FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736229
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31640.42975758901102784No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30690.41685399515639837No Hit
CTACAAACTCTGATCACCTTCGGCCAAGGGACACGACTAGAGATTAAGCG23760.3227256736694697No Hit
CCCCTGGAGAGCCGGCCTCCATATCTTGCAGGTCAAGTCAGAGCCTCCTGCACAATAATGGATACTACTCTT23150.3144402081417602No Hit
GAGTTTGTAGAGATTGCATGCAATAATAAAGGCCAACATCCTCAGCCTCCACTCTGGTGATTGTCAGCGTAAAA22480.30533977879165314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT21820.29637517674527897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21630.2937944579743531No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21540.2925720122407566No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG21270.2889046750399672No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAACATG19900.27029633442855416No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC19550.2655423787979012No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19480.2645915876717706No Hit
TTATTATTGCATGCAATCTCTACAAACTCTGATCACCTTCGGCCAAGGGACACGACTAGAGATTAAGCGAACTG19360.2629616600269753No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19000.2580718770925894No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18700.2539970579806011No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC18410.2500580661723458No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17890.24299504637823288No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17380.23606785388785284No Hit
GTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT16450.22343591464068924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15730.21365634877191741No Hit
GTGCTAATGGAGCGGCTGTGGCCTGGATCCGTCAGCCCCCCGGAAAGGCCCTGGAATGGCTCGCACTCATTTCT14670.1992586545762256No Hit
ATAATGGATACTACTCTTTGGATTGGTATGTGAAGAAGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGT14580.19803620884262912No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14290.19409721703437383No Hit
GTGCAGCCACAGTTCGCTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTG13530.18377434195067024No Hit
CTCCAGGGGTGACGGCCAGGGAAAGTGGAGACTGAGTCAGCACTATTTCCCCACTGAATCCAGAGACCCAGAGCA13330.18105779587601142No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13270.18024283205361374No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13230.17969952283868199No Hit
GTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13160.1787487317125514No Hit
CATGAAGACAGATGGTGCAGCCACAGTTCGCTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTGATCAGAGTTTG12980.17630384024535845No Hit
CCTTCGGCCAAGGGACACGACTAGAGATTAAGCGAACTGTGGCTGCACCA12710.172636503044569No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCTGA12360.16788254741391606No Hit
GCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCTGACTCAGTCTCCACTTTCCCTGGCCGTCACCC12200.16570931055418897No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12070.16394355560566073No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12020.16326441908699604No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCTGACTCAGTCTCCACTTTCC11980.16272110987206428No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11960.1624494552645984No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11880.16136283683473485No Hit
GAGTAGTATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAAGATAT11660.1583746361526101No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11240.15266988939582657No Hit
CAATAATAAAGGCCAACATCCTCAGCCTCCACTCTGGTGATTGTCAGCGTAAAATCTGTGCCTGACCCACTGCCC11200.15212658018089478No Hit
GACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTACAAACTCTGATCACCT11180.15185492557342892No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11060.1502249979286336No Hit
CGACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAA10990.14927420680250303No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10900.14805176106890655No Hit
ATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAAGATATGGAGGCC10740.14587852420917946No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10720.1456068696017136No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10420.14153205048972534No Hit
GAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTACAAACTCTGATCACCTTCGGCCAAGGGA10080.13691392216280532No Hit
GATTTTACGCTGACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTACAAAC10020.13609895834040767No Hit
GTAATATGTGGCCGTGTCCACAGGGTCCACGTCGGCCAGTCTAAGCAGAACCTGGTTTTTGGAAGTGTCCTTGG9990.13569147642920884No Hit
GTTGTGGAGACTGCCCTGGCTTCTTCACATACCAATCCAAAGAGTAGTATCCATTATTGTGCAGGAGGCTCTGA9970.13541982182174297No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCTGACTCAGTCTCCACTT9950.13514816721427708No Hit
ACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAATC9920.13474068530307826No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9860.1339257214806806No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9830.13351823956948178No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9770.13270327574708413No Hit
GGACACGACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATG9620.13066586619109No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG9580.13012255697615824No Hit
GTATGTGAAGAAGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGTTCAAATCGGGCCCTCGGGGTCCCTGA9500.1290359385462947No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9440.12822097472389704No Hit
GCGTAAAATCTGTGCCTGACCCACTGCCCCTGAATTTGTCAGGGACCCCGAGGGCCCGATTTGAACTCAAATATA9090.12346701909324409No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9060.12305953718204526No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9040.12278788257457937No Hit
CTCCACAACTCCTAATATATTTGAGTTCAAATCGGGCCCTCGGGGTCCCT9010.12238040066338055No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8900.12088630032231819No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8870.12047881841111936No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8860.12034299110738642No Hit
GGTGATTGTCAGCGTAAAATCTGTGCCTGACCCACTGCCCCTGAATTTGT8790.11939219998125584No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG8730.11857723615885818No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8700.11816975424765935No Hit
GGGGAAATAGTGCTGACTCAGTCTCCACTTTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATATCTTGCAGG8690.11803392694392642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8690.11803392694392642No Hit
ATACTACTCTTTGGATTGGTATGTGAAGAAGCCAGGGCAGTCTCCACAAC8620.11708313581779582No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT8590.116675653906597No Hit
GCCCTGGCTTCTTCACATACCAATCCAAAGAGTAGTATCCATTATTGTGC8590.116675653906597No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8580.11653982660286405No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8550.11613234469166522No Hit
AGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGTTCAAATCGGGCC8480.11518155356553463No Hit
CTTCACATACCAATCCAAAGAGTAGTATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAAGATATGGAGGC8460.11490989895806875No Hit
GGTCAAGTCAGAGCCTCCTGCACAATAATGGATACTACTCTTTGGATTGGTATGTGAAGAAGCCAGGGCAGTCTC8430.11450241704686992No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8240.11192169827594403No Hit
ATTCAGGGGCAGTGGGTCAGGCACAGATTTTACGCTGACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTA8030.10906932489755225No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8020.1089334975938193No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT8000.10866184298635342No Hit
GAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAA7940.10784687916395579No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7930.10771105186022285No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7920.1075752245564899No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7870.10689608803782519No Hit
ATCTCTAGTCGTGTCCCTTGGCCGAAGGTGATCAGAGTTTGTAGAGATTGCATGCAATAATAAAGGCCAACATC7840.10648860612662636No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT7780.10567364230422871No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7720.10485867848183106No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7720.10485867848183106No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG7660.1040437146594334No Hit
CAGTAATATGTGGCCGTGTCCACAGGGTCCACGTCGGCCAGTCTAAGCAGAACCTGGTTTTTGGAAGTGTCCT7650.10390788735570046No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7600.10322875083703575No Hit
GGCACAGATTTTACGCTGACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTA7520.10214213240717221No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7480.10159882319224046No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT7460.10132716858477459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAG401.4760426E-598.2140570
AAATCGA351.3860482E-462.52190469
GTGGTAT1550.052.5851481
AATGGTA1905.456968E-1251.69160570
TATATAC352.4976778E-548.502883
TGGTATC1700.047.9322622
TGTTAGA7750.045.6220170
ATATACC306.82563E-445.272434
CGTATGC1450.044.94674341
CGTCGGA1104.0693E-544.6427570
TCGTATG1550.042.0123640
GTATCAA15800.039.54981
CCCTAGT350.001457341238.80494315
CTTATAG350.001457827738.8023072
AATCTCG1600.038.44143736
ATCTCCG1900.037.52846510
TATACAC1900.037.5259133
ATCTCGT1550.037.50258637
CGAGACA1950.036.5960223
ATGCCGT1800.036.30674744
Produced by FastQC (version 0.11.5)