FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079559
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCATGTCTTCAACTCTCAGGCTGCCCACTTGAAAATACAGCGTGTTCTTGGAATTGTCTCTGGA35780.3314316308789052No Hit
GGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCAC33900.3140171125431774No Hit
GTGTATTACTGTGCCCGGTCTTTTTGTAGTAGTAGTGTTAGCTGCTACCACGGCGAGATTGACTACTGGGGCCAG32960.3053098533753134No Hit
CAGTAATACACAGCCATGTCTTCAACTCTCAGGCTGCCCACTTGAAAATACAGCGTGTTCTTGGAATTGTCTCTG32450.3005857021246639No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT32140.29771415920760236No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTC31520.2919710733734794No Hit
GTATTACTGTGCCCGGTCTTTTTGTAGTAGTAGTGTTAGCTGCTACCACGGCGAGATTGACTACTGGGGCCAGG29640.27455655503775156No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28920.26788716503683446No Hit
GGATATAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGACAAGGG26630.24667479961725114No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCT25770.23870858378282245No Hit
GTATGTGTTATGCCCATCACGACTAATACCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGT21810.20202693877777872No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGATGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGA21780.2017490475277405No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21080.19526491836018225No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG20710.19183759294304434No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20560.1904481366928533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20070.18590924627556252No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT19680.18229666002506578No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19540.18099983419155413No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19460.18025879085811894No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCGAGGTGTTCGGCGGAGGGAC19430.1799808996080807No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC19190.17775776960777503No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGACCTCGTCCCCCTTCCT18630.17257046627372843No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG18460.17099574919017857No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18430.17071785794014038No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG18350.16997681460670513No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18150.16812420627311708No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGC18130.1679389454397583No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA17850.16534529377273496No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAA17580.1628442725223911No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17490.16201059877227647No Hit
GTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACCTGCACTCTGAGCAG17410.16126955543884122No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17260.15988009918865018No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16830.15589699127143583No Hit
GCACTGAACACAGAAGAGTCATGACGGACTTTGGGCTGAGCTGGGTTTTC16810.15571173043807704No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16730.1549706871046418No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16720.1548780566879624No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG16680.15450753502124479No Hit
TAACACATACTATGCAAACTCTGTGAAGGGCAGATTCACCGTCTCCAGAG16360.15154336168750387No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA16290.15089494877074805No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG16090.14904234043715997No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16040.14857918835376296No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG15970.14793077543700714No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15830.1466339496034955No Hit
CTCCTGTGCAACCTCTGGATTCACCTTCAGTAGTTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGG15750.1458929062700603No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15470.143299254603037No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGT15050.13940877710250205No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA14870.13774142960227279No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGATGAGGTAGCGCTCAGCCCCAGAGCTGGAGC14780.13690775585215814No Hit
CTATTACTGTCAGACCTGGGGCACTGGCGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAA14590.13514777793524949No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC14060.1302383658512411No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG14040.1300531050178823No Hit
GGGTTCCCTGGCCCCAGTAGTCAATCTCGCCGTGGTAGCAGCTAACACTA14020.12986784418452352No Hit
CACCTGCACTCTGAGCAGTGGATATAGCAGCTACGCCATCGCATGGCATC13670.1266257796007444No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG13620.12616262751734736No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13540.12542158418391214No Hit
GCTCTGGGGCTGAGCGCTACCTCATCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTACTGTCAGAC13380.12393949751704168No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT13340.12356897585032406No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT13270.12292056293356825No Hit
GTGGATATAGCAGCTACGCCATCGCATGGCATCAGCAGCAGCCAGACAAG13240.12264267168353005No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13210.12236478043349183No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT13080.12116058501665959No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG13010.12051217209990375No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA13000.12041954168322436No Hit
GCTATATCCACTGCTCAGAGTGCAGGTGAGTTTGACCGAGGCTCCCAGGGAGGCAGAGACAGAGGGCGATTGAGT12930.11977112876646852No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT12770.11828904209959808No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12710.11773325959952166No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT12690.11754799876616286No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12540.1161585425159718No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT12520.11597328168261299No Hit
CTGTTGAGTCTCATCAAGTAACGAGGGCCCTTGTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGCTGCTATAT12500.11578802084925419No Hit
ATGCAAACTCTGTGAAGGGCAGATTCACCGTCTCCAGAGACAATTCCAAGAACACGCTGTATTTTCAAGTGGGCA12430.11513960793249838No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCT12420.11504697751581897No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACT12370.11458382543242193No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG12320.11412067334902493No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCCGCCCTGGGATTCCCAGGTGTTTTCATTTGGT12280.11375015168230733No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGCCAGTGCCCC12190.11291647793219269No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAAC12170.11273121709883388No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12140.11245332584879565No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTG12050.11161965209868104No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCCCGCCCTGGGATTCCCAGGTGT12000.11115650001528402No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11930.1105080870985282No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11900.11023019584848999No Hit
CCTGCACTCTGAGCAGTGGATATAGCAGCTACGCCATCGCATGGCATCAG11710.10847021793158132No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG11530.10680287043135206No Hit
AACACATACTATGCAAACTCTGTGAAGGGCAGATTCACCGTCTCCAGAGACAATTCCAAGAACACGCTGTATTTT11510.10661760959799327No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC11480.10633971834795504No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCT11310.10476500126440519No Hit
CTCCCAGCTTGTGGTGACTCAATCGCCCTCTGTCTCTGCCTCCCTGGGAGCCTCGGTCAAACTCACCTGCACT11300.10467237084772578No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC11250.10420921876432877No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC11240.10411658834764938No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11240.10411658834764938No Hit
GTAGTTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTCAGGTATTAGTCGTGATG11170.10346817543089354No Hit
GATCAGCACTGAACACAGAAGAGTCATGACGGACTTTGGGCTGAGCTGGG11150.10328291459753473No Hit
GAACACAGAAGAGTCATGACGGACTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTTAAAGGTGTCCAGTGT10830.10031874126379384No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCCCGCCCTGGGATTCCCAGGTGTTT10810.10013348043043502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT303.9722363E-7203.0939270
AATGGTA2005.456968E-1268.54420570
TAGGCAA909.341417E-567.69797570
GTACGTA403.5792127E-667.3241869
CTGCGTT700.00293147665.2801970
CTGTTTA700.00293147665.2801970
ATGGCAA1452.1099368E-763.0291570
CTCAACA19500.053.89800370
GTGGTAT1150.053.303991
AACGCTA200.00700736452.39455461
CCTACGT200.0072111252.0161556
GAGCCAT14600.049.03466470
CATTCTT950.00981524948.10119270
GGTATCA5600.046.8260771
GGTAGAA21100.045.47956570
CGTGGTA12000.040.61878670
GTATTTT15300.039.82234270
GTATCAA14750.039.481091
CACAGTA13150.039.38323270
GACAGGT1900.037.6400951
Produced by FastQC (version 0.11.5)