FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994486
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30990.31161826310274854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29700.29864673811396036No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA27670.2782341832866425No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21460.2157898653173599No Hit
GTATAAAAGTGCCTCGTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCAT20690.20804717210699797No Hit
CTTTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20440.2055333106750623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT20170.20281834032857177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC17920.1801935874411505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17730.1782830527528794No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17050.1714453496580143No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15730.1581721612973938No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15600.15686495335278727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14860.14942392351425762No Hit
GTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTG14580.14660839871048964No Hit
GATCTATTCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14100.14178178476117312No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14090.14168123030389568No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC14040.14117845801750853No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13800.13876515104285028No Hit
AGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATT13400.13474297275175318No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13210.13283243806348205No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT13170.13243022023437231No Hit
GTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCCCCTGGCCAAAACTA13100.13172633903343034No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT12660.12730194291322353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12570.12639695279772667No Hit
AAACTATACGAGGCACTTTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGT12560.12629639834044923No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG12420.12488863593856525No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT12350.12418475473762326No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAATGACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAG12310.12378253690851354No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGT12020.12086645764746815No Hit
GAATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAATAATTGCTAATGTCCTGACTC12010.12076590319019072No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11990.12056479427563586No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG11980.12046423981835844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11970.120363685361081No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG11780.11845315067280987No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCA11570.11634150706998389No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11470.11533596249720961No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAATGACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG11260.11322431889438363No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11140.1120176654070545No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTATCAGCAGAAA11000.11060990300517051No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT10900.10960435843239624No Hit
GGCCAAAACTATACGAGGCACTTTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCT10830.10890047723145424No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCTGATTGCAAAGTGGATGCAGAATA10820.1087999227741768No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10800.10859881385962196No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAAAAGTGC10540.10598439797040882No Hit
ACTTATTACTGTCAAAAGTATAAAAGTGCCTCGTATAGTTTTGGCCAGGGGACCAAGCTGGAGATCAATCGAA10420.1047777444830797No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATCTCCAGCTTGGTCC10310.103671645453028No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10210.10266610088025371No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGCGCTGGATTTTC10000.10055445727742773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT150.0036122473181.653670
CGCATAA750.00599248754.49607570
CAGCCAT9950.043.8159470
GAGCAAA17300.041.73832370
AATGGTA4950.041.2849170
GTATCAA23600.036.5217931
ATGACTT5100.034.72789470
TTTGACA14750.032.32818270
TATCAAC26900.031.9087282
CTGGTAT17950.031.8779370
TAGGCAA3901.2752935E-831.44004470
ATCAACG27250.031.2480893
TCAACGC27350.031.1354034
ACATCCA16650.031.09385770
AACGCAG27750.031.0490446
TAAGTCA2203.503558E-430.96367870
CAACGCA27600.030.8533785
ACTATCG553.85218E-430.4779268
AACTGTA2253.996759E-430.27559770
CGTCACA8200.029.90638270
Produced by FastQC (version 0.11.5)