FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267466
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCTGCAGGGAGA62360.4920053082291754No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT45650.3601674522235705No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39720.3133811873454594No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36470.28773947387937826No Hit
GTATGAAAACCTCCCATTAACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTGTGGCTGCACCA36120.284978058583031No Hit
GTCTCTGGTGACTCCGTCGCCGGTGATCTTTACTACTGGAGCTGGGTCCGGCAGCCCGCCGGGAAGGGGCTGGAG34970.2759048368950331No Hit
CAGTAATAGACGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCTGCAGGGAGAACTGGTTTTTGGACGGGTCCA34240.27014531356265176No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG33270.2624922483127753No Hit
GTCTATTACTGTGCGCGAGGAGGTGGCTACAGCAGTAGTTGGTATCCTAATTTTCTCTACTGGGGCCAGGGAGC29060.2292763671767132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28500.2248581027025577No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC27870.21988755516913275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26580.20970976736259592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25800.20355575613073645No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25140.19834851585762459No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24290.19164222156649566No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24070.18990647480879172No Hit
CTATTACTGTGCGCGAGGAGGTGGCTACAGCAGTAGTTGGTATCCTAATTTTCTCTACTGGGGCCAGGGAGC23790.18769734257171397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23400.18462033695578423No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22810.1799653797419418No Hit
ATATTACTGTCAACAGTATGAAAACCTCCCATTAACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTG22630.1785452233038204No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22630.1785452233038204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22520.17767734992496842No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22310.17602050074716008No Hit
GTTTTCATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGGAGGCTGGTGATGGTGAAAGAAAAGTGTAA22060.17404806124976924No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20850.16450145408239747No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20750.16371247828344115No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20190.1592942138092856No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19800.15621720819335588No Hit
CACCTGCACTGTCTCTGGTGACTCCGTCGCCGGTGATCTTTACTACTGGA19680.15527043723460826No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19030.15014209454139205No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18690.14745957682494046No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18650.14714398650535793No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17980.14185784865235043No Hit
ACATATTACTGTCAACAGTATGAAAACCTCCCATTAACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAA17840.14075328253381156No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC17630.13909643335600325No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTTAATGGGAGGTT17580.13870194545652506No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG17440.1375973793379862No Hit
GTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGG17300.1364928132194473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17260.13617722289986478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16720.1319167535855005No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16140.12734069395155373No Hit
GTAATATGTTGCAAAATCTTCAGGCTGGAGGCTGGTGATGGTGAAAGAAAAGTGTAACCCGGATCCACTTCCA16040.1265517181525974No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGTACATTTACAACTATTTAAATTGGTATCAACAGAAA15980.12607833267322358No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15920.1256049471938498No Hit
GTGCAGCCACAGTTCGTTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTT15410.12158117061917242No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15320.12087109240011172No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15110.11921424322230341No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGTACATTTACAA15090.11905644806251214No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15040.11866196016303396No Hit
GGGTTACACTTTTCTTTCACCATCACCAGCCTCCAGCCTGAAGATTTTGC14990.11826747226355579No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGGAGGCTGGTGATGGTGAAAGAAAAGTGTAACCCGGATCCACTTCCA14920.11771518920428634No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14920.11771518920428634No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14890.11747849646459944No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14820.11692621340533No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14770.11653172550585182No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14690.11590054486668676No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAGCTCCCTGG14690.11590054486668676No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACA14570.11495377390793914No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG14530.1146381835883566No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14440.1139281053692959No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14290.11274464167086139No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14170.11179787071211378No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14150.11164007555232251No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG13980.11029881669409672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13900.10966763605493166No Hit
GTATCAACGCAGAGTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCT13850.10927314815545347No Hit
GTGTAACCCGGATCCACTTCCACTGAACCTTGATGGGACCCCTGTTTCCAATGTGGATGCATCGTAGATCAGGAG13820.10903645541576659No Hit
GTCCCTCACCTGCACTGTCTCTGGTGACTCCGTCGCCGGTGATCTTTACTACTGGAGCTGGGTCCGGCAGCCCGC13730.10832637719670588No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13670.10785299171733205No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC13660.10777409413743642No Hit
GGAGTGGATTGGGCGCATGTATCACAGTGGGAAGACCAATTACAACCCCT13610.10737960623795825No Hit
GGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGACTCCGTCGCCGGTGATCTTTA13470.10627504011941939No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13390.1056438594802543No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG13000.10256685386432457No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC12980.1024090587045333No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12940.10209346838495076No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12890.10169898048547259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTCG200.00823865450.29310613
CTCTTAT2350.045.6723751
GTGGTAT2150.043.6808471
TGTTAGA12100.043.5021470
TATACAC9350.043.0315363
ATCTCGT8750.042.80220837
CTTGAAA9800.042.76001757
AATCTCG8750.042.72860736
CGCTAAT9200.042.5980332
CACTGAA29200.042.5427170
TCGTATG9700.042.36465540
ATGCCGT9700.042.33916544
GCTAATC8850.042.0390133
TCGCTAA9500.041.8941431
CGTATGC9750.041.87040341
TTATACA9450.041.864922
ACACTCG9550.041.54454827
TGCTTGA10150.041.43457455
ACGAGAC9600.041.24169522
GTATGCC10000.041.23994442
Produced by FastQC (version 0.11.5)