FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267466
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92030.7260944277795223No Hit
GTAATAGACGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCTGCAGGGAGA55590.43859164663983097No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA38640.3048602487167309No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35030.2763782223744069No Hit
GTATGAAAACCTCCCATTAACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTGTGGCTGCACCAT32710.25807398383861974No Hit
CAGTAATAGACGGCCGCGTCTGCGGCGGTCACAGAGGTCAGCTGCAGGGAGAACTGGTTTTTGGACGGGTCCA32550.25681162256028955No Hit
GTCTCTGGTGACTCCGTCGCCGGTGATCTTTACTACTGGAGCTGGGTCCG31380.24758060571250037No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC29650.23393132439055564No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29550.23314234859159932No Hit
GTCTATTACTGTGCGCGAGGAGGTGGCTACAGCAGTAGTTGGTATCCTAATTTTCTCTACTGGGGCCAGGGAGCT28570.22541038576182715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC28220.22264897046547993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28050.22130771160725418No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT26010.20521260530854477No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24190.19085324576753934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA22720.1792553015228811No Hit
CTATTACTGTGCGCGAGGAGGTGGCTACAGCAGTAGTTGGTATCCTAATTTTCTCTACTGGGGCCAGGGAGCT21650.1708132604740482No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG21400.16884082097665737No Hit
ATATTACTGTCAACAGTATGAAAACCTCCCATTAACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAACTGT21230.1674995621184316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21230.1674995621184316No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC21170.16702617663905778No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20290.16008318960824194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20160.1590575210695987No Hit
GTTTTCATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGGAGGCTGGTGATGGTGAAAGAAAAGTGTAA19510.15392917837638248No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19390.15298240741763489No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19370.1528246122578436No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18910.14919532358264442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18830.14856414294347936No Hit
CACCTGCACTGTCTCTGGTGACTCCGTCGCCGGTGATCTTTACTACTGGAGCTGGGTCCGGCAGCCCGCCGGGAA18470.14572383006723652No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17750.14004320431475084No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17740.13996430673485522No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17700.13964871641527268No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGATCCACTTTGGTCC17190.13562493984059534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17140.13523045194111716No Hit
GTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTG17120.13507265678132588No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17040.13444147614216082No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16790.13246903664476994No Hit
GGGTTACACTTTTCTTTCACCATCACCAGCCTCCAGCCTGAAGATTTTGC16230.12805077217061445No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16130.1272617963716581No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGTACATTTACAA15990.12615723025311923No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGTACATTTACAACTATTTAAATTGGTATCAACAGAAA15960.12592053751343232No Hit
ACATATTACTGTCAACAGTATGAAAACCTCCCATTAACTTTCGGCCCTGGGACCAAAGTGGATCTCAAACGAA15900.1254471520340585No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15590.12300132705729384No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15510.12237014641812878No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT15290.12063439966042483No Hit
GTAATATGTTGCAAAATCTTCAGGCTGGAGGCTGGTGATGGTGAAAGAAAAGTGTAACCCGGATCCACTTCCA15260.12039770692073791No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14670.11574274970689549No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14640.11550605696720859No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14620.11534826180741732No Hit
GTGCAGCCACAGTTCGTTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTT14320.11298133441054829No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14220.11219235861159194No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAGCTCCCTGG14190.11195566587190506No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCA13940.10998322637451419No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC13810.10895755783587094No Hit
GTGTAACCCGGATCCACTTCCACTGAACCTTGATGGGACCCCTGTTTCCAATGTGGATGCATCGTAGATCAGGA13670.10785299171733205No Hit
GTATCAACGCAGAGTACGGGACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTT13670.10785299171733205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13480.106353937699315No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT13260.10461819094161105No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGGAGGCTGGTGATGGTGAAAGAAAAGTGTAACCCGGATCCACTTCCA13210.10422370304213288No Hit
GGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGACTCCGTCGCCGGTGATCTTTA13180.10398701030244599No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13010.10264575144422021No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG13010.10264575144422021No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12910.10185677564526385No Hit
GGAGTGGATTGGGCGCATGTATCACAGTGGGAAGACCAATTACAACCCCT12840.10130449258599442No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12810.10106779984630752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAA200.005955445153.6943270
CACTGAA26900.071.4192970
AAGTATT650.00210904570.9358470
AATGGTA3750.057.37920870
CTGGAGT17550.051.6693170
TATCCAA900.00683767651.23143470
GAGCAAA25150.043.9999670
GAGCCAT16400.041.23505870
TAGGCAA1500.001112680240.9851570
GTAGTTT1550.001307371739.66304470
GTGGTAT2800.039.470271
GAGGTTT15250.039.30542870
GATTCAC1650.038.564161
GTAGATA2009.858425E-538.4235870
TTATGAA1650.001777515737.25922870
AGACACA15850.036.84784770
CGGAGGT5251.8189894E-1235.13012770
GTCCACT15350.034.85481667
ACATCCA22450.034.23036270
CCGTCTA9900.034.1542970
Produced by FastQC (version 0.11.5)