FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924745
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGAGACAGTGAGTTCTTGGAGTTGTCTC36810.3980556802145456No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGAGACA34520.37329209674018243No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28030.3031105872429697No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT27080.29283748492827755No Hit
GTATTACTGTGCGCGTCACGGGTCTTTTACGAGTACCAGCTACTACGACTACTGGGGCCAGGGCACCCTGCTCAC25340.27402148700452555No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20760.22449432005579917No Hit
GAGTTACAGAACCCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA20580.22254783751196275No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG20170.21811418282877984No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19630.2122747351972706No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA19040.20589459797025125No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT18790.2031911499927007No Hit
GTGTATTACTGTGCGCGTCACGGGTCTTTTACGAGTACCAGCTACTACGA18730.20254232247808854No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC18050.19518894397915101No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17690.1912959788914782No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17310.18718673796560134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16680.1803740490621739No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16520.17864384235654154No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16420.17756246316552132No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTATTACATGAGTTGGATCCGCCAGGCTCCAGGGAAGGGG16160.1747508772688687No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16040.17345322223964443No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16000.17302067056323636No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA15670.1694521192328696No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15500.1676137746081352No Hit
GCATAGATCAGGGACTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGGTCTGACT15320.1656672920642988No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC15090.1631801199249523No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14800.16004412027099363No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA14770.15971970651368755No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14740.15939529275638148No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14730.15928715483727948No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14550.15734067229344306No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC14460.15636743102152487No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14450.1562592931024228No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14260.1542046726394844No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14240.15398839680128035No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13920.1505279833900156No Hit
GATCAGGGACTTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATTTAAAT13530.14631060454503675No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAG13340.1442559840820983No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13150.14220136361915986No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG13060.1412281223472417No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG12890.13938977772250727No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGA12760.13798398477418097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12740.13776770893597695No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12730.13765957101687493No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12690.13722701934046683No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC12420.1343072955247122No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11980.12954922708422323No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11960.12933295124601918No Hit
GAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT11880.12846784789320298No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11770.12727833078308076No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11620.1256562619965504No Hit
GTCCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGA11340.12262840026169378No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11300.12219584858528566No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATGGGGGT11060.11960053852683712No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGAGCAGGGTGCCCTGGCCCCAGTAGTCGTAGTAGCTGGTA10990.11884357309312296No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10960.1185191593358169No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG10910.11797846974030679No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG10910.11797846974030679No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10810.11689709054928657No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG10700.1157075734391643No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGG10630.11495060800545015No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT10580.11440991840994004No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATAGA10450.11300412546161374No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10390.11235529794700161No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10390.11235529794700161No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG10330.11170647043238946No Hit
GGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGGTCTGACTTGCCCGGCAAGTGATGGTGAC10320.11159833251328745No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10290.11127391875598137No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGAACC10270.11105764291777732No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAG10260.11094950499867531No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA10120.109435574131247No Hit
GTCTGGGGGAGGCTTGGTCAAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGA10110.10932743621214498No Hit
CTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAACTCACTGTCTCTACAAATGAA10080.10900302245483891No Hit
ACTTACTACTGTCAACAGAGTTACAGAACCCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA9940.1074890915874106No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9930.10738095366830856No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG9850.10651585031549238No Hit
GTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTT9720.10511005736716608No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC9630.10413681609524787No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9590.10370426441883979No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9580.10359612649973778No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG9550.1032717127424317No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9550.1032717127424317No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT9550.1032717127424317No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9530.10305543690422765No Hit
CTCCAGGCTTGACCAAGCCTCCCCCAGACTCCACCAGCTGCACCTGACAC9520.10294729898512563No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9460.10229847147051349No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTATTAC9460.10229847147051349No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT9310.10067640268398316No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAGCTATTTAAATTGGTA9300.10056826476488112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCG200.001139271782.85467569
GTTATTA701.9622913E-458.27103470
TTTGCGG550.00552791555.62234570
AGGTACG252.8425912E-454.0716322
TCTGGAG14750.045.6291870
GTGGTAT1750.044.4328041
AATGGGG958.852033E-442.93654670
GACAGGT2200.041.49111
CTCTTAT903.6379788E-1241.3203621
CGTCAGA751.0659278E-940.555925
TCGTATG2650.038.76761240
TCGCTTT350.001491664138.62259711
GACCGTA2650.038.30794526
ACGAGAC2750.038.118622
CCGTCTT2750.037.6778747
CGTCTTC2850.037.63216848
GTATGCC2750.037.4358342
TGTTAGA8500.037.19062870
TACACAT3000.037.1762585
AATGGTA1658.167797E-637.08156670
Produced by FastQC (version 0.11.5)