FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653269
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT23310.35682085021637333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22630.34641166196467305No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT21610.3307978795871226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17000.2602297062925074No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT15270.23374750676979925No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13710.20986760431001625No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13510.2068060783536338No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13000.19899918716485857No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12800.19593766120847614No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12770.19547843231501877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12340.18889615150879654No Hit
GAGTTACGGTCCCCGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA12120.18552847295677585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11430.17496620840725643No Hit
GTAATAGACGGCCGTGTCTGCAGCGGTCACAGAGGGCACGTTCAGGGAGA10790.16516932534683262No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10770.16486317275119436No Hit
GTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10380.1588931971362486No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC9980.15277014522348376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9550.1461878644172615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT9410.1440447962477938No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA9400.14389171994997466No Hit
GTCTCAGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG9230.1412894228870496No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9000.13776866803720977No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8940.13685021025029506No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG8840.13531944727210385No Hit
GATCAGGAGTTTAGGGGGTTTCCCTGGTTTTTGCTGATACCAATTTAAAT8640.1322579213157214No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAT8460.1295025479549772No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8460.1295025479549772No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8420.1288902427637007No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8350.12781870867896686No Hit
GATTTCACTCTCACCATCAGCGGTCTGCACCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACGGTCCC8170.12506333531822267No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAAAAA8060.12337949604221232No Hit
ACTTACTACTGTCAACAGAGTTACGGTCCCCGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8040.12307334344657408No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7850.12016489378801076No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7700.11786874932072393No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC7560.11572568115125621No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7410.11342953668396938No Hit
GTCTCTGGTGGCTCCATTAATAATTACTACTGGAGTTGGATCCGGCAGCCCCCAGGAAAGGGACTGGAATGGAT7300.11174569740795903No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7260.11113339221668257No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7250.11098031591886344No Hit
CTGCTACTCTGGCTCCGAGGTGCCACATGTGACATCCAGATGACCCAGTC7220.11052108702540608No Hit
CCGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGGTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC7080.10837801885593837No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGA7040.10776571366466188No Hit
GCATAGATCAGGAGTTTAGGGGGTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTAATGGTCTGACT6950.10638802698428977No Hit
CAGTAATAGACGGCCGTGTCTGCAGCGGTCACAGAGGGCACGTTCAGGGAGAACTGGTTGTTGGACGTGTCCA6780.1037857299213647No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6710.10271419583663084No Hit
GTTTAGGGGGTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTAATGGTCTGACTTGCCCGGCAAGTGA6670.10210189064535435No Hit
CAGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT6650.10179573804971612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4450.048.60632740
CGTATGC4500.048.207941
CTCGTAT4300.047.76622439
CACTGAG6800.044.16457770
TATACAC4800.043.4341553
ATGCCGT5050.043.30588544
TGTTAGA6950.043.21138870
CACGAGA4850.043.06879421
GTATGCC5100.042.65188242
CCGTCTT5200.042.40051347
ACGAGAC4950.042.2084322
TTATACA4950.042.117972
TCTACAT4750.041.756632
CTTGAAA5400.041.5543957
CTGTTTA1000.001055376141.4233370
ATCTCGT4800.041.1265237
CTTATAC5200.040.7357031
CGAGCCC5000.040.38544515
TACACAT5350.039.590555
ATCTCCG5200.039.4596910
Produced by FastQC (version 0.11.5)