FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663253
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20410.30772570949547157No Hit
ACATAATAGTTACCCCTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT17750.2676203500021862No Hit
CTATTATGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC16180.2439491415794576No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC15720.23701362828362632No Hit
CTCTTGTACAACTTCTGGATTCAAGCTTGGTGATTATACTCTGACCTGGGTCCGCCAGGCTCCAGGGAAGGG15480.2333950996075404No Hit
CCTCTGTACCAGCCACCGTGACAGTAATAAATGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATAGGC15310.23083197512864623No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14840.22374568980464468No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14640.22073024924123977No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13940.21017620726932257No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13670.20610536250872594No Hit
GTACAGAGGAGACTTCCACCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAA13560.20444687019885321No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC13220.1993206212410649No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA13120.1978129009593624No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12670.19102815969170137No Hit
GTACCAGCCACCGTGACAGTAATAAATGGCTGTGTCCTCGGTTTTCAGGC12310.18560036667757251No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12090.1822833820578271No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11770.17745867715637922No Hit
GGGCTGGAGTGGGTAGGTCTCATTAGAAGCAAAGCTAATGGTGGGACAAC11760.177307905128209No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11590.1747447806493148No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11530.17384014848029333No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11410.1720308841422504No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCAGAGTATAATCAC11320.17067393588871818No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11130.1678092673534835No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11060.1667538631562918No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10780.1625322463675249No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACCTAAATCATTTCTAATGCCCTGACTT10700.16132607014216294No Hit
ACAGAATACGCCGCGTCTGTGAAAGACAGATTCACCATCTCGAGAGATGA10040.1513751162829267No Hit
ACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAATGTGAGGTGCAGCTGGTG9990.1506212561420755No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC9820.14805813166318132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT9670.14579655124062763No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG9590.14459037501526567No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT9470.1427811106772227No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTTCCACTTGGT9220.13901180997296658No Hit
GTGGTGGAAGTCTCCTCTGTACCAGCCACCGTGACAGTAATAAATGGCTG9210.13886103794479632No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT9200.1387102659166261No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC9170.13825794983211537No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA9140.13780563374760463No Hit
GTCTGGGGGAGGCTTGGTACAGCCAGGGCGGTCCCTGAGACTCTCTTGTACAACTTCTGGATTCAAGCTTGGT9080.13690100157858315No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9080.13690100157858315No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG8940.13479019318419969No Hit
GGCTGGTACAGAGGAGACTTCCACCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGC8930.13463942115602945No Hit
GGGTAGGTCTCATTAGAAGCAAAGCTAATGGTGGGACAACAGAATACGCC8790.13252861276164601No Hit
CCCTGAGACTCTCTTGTACAACTTCTGGATTCAAGCTTGGTGATTATACTCTGACCTGGGTCCGCCAGGCTCCAG8760.13207629667713527No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCA8630.13011626031092208No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8590.12951317219824107No Hit
ATTATACTCTGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG8560.12906085611373036No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC8530.12860854002921962No Hit
GTTTTCAGGCTGTTCATTTGCAGATAGGCGACGCTTTTGGAATCATCTCTCGAGATGGTGAATCTGTCTTTCACA8470.12770390786019814No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8230.12408537918411225No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT8160.12302997498692052No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8130.12257765890240979No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC8040.12122071064887757No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT8010.12076839456436683No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTGCCCTGG7870.1186575861699834No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7740.1166975498037702No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGT7730.11654677777559996No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7560.11398365329670579No Hit
GATCAGCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGG7510.11322979315585456No Hit
GAGTATAATCACCAAGCTTGAATCCAGAAGTTGTACAAGAGAGTCTCAGGGACCGCCCTGGCTGTACCAAGCCTC7370.11111898476147111No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC7240.10915894839525792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC7210.10870663231074718No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG7200.10855586028257692No Hit
GAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAATGT7180.10825431622623644No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTGCCCTG7160.10795277216989596No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC7160.10795277216989596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7160.10795277216989596No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7050.10629427986002325No Hit
GTGAAAGACAGATTCACCATCTCGAGAGATGATTCCAAAAGCGTCGCCTATCTGCAAATGAACAGCCTGAAAACC7050.10629427986002325No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGTTT7040.106143507831853No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTC7000.10554041971917202No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTC6940.10463578755015054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6940.10463578755015054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC6930.1044850155219803No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA6860.10342961132478858No Hit
GGAGTGGGTAGGTCTCATTAGAAGCAAAGCTAATGGTGGGACAACAGAATACGCCGCGTCTGTGAAAGACAGATT6670.1005649427895539No Hit
GTACAAGAGAGTCTCAGGGACCGCCCTGGCTGTACCAAGCCTCCCCCAGACTCCACCAGCTGCACCTCACATTG6650.10026339873321342No Hit
GCCTGAAAACCGAGGACACAGCCATTTATTACTGTCACGGTGGCTGGTAC6650.10026339873321342No Hit
TCCCAGGAGTTTCCACTTGGTGATCAGCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTT6640.10011262670504316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCA150.002791111197.9795770
TACGTAT200.0065993047148.4846870
GTAGTTT652.1093154E-591.3751870
AATGGTA1955.456968E-1268.5313970
GACGCTT6900.064.55855670
TATTCTA750.004259394559.39386770
CTGCAAA7400.058.1899470
TTCCGCC252.7798137E-454.3143435
TTTAAGC200.007112275352.1956750
TGCTCTA250.00807596750.54343868
TAGATCT200.00816226650.409124
GTGGTAT1650.042.8230251
TCTTGTT2255.188791E-639.59591370
TAGAAAT6500.038.83445470
AAGCGGT6500.038.83445470
GCAATTA350.001528422338.43012617
GTTCATT10600.037.82156870
CTTTTAA7750.036.40269570
GTATCAA10200.036.2856181
GAGCCAT9550.035.76070870
Produced by FastQC (version 0.11.5)