FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096706
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAGTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTG54400.49603084144702414No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT44660.4072194371144135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42420.38679463776071255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT31300.28540009811198264No Hit
GTGTATTACTGTCAACAGTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTG30790.2807498089734167No Hit
TCTATGATGTGTCCAAAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC30240.2757347912749634No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27020.2463741422040182No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26930.2455535029442713No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26300.23980902812604288No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25640.23379100688789883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25430.23187618194848938No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25210.22987017486910802No Hit
GTAGTACACAGCCGAGTCCTCATCTCTCAGGCTGTCCATTTGCAGAAATA24910.22713471066995164No Hit
GTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCT24280.22139023585172327No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23440.21373093609408536No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC23350.21291029683433846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23100.2106307433350415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22470.20488626851681307No Hit
CAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22190.20233316859760045No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21310.19430914028007507No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGAACCAG21270.19394441172018753No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21170.19303259032046877No Hit
GAGTTATACTGTTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC20990.19139131180097493No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20760.18929412258162168No Hit
GTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20450.18646747624249344No Hit
CTCCTGCAGGGCCAGTCGGAGTTTTAACAACAACTACTTTGCCTGGTTCC20390.18592038340266215No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT19400.17689335154544608No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18630.16987232676761138No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTTTTAACAACAACTACTTTGCC18000.16412785194938295No Hit
GTGTACTACTGTGCGAGAGCGCTTAGTGAGAGTTGGTACGCTTTTGTCTGGGGCAAAGGGACCACGGTCATCG17890.1631248484096923No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATTTTGCAGTGTATTACTGTCAACAGTA17460.15920401639090148No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTTTTAACAA16470.15017698453368541No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTTTGGACACATCATAGATGAGGAGCC16400.14953870955388227No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15840.144432509715457No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15780.14388541687562573No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15730.14342950617576633No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15690.14306477761587882No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATTTTGCAGTGTATTACTGTCAACAGTATAACTCACCT15360.1400557669968068No Hit
GTATTATGGAGACTCTGTGAAGGGCCGCTTCACTATCTCCAGAGACAATTCCAAGGACACCGTATTTCTGCAAA15020.1369555742377629No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14750.13449365645852215No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTTTGGACACATCATAGA14570.13285237793902832No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14100.12856681736034997No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14070.12829327094043436No Hit
GTCCAAAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG13960.1272902674007437No Hit
CAGTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTGTGGCTGCACCA13750.1253754424613342No Hit
CATCTATGATGTGTCCAAAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13530.12336943538195286No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13470.12282234254212158No Hit
GTTATACTGTTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGC13310.12136342830257152No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13240.12072515332276837No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13110.11953978550313393No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12840.1170778677238932No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12800.11671313916400566No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12730.11607486418420251No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12560.11452476780468056No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA12530.11425122138476493No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12520.11416003924479305No Hit
GTTCCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTGTCCAAAAGGGCCACTGGCATCCCAGA12340.11251876072529922No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12300.1121540321654117No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTC12250.11169812146555229No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12120.11051275364591788No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGAACCAGGCAAAGTAGTTGTTGTTAAAACTCCGA12020.10960093224619907No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11940.10887147512642405No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11850.10805083586667712No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG11720.10686546804704268No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11670.10640955734718328No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11660.10631837520721141No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11660.10631837520721141No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGTTTGGTCC11610.10586246450735202No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11600.10577128236738013No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11510.10495064310763322No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11360.10358291100805504No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTGTCCAAAAGGGCCACTGGCATCCCAGACAGGT11170.1018504503485893No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11070.10093862894887053No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11030.10057390038898302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG8700.059.7726770
ATCGTAT309.563244E-657.04727639
ATATCGT252.6931497E-454.66699637
ACAGACG5600.050.98257470
TGTTAGA12000.046.73402470
TATCGTA306.5798895E-445.61256438
GGTATCA9700.043.9364361
TCGTATG3400.043.33681540
CGTATGC3450.042.7680241
TGCGTAG655.6579556E-542.2305169
ATCTCGT3050.040.32811437
TGCCGTC3500.039.365245
ACGAGAC4000.038.94920322
CCGTTCA350.001487108938.6472813
TATACAC4050.038.4122243
GTATCAA24850.038.111641
CACGAGA4100.037.98881521
GCTAATC3250.037.73859833
TCCGAGA7650.037.32057670
CTTATAC4200.037.045421
Produced by FastQC (version 0.11.5)