FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736229
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29600.40204881904950773No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28450.3864286791202194No Hit
CTACAAACTCTGATCACCTTCGGCCAAGGGACACGACTAGAGATTAAGCG23020.312674453193232No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22060.2996350320348696No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21830.29651100404901193No Hit
CCCCTGGAGAGCCGGCCTCCATATCTTGCAGGTCAAGTCAGAGCCTCCTGCACAATAATGGATACTACTCTTTG21750.2954243856191484No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21240.28849719312876837No Hit
GAGTTTGTAGAGATTGCATGCAATAATAAAGGCCAACATCCTCAGCCTCCACTCTGGTGATTGTCAGCGTAAAAT20840.2830641009794507No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19340.26269000541950943No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTG19100.2594301501299188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18410.2500580661723458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17740.24095763682223872No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17720.24068598221477283No Hit
TTATTATTGCATGCAATCTCTACAAACTCTGATCACCTTCGGCCAAGGGACACGACTAGAGATTAAGCGAACTGT17720.24068598221477283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAACATGAAGACAGATGGTGCAGCCACAGTTC17500.23769778153264814No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17380.23606785388785284No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16790.22805404296760926No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16160.2194969228324339No Hit
GTGCTAATGGAGCGGCTGTGGCCTGGATCCGTCAGCCCCCCGGAAAGGCCCTGGAATGGCTCGCACTCATTTCTT16120.21895361361750215No Hit
GTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15920.2162370675428433No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15070.20469174672554327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14240.1934180805157091No Hit
ATAATGGATACTACTCTTTGGATTGGTATGTGAAGAAGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGTT13940.18934326140372085No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13900.1887999521887891No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCA13760.18689836993652792No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCT13510.18350268734320435No Hit
GTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13100.17793376789015375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT12900.17521722181549493No Hit
GTGCAGCCACAGTTCGCTTAATCTCTAGTCGTGTCCCTTGGCCGAAGGTG12720.17277233034830194No Hit
GCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCTGACTCAGTCTCCACTTTCCCTGGCCGTCACCC12570.17073492079230781No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12570.17073492079230781No Hit
CTCCAGGGGTGACGGCCAGGGAAAGTGGAGACTGAGTCAGCACTATTTCC12460.16924082045124547No Hit
CATGAAGACAGATGGTGCAGCCACAGTTCGCTTAATCTCTAGTCGTGTCC12150.16503017403552428No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGGGAAATAGTGCTGACTCAGTCTCCACTTTCC11730.15932542727874072No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11530.1566088812040819No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11500.15620139929288307No Hit
CAATAATAAAGGCCAACATCCTCAGCCTCCACTCTGGTGATTGTCAGCGTAAAATCTGTGCCTGACCCACTGCCC10970.14900255219503714No Hit
GACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTACAAACTCTGATCACCTT10830.14710096994277597No Hit
CGACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAAT10800.1466934880315771No Hit
CCTTCGGCCAAGGGACACGACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCT10710.14547104229798064No Hit
GAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTACAAACTCTGATCACCTTCGGCCAAGG10510.14275449622332181No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10450.14193953240092416No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG10310.14003795014866297No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGGGA10150.13786471328893593No Hit
GAGTAGTATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAAGATATGGAGGCCGGCTCTCCAGGGGTGACG10020.13609895834040767No Hit
GTTGTGGAGACTGCCCTGGCTTCTTCACATACCAATCCAAAGAGTAGTATCCATTATTGTGCAGGAGGCTCT9880.13419737608814647No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9700.13175248462095354No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT9690.1316166573172206No Hit
GTATGTGAAGAAGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGTTCAAATCGGGCCCTCGGGGTCCCT9620.13066586619109No Hit
ATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAAGATATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGG9580.13012255697615824No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG9540.12957924776122648No Hit
GATTTTACGCTGACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTACAAACT9520.12930759315376059No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9480.1287642839388288No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC9420.12794932011643115No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9330.12672687438283467No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA9270.12591191056043705No Hit
GTAATATGTGGCCGTGTCCACAGGGTCCACGTCGGCCAGTCTAAGCAGAACCTGGTTTTTGGAAGTGTCCTTGGT9260.1257760832567041No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9230.1253686013455053No Hit
GGACACGACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATG9180.12468946482684057No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9170.12455363752310762No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8950.1215654368409829No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATTCAGTGGG8890.12075047301858524No Hit
CTCCACAACTCCTAATATATTTGAGTTCAAATCGGGCCCTCGGGGTCCCTGACAAATTCAGGGGCAGTGGGTCAG8870.12047881841111936No Hit
ACTAGAGATTAAGCGAACTGTGGCTGCACCATCTGTCTTCATGTTCCCGCCATCTGATGAGCAGTTGAAATCTG8850.1202071638036535No Hit
GGGGAAATAGTGCTGACTCAGTCTCCACTTTCCCTGGCCGTCACCCCTGG8760.11898471807005702No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8710.1183055815513923No Hit
GGTGATTGTCAGCGTAAAATCTGTGCCTGACCCACTGCCCCTGAATTTGT8710.1183055815513923No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8570.11640399929913112No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA8550.11613234469166522No Hit
GGTCAAGTCAGAGCCTCCTGCACAATAATGGATACTACTCTTTGGATTGGTATGTGAAGAAGCCAGGGCAGTCTC8500.11545320817300052No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8490.11531738086926757No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8390.11395910783193818No Hit
GCGTAAAATCTGTGCCTGACCCACTGCCCCTGAATTTGTCAGGGACCCCG8380.11382328052820523No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8140.11056342523861462No Hit
ATTCAGGGGCAGTGGGTCAGGCACAGATTTTACGCTGACAATCACCAGAG8130.11042759793488167No Hit
GCCCTGGCTTCTTCACATACCAATCCAAAGAGTAGTATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAA8100.11002011602368285No Hit
ATACTACTCTTTGGATTGGTATGTGAAGAAGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGTTCAAATCG8060.10947680680875108No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7900.10730356994902403No Hit
GGCACAGATTTTACGCTGACAATCACCAGAGTGGAGGCTGAGGATGTTGGCCTTTATTATTGCATGCAATCTCTA7770.10553781500049578No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAACAT7710.10472285117809813No Hit
GTGCTGACTCAGTCTCCACTTTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATATCTTGCAGGTCAAGTCA7690.10445119657063223No Hit
CTTCACATACCAATCCAAAGAGTAGTATCCATTATTGTGCAGGAGGCTCTGACTTGACCTGCAAGATATGGAGGC7680.10431536926689929No Hit
GGCCGAAGGTGATCAGAGTTTGTAGAGATTGCATGCAATAATAAAGGCCAACATCCTCAGCCTCCACTCTGGT7630.10363623274823458No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7620.10350040544450163No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA7480.10159882319224046No Hit
ATCTCTAGTCGTGTCCCTTGGCCGAAGGTGATCAGAGTTTGTAGAGATTGCATGCAATAATAAAGGCCAACATC7470.10146299588850753No Hit
AGCCAGGGCAGTCTCCACAACTCCTAATATATTTGAGTTCAAATCGGGCCCTCGGGGTCCCTGACAAATTCAGGG7450.10119134128104164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA700.003123605964.2339670
AATGGTA2350.063.77840870
AGAGCAT7800.055.72433570
ACTGTCA900.00844523349.9597570
TGGTATC2100.048.3678552
GTGGTAT2300.044.1919751
AAGCGGT3801.8189894E-1243.38609770
AGTAGGT3801.8189894E-1243.38609770
CCACGGT4003.6379788E-1241.21679770
GTCTTCA24700.037.62151370
GAAGTTT4551.6370905E-1136.23454370
GTATCAA13950.036.1877861
ACAGGTA952.6739144E-1035.66371
GCTTGTT601.5191348E-533.8805161
GGTACGG400.00284823933.8528984
CGTCACA9350.033.6627270
GAGCAAA20600.032.74061270
AAACACA19850.032.46754570
AGTTTGT17200.032.24146770
TATCAAC16000.031.3181862
Produced by FastQC (version 0.11.5)