FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653269
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42590.6519519524116405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21700.3321755662674947No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21220.32482790397217687No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16390.25089205212554094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14680.22471600519847107No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14070.2153783510315046No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13670.20925529911873975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11980.18338540478730814No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11690.1789461921505536No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11030.16884315649449155No Hit
GAGTTACGGTCCCCGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10690.1636385623686414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10250.15690320526460003No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC10120.15491321339295144No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10020.15338245041476023No Hit
GTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9920.151851687436569No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9550.1461878644172615No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9470.14496325403470853No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT9150.14006481250449662No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8940.13685021025029506No Hit
GTAATAGACGGCCGTGTCTGCAGCGGTCACAGAGGGCACGTTCAGGGAGAACTGGTTGTTGGACGTGTCCACTGA8780.1344009894851891No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8740.1337886842939126No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCA8700.13317637910263613No Hit
GTCTCAGTCAAGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8670.13271715020917876No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA8670.13271715020917876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8650.1324109976135405No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8490.12996177684843457No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAAAAA8460.1295025479549772No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8300.12705332718987125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8220.12582871680731827No Hit
GATCAGGAGTTTAGGGGGTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGCTGCTAATGGTCTGACTTGCCCG7720.11817490191636218No Hit
GATTTCACTCTCACCATCAGCGGTCTGCACCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACGGTCCC7610.11649106264035183No Hit
ACTTACTACTGTCAACAGAGTTACGGTCCCCGACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA7570.11587875744907535No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7420.11358261298178851No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7340.11235800259923553No Hit
GTAGTAAGTTGCAAAATCTTCAGGGTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7170.10975570553631046No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATA7160.10960262923849133No Hit
CTGCTACTCTGGCTCCGAGGTGCCACATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATA7060.10807186626030012No Hit
GTCTCTGGTGGCTCCATTAATAATTACTACTGGAGTTGGATCCGGCAGCCCCCAGGAAAGGGACTGGAATGGATT6970.10669417957992802No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTCGGGGACC6860.10501034030391768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6620.10133650915625876No Hit
CAGTAATAGACGGCCGTGTCTGCAGCGGTCACAGAGGGCACGTTCAGGGAGAACTGGTTGTTGGACGTGTCCA6590.10087728026280139No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT6550.1002649750715249No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6540.10011189877370578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)