FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775226
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36050.46502568283313506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32170.41497576190685037No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21400.2760485329439415No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20960.27037276871518756No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20630.2661159455436221No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18570.2395430493817287No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18370.2369631565504769No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17650.22767554235797044No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17000.21929089065640212No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16350.21090623895483382No Hit
GTCACAGTACGCCTCCGGCGTTCGGCCGAGGGACCAGGGTGGACATCAAA15720.20277957653639067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14720.18988011238013175No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14100.18188244460325118No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14050.18123747139543825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13990.1804635035460627No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13720.17698064822387277No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13620.1756907018082469No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.17478773931730876No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGACAAGTCAGTCCGTTAGTAATTATATCAATTGGTACCAACAAAAG13430.1732398036185577No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13100.16898298044699223No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12630.16292023229355054No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12330.15905039304667284No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12160.15685748414010883No Hit
TACTGTGACTGTGCTGACAGTAATAAGTTCCAAAATCTTCAGGTTGTAGACTACTGATGGTGAGAGTGAAAT11290.14563495032416354No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11010.14202310036041105No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10880.1403461700200974No Hit
AGTCTCCACCGTCCCTTTCTGCATCTGTAGGAGACAGAGTCACCGTCACT10670.137637282547283No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10610.13686331469790744No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10580.1364763307732197No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGTGATATCCGGGTGACCCAGTC10330.13325146473415495No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10130.13067157190290316No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10040.12951062012883985No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9860.12718871658071323No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9830.12680173265602548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9800.12641474873133768No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9650.12447982910789884No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9500.12254490948446002No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9430.12164194699352188No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9430.12164194699352188No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9420.1215129523519593No Hit
CTCCTGATCTATGAGGCATCCACTTTGCAGAGTGGAGTCTCATCAAGGTT9420.1215129523519593No Hit
GATTTCACTCTCACCATCAGTAGTCTACAACCTGAAGATTTTGGAACTTATTACTGTCAGCACAGTCACAGTACG9390.12112596842727152No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9310.12009401129477082No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATTCTCTGGATCCCT9310.12009401129477082No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9250.11932004344539528No Hit
CAGTACGCCTCCGGCGTTCGGCCGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT9080.11712713453883125No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCGGCCGAACGCCGGAGGCGTA9030.11648216133101831No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCGGCCGAACGCC8980.11583718812320536No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8850.11416025778289168No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8840.11403126314132911No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8770.11312830065039099No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8730.11261232208414063No Hit
GAACATAGAGGACTCGCCATGGAGTTTGGGCTGAACTGGGTTTTCCTTGT8710.11235433280101545No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGTGATAT8610.11106438638538954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8520.10990343461132625No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8440.10887147747882552No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8400.10835549891257518No Hit
GTGGAGTCTCATCAAGGTTCGGTGGCAGTGGATTTGGGACAGATTTCACT8370.1079685149878874No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8280.10680756321382409No Hit
GATCAGGACTTCTCACTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATTCTCTGGATC8240.10629158464757374No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8230.10616259000601116No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8160.10525962751507302No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7940.10242174540069605No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7860.10138978826819534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG200.00736200951.74305740
TAAGGTA200.007678237751.195585
TATCGCG352.3226301E-549.10336
CTATCGC352.3351522E-549.05837635
CCTATCG352.3432316E-549.02972834
GGTATCA7800.048.1357041
TATCTCG1250.046.74605636
CGTATGC1350.046.03885741
TCGTATG1400.044.3511940
GACCGGA2250.044.00731326
GCCTATC1750.043.1153933
GCCCACG2250.042.46515318
ACGAGAC2300.041.54735622
ACCGGAG2400.041.2781727
AGACCGG2400.041.2515325
ATCTCGT1450.040.3166637
GTGGTAT2100.039.0086751
TGGTATC2100.039.0061572
GAGACCG2500.038.23589724
AGCCTAT2350.037.9129332
Produced by FastQC (version 0.11.5)