FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992374
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCGCAGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTGGCTGCACCAT38980.3927954581639584No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33330.3358612781068428No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT33150.3340474458218373No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29250.2947477463133859No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22670.2284420994504088No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20480.20637380664950913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT20140.20294767900005442No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTGGT19990.20143615209588323No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC18870.19015008454473817No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17700.17836017469220275No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT17610.1774532585497No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16970.17100407709190285No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTG16830.16959331864800972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16820.16949255018773166No Hit
CTATATACACTGGATGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTGGTG16030.16153184182576327No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGTGGATGCACCAAAGATGAGGAGCCTG15470.15588880805019076No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15400.1551834288282442No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA15370.15488112344740995No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTGTATTA15210.15326882808296066No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15200.1531680596226826No Hit
ACTATATACACTGGATGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGATCAACCCTAACAGTGGTG15130.15246268040073602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14880.149943468893784No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14770.14883501583072511No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCT14660.14772656276766621No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14650.14762579430738815No Hit
GTGTATTACTGTGCTACCAGTGGCTCGCTCCATTTCTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCCT14220.14329275051543067No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAAGGTCTCCTGCACGGCTTCTGGATACACCTTCACCG14150.14258737129348412No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13800.1390604751837513No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC13790.13895970672347321No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13720.13825432750152664No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAACTTAGCCTGGTA13650.13754894827958009No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13610.13714587443846774No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13570.13674280059735544No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13450.13553357907401847No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13320.1342235890904034No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTA13160.13261129372595412No Hit
GTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC12970.1306966929806706No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12760.1285805553148309No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGT12450.12545673304621038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12310.12404597460231727No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12290.12384443768176112No Hit
GTATTACTGTGCTACCAGTGGCTCGCTCCATTTCTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCCTCTC12180.12273598461870222No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCGCAGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAA11960.12051907849258445No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11950.12041831003230637No Hit
CCCTAACAGTGGTGGCACAAACTATGCACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACACGTCCATCA11920.12011600465147212No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11850.11941062542952556No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11850.11941062542952556No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAATTCCACCTTGGTCC11730.1182014039061886No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGTGGATGCACCAAA11510.11598449778007082No Hit
CTGTTAGGGTTGATCCGTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCATCCAGTGTATATAGTAGCCG11250.11336451781284072No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA11190.11275990705117223No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11130.11215529628950377No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTGATGGACGTGTCCCTG11060.11144991706755719No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10940.11024069554422021No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGG10760.10842686325921477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10650.10731841019615589No Hit
GTGTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCGTGCAGGAGACCTTCACCGAGGCCCCAGGCTTCTTCAC10370.10449689330836963No Hit
GTGTTAGTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGGTGCATCCA10350.10429535638781347No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA10330.10409381946725732No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10240.10318690332475457No Hit
TCTTTGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10110.10187691334113953No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10010.10086922873835873No Hit
GTCAGAGTGTTAGTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGGT9970.1004661548972464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAAT1204.4947228E-878.7907770
GTAGTTT1706.730261E-964.8865170
ACATTAT750.003367538863.0326170
AACTAGT1051.6926619E-460.0310670
TCGAGCA850.005527697555.61701270
GTTTGGT1551.8879155E-550.83275270
ATTGCAA851.0205986E-745.98784369
GGTATCA10100.044.5385861
GGGATGT3255.16593E-1043.63796270
AATGGTA2304.0186987E-641.10822770
GCGAGTA350.001388543539.18992642
GAGCAAA16400.038.4345270
AAGCGGT9900.038.20158470
GTATCAA24350.037.7812271
CGGCAAA1700.001819651937.07800770
TATTCTA2609.323883E-636.36496770
GCTGACA14200.035.5113370
TTCTTAA1850.002755311634.07168270
CTCCATT13100.033.68155370
GTATAAG851.4378384E-731.8329721
Produced by FastQC (version 0.11.5)