688633No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26720.1964025694042725No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26280.19316839535719615No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCACCTCATAT26080.19169831624488873No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACCCTGGTC24260.1783205963228911No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24120.1772915409442759No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23320.1714112244950462No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22830.16780953066989301No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22550.1657514199126626No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21910.16104716675327882No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21700.15950358368535603No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20020.14715491914197362No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19730.14502330442912786No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGACTTTGGGGGCTTCGCCTGGGTGCTGTTGGTACCAGGAGA18300.13451223877612972No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18160.13348318339751453No Hit
GTGATAGTGTTTGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGG17980.13216011219643783No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17890.1314985765958995No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17780.1306900330841304No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG17160.1261327878359774No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17090.1256182601466698No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCGAGCCAACAAGG17090.1256182601466698No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16190.11900290414128636No Hit
ACCTAGGACGATGACCCTGGTCCCAGTTCCGAAGACATAAGTGATAGTGT16160.11878239227444025No Hit
AGTGTGGCCTTGTTGGCTCGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16160.11878239227444025No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16030.11782684085144043No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15850.11650376965036374No Hit
CACTATCACTTATGTCTTCGGAACTGGGACCAGGGTCATCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA15550.11429865098190259No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15400.11319609164767203No Hit
GGCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC15090.1109174690235955No Hit
GTGTTTGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAG14540.10687475146475008No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14350.10547817630805802No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA14010.1029790418171354No Hit
GTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGA13860.1018764824829048No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCGAGCCAACAAGGCC13640.10025939545936664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)