FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671501
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACATTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT48950.728963918147553No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41020.6108702742065909No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33620.5006693958758066No Hit
ATGTAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33420.49769099375875836No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA32960.4908406688895474No Hit
CTCCTAGGCTGCTCCTTTACTGGGCATCTTTCCGGCAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGTCTG29000.43186830697199263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22730.33849540060253075No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22390.3334321170035488No Hit
GCCTAGGAGGCTGTCCGGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGATAAAATATTCTGGCTG21980.3273263926635999No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20760.3091581397496057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20730.3087113794320485No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18570.27654463656792766No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17910.2667159095816685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16880.2513771386788702No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16740.2492922571969364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16570.2467606153974454No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16440.24482465402136405No Hit
GTTTATTACTGTCAGCAGTATTACATTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15640.2329110455531712No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGT15560.23171968470635187No Hit
ACATTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA15320.22814560216589402No Hit
GAGTAATGTAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT14960.22278447835520723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14860.2212952772966831No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14040.2090838286167854No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13900.20699894713485162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13790.20536082597047509No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13350.198808341312969No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTACATTACT12330.18361849051602305No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGAGTAAT12000.17870412702289348No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11540.17185380215368257No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11440.17036460109515844No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11290.16813079950737228No Hit
GTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT11250.16753511908396265No Hit
GTAATAAACGGCCGTGTCCGCGGCGGTCACAGAACTCAATTTCAGGGAGAACTGATTGTTGGACTTGTCCACTGA11150.16604591802543853No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11130.1657480778137337No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC10990.16366319633179996No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATCCACCAATAA10950.1630675159083903No Hit
GCTCCTTTACTGGGCATCTTTCCGGCAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTT10580.15755747199185108No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTGCCGGAAAGATGCCCAGTAAA10570.1574085518859987No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10540.15696179156844145No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10400.1548769100865077No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAGGCTGC10090.15026038680508294No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10020.14921794606411606No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9980.14862226564070644No Hit
CCTTTACTGGGCATCTTTCCGGCAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGTCTGGGACAGATTTCA9970.148473345534854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9650.14370790214757684No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT9580.14266546140660996No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATC9540.1420697809832003No Hit
GTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACCCCGGATTGCCGGAAAGATGCCCAGTAAAGGAGCAGCCTA9530.1419208608773479No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9520.1417719407714955No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9510.1416230206656431No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG9490.14132518045393827No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9450.14072950003052861No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC9410.14013381960711896No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9290.13834677833689002No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA9270.13804893812518523No Hit
GTAAAGGAGCAGCCTAGGAGGCTGTCCGGGTTTCTGCTGGTACCAAGCTA9270.13804893812518523No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9220.13730433759592317No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9110.13566621643154664No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8940.13313457463205564No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA8700.12956049209159776No Hit
GCCTCCTAGGCTGCTCCTTTACTGGGCATCTTTCCGGCAATCCGGGGTCCCTGACCGATTCACTGGCAGCGGGT8550.12732669050381162No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8280.12330584764579651No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGTCAGGGACC8270.12315692753994409No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8250.12285908732823927No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8080.12032744552874829No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGG7940.11824256404681453No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATATTTTATCCACCAATAAGAACTACTTA7860.11705120319999522No Hit
CTACTTAGCTTGGTACCAGCAGAAACCCGGACAGCCTCCTAGGCTGCTCCTTTACTGGGCATCTTTCCGGCAATC7860.11705120319999522No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7810.11630660267073319No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA7810.11630660267073319No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7780.11585984235317594No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTACATTACTCCTCGGACGTTCGGCCAAGGGACCAAGGT7740.11526416192976631No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7670.11422172118879942No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGG7470.1112433190717512No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7370.1097541180132271No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7370.1097541180132271No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT7340.10930735769566985No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATA7270.10826491695470296No Hit
GTCAGGGACCCCGGATTGCCGGAAAGATGCCCAGTAAAGGAGCAGCCTAGGAGGCTGTCCGGGTTTCTGCTGGTA7240.10781815663714574No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7060.10513759473180233No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTACATTACTCCTCGGACGTT7020.1045419143083927No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAG6970.10379731377913064No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTG6870.10230811272060653No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT6830.10171243229719687No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCA6770.10081891166208241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA800.005143276456.6310270
GACCCAT1952.7748683E-854.2108970
AATGGTA1401.3277753E-553.93430370
ATATAGG200.007800545550.99051735
ATAGTGC200.00814503350.4361466
GTGGTAT1450.046.4195481
GGTATCA6750.045.370811
GTATCAA18450.041.224081
TGGTATC1750.038.43042
GACAGGT1500.038.141391
ATGTGAA1600.001663556737.75401370
GAGCCAT6150.036.83318370
GAGCAAA17300.036.6628670
TATCAAC20850.036.28772
CGTCACA11100.035.37312770
ACCCAGT15450.035.18820670
CCGTGCA601.184376E-535.13367556
ATCAACG21650.034.7863123
CAACGCA21750.034.6289565
TCAACGC21950.034.313434
Produced by FastQC (version 0.11.5)