FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096706
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAACAGTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTGTG48870.4456071180425748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45570.4155170118518545No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43680.39828358739716935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA41590.3792265201430466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT28730.2619662881392096No Hit
TCTATGATGTGTCCAAAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT27430.25011260994286527No Hit
GTGTATTACTGTCAACAGTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTGTG27200.24801542072351207No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27170.24774187430359643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC25670.2340645533078145No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA23990.21874595379253875No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23960.2184724073726231No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23180.21136020045481652No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG22940.2091718290954914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22760.2075305505759976No Hit
GTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTT22670.20670991131625066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22530.20543336135664436No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC22160.2020596221776848No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22040.2009654364980223No Hit
GTAGTACACAGCCGAGTCCTCATCTCTCAGGCTGTCCATTTGCAGAAATACGGTGTCCTTGGAATTGTCTCTGGA21920.19987125081835969No Hit
CAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20870.19029712612131236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20550.18737929764221223No Hit
GAGTTATACTGTTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19900.18145245854404007No Hit
CTCCTGCAGGGCCAGTCGGAGTTTTAACAACAACTACTTTGCCTGGTTCCAACACAAACCTGGCCAGGCTCCCAG19470.17753162652524923No Hit
GTAATACACTGCAAAATCTGCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19220.17525207302595225No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGAACCAGGCAAAGTAGTTGTTGTTAAAACTCC19010.1733372480865428No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTTTTAACAACAACTACTTTGCCTG18630.16987232676761138No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18550.16914286964783634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA18180.16576913046887679No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17640.16084529491039531No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17290.15765392001137954No Hit
GTGTACTACTGTGCGAGAGCGCTTAGTGAGAGTTGGTACGCTTTTGTCTGGGGCAAAGGGACCACGGTCATCGT17270.15747155573143579No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATT16340.14899161671405098No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16340.14899161671405098No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15450.14087640625655373No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTTTTAACAA15320.1396910384369193No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTTTGGACACATCATAGATGAGGAGCCTG15210.1386880348972286No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14830.1352231135782972No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGCAGATTTTGCAGTGTA14590.13303474221897207No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTTTGGACACATCATA14380.13111991727956263No Hit
GTATTATGGAGACTCTGTGAAGGGCCGCTTCACTATCTCCAGAGACAATTCCAAGGACACCGTATTTCTGCAAAT13940.12710790312079992No Hit
GTCCAAAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCA13630.12428125678167166No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13490.12300470682206535No Hit
GTTCCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGTGTCCAAAAGGGCCACTGGCATCCCA13470.12282234254212158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13470.12282234254212158No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA13370.12191052114240279No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG13360.12181933900243092No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13280.12108988188265588No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13260.12090751760271212No Hit
CAGTATAACTCACCTTTCATTTTTGGCCAGGGGACCAAACTGGAGATCAGACGAACTGTGGCTGCACCAT13170.1200868783429652No Hit
CATCTATGATGTGTCCAAAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12730.11607486418420251No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT12480.11379531068490553No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGAACCAGGCAAAGTAGTTGTTGTTAAAACTCC12420.11324821784507424No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12340.11251876072529922No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12280.11197166788546793No Hit
GTTATACTGTTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGC12210.11133339290566478No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12200.1112422107656929No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12150.11078630006583351No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12110.110421571505946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12080.11014802508603035No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12060.10996566080608658No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11980.10923620368631155No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11940.10887147512642405No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTTTTGACGCAGTCTC11750.10713901446695834No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11450.10440355026780195No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA11190.10203281462853309No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11100.10121217536878616No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA11050.10075626466892677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCTA1504.1836756E-1179.9009570
TACGGAC150.002547722567.648372
AATGGTA2201.8189894E-1161.2876670
GTAGTTT1655.7796206E-754.4779270
TCCGGAA2103.0455722E-642.8040870
GTATCAA25400.040.3806231
GGGTCGA350.001384084839.2159541
GAGCAAA22750.038.85293670
AACCTTT2703.8329563E-738.8407470
GGGCGTA350.001487087138.64744
GGTATCA8950.037.4434851
CGCTTCA6800.035.2504270
ATCAACG30600.033.15343
TATCAAC31000.033.060412
CTCTTAA4108.227289E-932.88606370
TGGCGGT2751.9365798E-532.68675270
TCAACGC31100.032.4029244
GTGTTCA1850.003530801732.39227770
AAACACA19650.032.02137470
CCTGCTT7500.031.9603870
Produced by FastQC (version 0.11.5)