FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663253
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATAGTTACCCCTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA19140.2885776619178504No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17420.26264487307256806No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC16700.25178928704431036No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16360.24666303808652204No Hit
CTATTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC16230.24470300172030884No Hit
CTCTTGTACAACTTCTGGATTCAAGCTTGGTGATTATACTCTGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG16100.24274296535409567No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15840.23882289262166922No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15160.22857039470609253No Hit
GTACAGAGGAGACTTCCACCACTGGGGCCAGGGCACCCTGGTCACCGTCT14460.21801635273417533No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT14410.21726249259332414No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14280.21530245622711094No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13830.20851771495944987No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13750.2073115387340879No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGG13090.19736058487485167No Hit
GTACCAGCCACCGTGACAGTAATAAATGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGCGACGCT12750.19223433591706332No Hit
CCTCTGTACCAGCCACCGTGACAGTAATAAATGGCTGTGTCCTCGGTTTT12470.18801271912829645No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11980.1806248897479544No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11740.17700636107186848No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACCTAAATCATTTCTAATGCCCTGACT11610.1750463247056553No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11210.16901544357884546No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT11070.16690463518446205No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11030.16630154707178105No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10930.1647938267900786No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG10840.16343687853654637No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10800.16283379042386542No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCAGAGTATAATCAC10800.16283379042386542No Hit
GGGCTGGAGTGGGTAGGTCTCATTAGAAGCAAAGCTAATGGTGGGACAAC10720.16162761419850344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10710.1614768421703332No Hit
ACAGAATACGCCGCGTCTGTGAAAGACAGATTCACCATCTCGAGAGATGA10640.16042143797314148No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10450.1575567694379068No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC10440.15740599740973654No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10410.1569536813252258No Hit
ACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAATGTGAGGTGCAGCTGG10370.15635059321254485No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG10280.15499364495901263No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10100.1522797484519482No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG10070.15182743236743745No Hit
GGGTAGGTCTCATTAGAAGCAAAGCTAATGGTGGGACAACAGAATACGCCGCGTCTGTGAAAGACAGATTCACCA9670.14579655124062763No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTGCCCTGG9480.14293188270539298No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT9460.14263033864905247No Hit
CCCTGAGACTCTCTTGTACAACTTCTGGATTCAAGCTTGGTGATTATACT9400.141725706480031No Hit
ATTATACTCTGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG9290.1400672141701583No Hit
GTCTGGGGGAGGCTTGGTACAGCCAGGGCGGTCCCTGAGACTCTCTTGTACAACTTCTGGATTCAAGCTTGGTGA9270.1397656701138178No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9120.13750408969126413No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9070.13675022955041288No Hit
GAGTATAATCACCAAGCTTGAATCCAGAAGTTGTACAAGAGAGTCTCAGGGACCGCCCTGGCTGTACCAAGCCTC9030.1361471414377319No Hit
GGCTGGTACAGAGGAGACTTCCACCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC9020.13599636940956167No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8970.13524250926871043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8940.13479019318419969No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8910.13433787709968895No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8870.13373478898700797No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGTGA8570.1292116281419006No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT8520.12845776800104938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8440.1272515917756874No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG8420.12695004771934693No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8400.12664850366300642No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGT8390.12649773163483619No Hit
GTGGTGGAAGTCTCCTCTGTACCAGCCACCGTGACAGTAATAAATGGCTGTGTCCTCGGTTTTCAGGCTGTTCAT8290.12499001135313373No Hit
GATCAGCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG8290.12499001135313373No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8210.12378383512777176No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8140.12272843093058003No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTGCCC8140.12272843093058003No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8140.12272843093058003No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8070.12167302673338831No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7990.12046685050802636No Hit
GTTTTCAGGCTGTTCATTTGCAGATAGGCGACGCTTTTGGAATCATCTCT7910.11926067428266439No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGTTGGTATCAGCAGAAA7880.11880835819815365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC7870.1186575861699834No Hit
GTAATAAATGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGG7630.11503905749389749No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTT7590.11443596938121653No Hit
GTGAAAGACAGATTCACCATCTCGAGAGATGATTCCAAAAGCGTCGCCTA7430.11202361693049259No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7410.1117220728741521No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTG7310.11021435259244963No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAG7270.10961126447976866No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT7230.10900817636708766No Hit
GAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAATG7200.10855586028257692No Hit
GTACAAGAGAGTCTCAGGGACCGCCCTGGCTGTACCAAGCCTCCCCCAGACTCCACCAGCTGCACCTCACAT7180.10825431622623644No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG7100.10704814000087448No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAA7020.10584196377551251No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6900.10403269943746957No Hit
GGTACAGCCAGGGCGGTCCCTGAGACTCTCTTGTACAACTTCTGGATTCAAGCTTGGTGATTATACTCTGACC6890.10388192740929932No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACA6870.10358038335295883No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTC6830.10297729524027784No Hit
GCCTGAAAACCGAGGACACAGCCATTTATTACTGTCACGGTGGCTGGTAC6780.10222343509942661No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6700.10101725887406464No Hit
GGAGTGGGTAGGTCTCATTAGAAGCAAAGCTAATGGTGGGACAACAGAATACGCCGCGTCTGTGAAAGACAGAT6700.10101725887406464No Hit
GTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6680.10071571481772416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2050.063.838870
TGTTAGA4200.057.5250770
GTGGTAT1300.057.0231361
TACGTAC252.8889716E-453.8924612
ATCTCGT1400.053.52327337
TCGTATG1800.051.31730740
ACGAGAC1800.050.55467222
CGTATGC1850.049.99543441
CTTGAAA1850.049.62779257
ATCTCCG1850.049.15495310
ATGCCGT1900.048.9160644
GTATGCC1900.048.7620242
TCTCCGA1900.047.86140411
GAGACCG1850.047.3844124
AGTAAGA855.449857E-447.3735970
CCGTCTT1900.047.32678647
CTTATAC1950.046.6552931
CGAGGCT1900.046.23515329
ATACACA2050.046.005764
TATACAC2000.045.4717643
Produced by FastQC (version 0.11.5)