FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994486
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30710.3088027382989806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28670.2882896290143853No Hit
GTATAAAAGTGCCTCGTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCA22360.22483976647232842No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22310.22433699418594127No Hit
CTTTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20400.2051310928459526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT20120.2023155680421846No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18340.18441687464680248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18290.18391410236041533No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17430.17526641903455653No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17420.17516586457727912No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16590.1668198446232526No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16300.16390376536220722No Hit
GATCTATTCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA16060.16149045838754894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15520.15606051769456786No Hit
GTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTG15500.155859408780013No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15070.1515355671170836No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCA14210.14288788379122483No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13920.1399718045301794No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG13870.13946903224379228No Hit
GAATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC13490.13564796286725No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAA13480.1355474084099726No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT13310.13383798263625632No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13280.13353631926442405No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAATGACGCCTGG13220.13293299252075946No Hit
AGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTAT13200.1327318836062046No Hit
AAACTATACGAGGCACTTTTATACTTTTGACAGTAATAAGTTGCAACATC13190.1326313291489272No Hit
GTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCCCCTGGCCAAAACTA12750.12820693302872035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12710.12780471519961065No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12590.12659806171228152No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT12580.12649750725500408No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATG12420.12488863593856525No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACA12380.12448641810945554No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTA12340.12408420028034582No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12260.1232797646221264No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12190.12257588342118442No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAATGACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12000.12066534873291328No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11670.11734705164275817No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11510.11573818032631933No Hit
GGCCAAAACTATACGAGGCACTTTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGA11190.11252043769344162No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11190.11252043769344162No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11090.11151489312066737No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGCGCTGGATTTTC11050.11111267529155765No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAAAAGTGC11020.11081101191972537No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10970.11030823963333823No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCTGATTGCAAAGTGGATGCAGAATAGA10950.11010713071878336No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGA10860.10920214060328652No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCCCCTGGCCAAAACTATACGAGGCACT10510.10568273459857654No Hit
ACTTATTACTGTCAAAAGTATAAAAGTGCCTCGTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAA10290.10347053653847316No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10200.1025655464229763No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10150.10206277413658915No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAA10140.10196221967931171No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10060.1011577840210923No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10010.10065501173470517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)