FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994049
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40120.4036018345172119No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA36780.3700018811949914No Hit
GAGTTACAGTGATACTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAAGAACTGTGGCTGCACCAT32080.3227205097535433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27170.27332656639662634No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26280.2643732854215436No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25870.26024874025324707No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT25390.2554200044464609No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAACTGTGTC23210.2334894959906403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC22680.2281577668706472No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT22620.22755417489479896No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21560.2168907166548128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21340.21467754607670245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20020.20139852260804048No Hit
CAGTTCACTCTCACCATCAGTAGTCTGCAACCTGAAGATTTTGCAACTTA19770.19888355604200597No Hit
CTATTATAACGTGCACAATAATATGTGCCTCTGTCTACAGGGTTCATGTT18740.18852189378994397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18460.18570513123598534No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18070.18178178339297157No Hit
GATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACACAGTTCA17610.17715424491146814No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17450.17554466630920607No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16890.16991114120128886No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16880.1698105425386475No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC16480.16578659603299234No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16330.16427761609337166No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16200.16296983347903374No Hit
GTCCCAGGTTTGTGTTGATACCAATTTAAATAGGTGCTAATGCTCTGACT15740.1583422949975303No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15690.1578393016843234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGTATCA15660.15753750569639927No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15660.15753750569639927No Hit
GAGTGAACTGTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAACATA15430.15522373645564758No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT15290.15381535517866826No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTT14890.1497914086730131No Hit
ACCTGGGACAGCCCCTAAGAACCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG14550.14637105414320623No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14350.14435908089037863No Hit
CTACTGATGGTGAGAGTGAACTGTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTG14280.143654890251889No Hit
TTATAATAGTGGTGGAAGACTTAGGGTGGCTGCTTTTGATCTCTGGGGCC14020.14103932502321315No Hit
ACTTACTACTGTCAACAGAGTTACAGTGATACTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAAAGAA13790.13872555578246143No Hit
GCCCCTAAGAACCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTG13470.13550639857793728No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13450.13530520125265455No Hit
ACATAGATCAGGTTCTTAGGGGCTGTCCCAGGTTTGTGTTGATACCAATTTAAATAGGTGCTAATGCTCTGACTT13350.13429921462624075No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13280.1335950239877511No Hit
ACACAAACCTGGGACAGCCCCTAAGAACCTGATCTATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT13010.13087886009643387No Hit
GTGCAGCCACAGTTCTTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC12750.12826329486775803No Hit
CTTAGGGGCTGTCCCAGGTTTGTGTTGATACCAATTTAAATAGGTGCTAATGCTCTGACTTGCCCGGCAAGTGAT12590.12665371626549596No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTACTGATGGTGAGAGTGAACTGTGTCCCAGATCCACTGCCA11880.11951121121795806No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11670.11739863930248912No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11670.11739863930248912No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11620.11689564598928223No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11580.1164932513387167No Hit
GTCTGCATCTGTTGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGA11560.11629205401343394No Hit
GACCTGTACCTTCTCTGGATTCTCACTCAGCATGAGTGGAGTGGGTGTGGCCTGGGTCCGTCAGCCCCCAGGAAA11530.1159902580255098No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11260.11327409413419258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11250.1131734954715512No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11230.11297229814626844No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11210.11277110082098568No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11180.11246930483306156No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACACAGTTCACTCTCACCATCAGTAGTCTGCAACCT11050.11116152221872364No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10980.11045733158023398No Hit
GTTGGAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCTATTTAAATTGGTATCAACACAAA10970.11035673291759258No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA10910.10975314094174432No Hit
CAATAATATGTGCCTCTGTCTACAGGGTTCATGTTGGTCATTGTAAGGACCACCTGGTTTTTGGACGTGTCCTTG10710.10774116768891674No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10710.10774116768891674No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10690.10753997036363398No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10650.10713757571306846No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC10570.10633278641193744No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG10560.10623218774929606No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10550.10613158908665467No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGAT10510.10572919443608916No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10480.10542739844816504No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10450.10512560246024089No Hit
GTCTACAGGGTTCATGTTGGTCATTGTAAGGACCACCTGGTTTTTGGACGTGTCCTTGGTGATGGTGAGTCTGTT10230.10291243188213058No Hit
ATGTTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACACAGTTCACTCTCA10100.10160464926779263No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10070.1013028532798685No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10070.1013028532798685No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10060.10120225461722712No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9980.10039746531609609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCATT650.002816154965.944170
GTGTTCA951.6752363E-460.15952370
TAACAAT750.004963894457.1515570
GCATGTT800.006408035353.57957570
AGTACGT200.00789639850.83508313
TTAACTT850.00814381950.42783770
CGGCAAA1452.2720047E-549.26857470
TGGTTTT14700.043.7384370
AATGGTA3506.184564E-1140.82253370
GTGGTAT2200.040.0700261
TAAGTCA1450.001347943939.4148670
GAGCAAA21800.039.3244670
CTCTTCA10800.038.36562370
GAAGAGT1500.001592303738.10103270
AAGCGGT7600.035.71971570
AACGCTT7100.034.21043470
ATCAACG20850.033.6360863
GAGCCAT8550.033.4219670
TCAACGC21000.033.397514
CAACGCA21250.033.00465
Produced by FastQC (version 0.11.5)