FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079559
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCATGTCTTCAACTCTCAGGCTGCCCACTTGAAAATACA39250.36357438546665816No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGCCAGTGCCCCAGGTCTGACAGTAA36490.33800839046314285No Hit
CAGTAATACACAGCCATGTCTTCAACTCTCAGGCTGCCCACTTGAAAATACAGCGTGTTCTTGGAATTGTCTC36070.33411791296260784No Hit
GTGTATTACTGTGCCCGGTCTTTTTGTAGTAGTAGTGTTAGCTGCTACCA34970.3239285671278735No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG33580.31105293920943644No Hit
GGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCAC30960.2867837700394328No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30060.27844703253828645No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCC29420.27251868587080463No Hit
GTATTACTGTGCCCGGTCTTTTTGTAGTAGTAGTGTTAGCTGCTACCACG29130.26983240378710194No Hit
GGATATAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGACAAGGG28250.26168092711931445No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT24660.22842660753140867No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24420.22620347753110298No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGATGAGGTAGC24130.22351719544740026No Hit
GTATGTGTTATGCCCATCACGACTAATACCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAG23400.21675517502980382No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22240.20601004669499307No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21720.20119326502766408No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTC21600.20008170002751122No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGACCTCGTCCCCCTTCCT21260.19693226586041152No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20830.19294915794319717No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG20510.18998498460945626No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCGAGGTGTTCGGCGGAGGGAC20470.18961446294273865No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20390.18887341960930343No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG19380.1795177475246837No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAA18850.1746083354406753No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18680.17303361835712547No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT18420.170625227523461No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGC18230.1688652496065523No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18190.1684947279398347No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18120.16784631502307887No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18100.16766105418972005No Hit
GCACTGAACACAGAAGAGTCATGACGGACTTTGGGCTGAGCTGGGTTTTC18080.16747579335636126No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17610.1631221637724293No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17540.16247375085567348No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17370.1608990337721236No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17280.160065360022009No Hit
GTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACCTGCACTCTGAGCAGTGGATATAGCAGCTACGCCATCGCA17190.15923168627189435No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16950.1570085562715887No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16940.1569159258549093No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16720.1548780566879624No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGG16660.15432227418788597No Hit
TAACACATACTATGCAAACTCTGTGAAGGGCAGATTCACCGTCTCCAGAGACAATTCCAAGAACACGCTGTATTT16570.15348860043777135No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC16400.1519138833542215No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16160.14969075335391582No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGATGAGGTAGCGCTCAGCCCCAGAGCTGGAGC15920.14746762335361013No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15400.14265084168628117No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15360.14228032001956356No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15340.14209505918620474No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15340.14209505918620474No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC15280.1415392766861283No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15250.1412613854360901No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15240.1411687550194107No Hit
CTCCTGTGCAACCTCTGGATTCACCTTCAGTAGTTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA15180.14061297251933427No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15060.13950140751918144No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14900.13801932085231097No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14840.13746353835223457No Hit
CTATTACTGTCAGACCTGGGGCACTGGCGAGGTGTTCGGCGGAGGGACCA14830.13737090793555518No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14770.13681512543547875No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14760.13672249501879935No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14530.13459199543517306No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT14480.13412884335177605No Hit
GCTATATCCACTGCTCAGAGTGCAGGTGAGTTTGACCGAGGCTCCCAGGGAGGCAGAGACAGAGGGCGATTGAG14340.1328320175182644No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGCCAGTGCCCC14290.1323688654348674No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14250.13199834376814976No Hit
GCTCTGGGGCTGAGCGCTACCTCATCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGTCAGAC14230.13181308293479096No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA14180.13134993085139396No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCCGCCCTGGGATTCCCAGGTGTTTTCATTTGGTGA13820.12801523585093544No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAAC13610.12606999710066796No Hit
CTGTTGAGTCTCATCAAGTAACGAGGGCCCTTGTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTATA13550.12551421460059153No Hit
GGGTTCCCTGGCCCCAGTAGTCAATCTCGCCGTGGTAGCAGCTAACACTA13520.12523632335055332No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCC13490.12495843210051513No Hit
CACCTGCACTCTGAGCAGTGGATATAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGACAAGGGCCCTCG13400.12412475835040049No Hit
GTGGATATAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGACAAG13390.12403212793372109No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG13330.12347634543364465No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13260.12282793251688884No Hit
CCTGCACTCTGAGCAGTGGATATAGCAGCTACGCCATCGCATGGCATCAG13200.12227215001681241No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT13020.12060480251658316No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG12970.12014165043318616No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12970.12014165043318616No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCCGCCCTGGGATTCCCAGGTGT12550.11625117293265122No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12530.11606591209929239No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12490.11569539043257478No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCCGCCCTGGGATTCCCAGGTGTTT12250.1134722604322691No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCAC12250.1134722604322691No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCC12250.1134722604322691No Hit
GAACACAGAAGAGTCATGACGGACTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTTAAAGGTGTCCAGTG12250.1134722604322691No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTC12200.1130091083488721No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12110.11217543459875745No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAACCTCTGGATTCACCTTCAGTAG12090.11199017376539865No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG12030.11143439126532223No Hit
CCCTGAGACTCTCCTGTGCAACCTCTGGATTCACCTTCAGTAGTTATGCT12000.11115650001528402No Hit
CTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACCTGCAC12000.11115650001528402No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11930.1105080870985282No Hit
GATCAGCACTGAACACAGAAGAGTCATGACGGACTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTTAAAGG11900.11023019584848999No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11790.10921126126501655No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC11720.10856284834826072No Hit
ATGCAAACTCTGTGAAGGGCAGATTCACCGTCTCCAGAGACAATTCCAAG11680.1081923266815431No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG11650.1079144354315049No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11580.10726602251474908No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11520.10671024001467266No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11340.10504289251444339No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT11200.10374606668093174No Hit
GTAGTTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTCAGGTATTAGTCGTGA11040.1022639800140613No Hit
GTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACC10890.10087452376387025No Hit
GTCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCATCATCTCCAGCCTCCAGTCTGACGA10800.10004085001375561No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG10800.10004085001375561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA150.008271705137.7054970
AGGGGGG1700.078.9781570
TCTGGAG13900.054.24011270
AATGGTA1757.4578566E-1153.11497570
CGGTACC408.4801286E-751.5575645
GTCCCGT200.00771798651.13012311
GTGGTAT1750.048.708891
CCGCATT306.694319E-445.4532121
GGTATCA6250.045.2797851
ATCTCGT1600.044.9282237
CTAGATC1550.044.0952333
TCTCGTA1650.043.58905838
TGGTATC1800.043.557312
TCGTATG1750.043.09341440
AAACGGG358.8604924E-442.93762267
CGTATGC1700.042.35827641
ACGAGAC1800.041.66737422
CGTACTA1650.041.3651729
GACCGTA1650.041.3325926
TTAACGG504.083544E-641.03523635
Produced by FastQC (version 0.11.5)