FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775226
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32800.4231024243252935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28090.36234594814931387No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24350.31410195220490544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19970.2576022992004912No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18850.24315489934548118No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18490.23851109224922798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18460.2381241083245402No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17710.228449510207346No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16240.20948729789764536No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15790.2036825390273288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.20084465691295184No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15050.1941369355516972No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14970.19310497841919647No Hit
GTCACAGTACGCCTCCGGCGTTCGGCCGAGGGACCAGGGTGGACATCAAA13670.17633567501605985No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13580.17517472324199654No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13400.1728528196938699No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13380.17259483041074472No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13210.17040192150418074No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGACAAGTCAGTCCGTTAGTAATTATATCAATTGGTACCAACAAAAG12820.1653711304832397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12510.16137229659479946No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12370.1595663716129232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.14679590209822685No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10950.1412491325110355No Hit
TACTGTGACTGTGCTGACAGTAATAAGTTCCAAAATCTTCAGGTTGTAGACTACTGATGGTGAGAGTGAAAT10840.139830191453847No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10790.13918521824603405No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10750.1386692396797837No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10410.13428342186665568No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10400.13415442722509308No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10380.13389643794196787No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10360.1336384486588427No Hit
CTCCTGATCTATGAGGCATCCACTTTGCAGAGTGGAGTCTCATCAAGGTT10300.1328644808094672No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10020.12925263084571467No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9910.1278336897885262No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9850.12705972193915066No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCGGCCGAACGCC9330.120352000577896No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9250.11932004344539528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8900.11480523099070465No Hit
AGTCTCCACCGTCCCTTTCTGCATCTGTAGGAGACAGAGTCACCGTCACT8880.11454724170757948No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8880.11454724170757948No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGTGATATCCGGGTGACCCAGTC8670.11183835423476508No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8660.11170935959320251No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8650.11158036495163991No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATTCTCTGGATCCCT8630.11132237566851473No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCCTGGTCCCTCGGCCGAACGCCGGAGGCGTA8610.11106438638538954No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8580.11067740246070179No Hit
GATTTCACTCTCACCATCAGTAGTCTACAACCTGAAGATTTTGGAACTTATTACTGTCAGCACAGTCACAGTACG8560.1104194131775766No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8540.11016142389445142No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8360.10783952034632482No Hit
CAGTACGCCTCCGGCGTTCGGCCGAGGGACCAGGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT8350.10771052570476222No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8330.10745253642163705No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8300.10706555249694928No Hit
GATCAGGACTTCTCACTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATTCTCTGGATC8180.1055176167981982No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8010.10332470789163417No Hit
GTGGAGTCTCATCAAGGTTCGGTGGCAGTGGATTTGGGACAGATTTCACT7970.10280872932538383No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGTGATAT7940.10242174540069605No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7760.10009984185256945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC150.002299332869.4192945
CGGATCA400.007425079651.62658370
CTAAACA200.00774492851.0837064
GTGGTAT2000.047.7212221
GGTATCA7150.046.2433431
TGGTATC2150.044.3603252
GTATCAA19300.037.2656481
ATCAACG21000.033.893633
TATCAAC21350.033.6635362
TCAACGC21200.033.4132424
CAACGCA21550.032.7146455
AACGCAG22050.032.281736
GATTCAC1600.031.9561791
CACGCAT450.004486753630.85301645
ACGCAGA23250.030.7620647
AGAGTAC23600.030.45016111
CGCAGAG23500.030.4348098
GTGCTAT450.004925744630.2698712
GTACGTA600.00580215629.27161869
AACTGTA1454.964022E-528.48363170
Produced by FastQC (version 0.11.5)