FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595829
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCACCAATAAGAACTACTTAGCTTGGTACCAGCACAA23960.4021287986989555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22480.3772894572100385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21940.36822645423435246No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21240.35647811704364846No Hit
ATATTATAGTAATCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20790.34892561456391014No Hit
CTATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTC19180.3219044390252908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16730.2807852588578266No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGA16440.2759180905931064No Hit
GTAGTATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATATAGCGTGTTCTTGGAATTGTCTCTGGA14810.24856124827760984No Hit
ACTTAGGAGGCTGTCCTAGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTGTATAAAACACT14660.24604374745103041No Hit
AACTAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG14590.24486891373196001No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14060.23597374414471264No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13810.2317779094337469No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13790.23144224265686966No Hit
GTCCTAGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTG13770.23110657587999242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT13290.22305057323493818No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC13050.2190225719124111No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12200.20475673389512764No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11500.1930083967044236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11130.1867985613321943No Hit
ATCACATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTATATCT11070.18579156100156255No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC10960.1839453937287376No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10900.18293839339810583No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10850.18209922645591267No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG10340.1735397236455426No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA10280.17253272331491082No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10130.1700152224883314No Hit
GTATACTACTGTACGAAAGATGCGCGGGGGTGTAGTGGTGGGACCTGCTATTGGGGAGATTTTGACTGCTGGGGC9940.16682638810799744No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTA9730.1633018869507862No Hit
ATAGTAATCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA9700.16279838678547032No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9520.159777385793575No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9480.1591060522398205No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT9360.15709205157855693No Hit
GTTTATTACTGTCACCAATATTATAGTAATCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA9140.1533997170329071No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9070.15222488331383668No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8960.15037871604101177No Hit
GTATGTGATAACACCATCAGAACTAATAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT8910.1495395490988186No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.14870038215662545No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8550.14349754711502796No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT8380.14064437951157127No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8370.14047654612313265No Hit
CTCCTGTGCAGCTTCTGGATTCATCTTTAGTACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG8360.14030871273469403No Hit
GCCGTATACTACTGTACGAAAGATGCGCGGGGGTGTAGTGGTGGGACCTGCTATTGGGGAGATTTTGACTGCTGG8060.13527371108153513No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8050.1351058776930965No Hit
TCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC8050.1351058776930965No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7820.13124570975900804No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7760.13023870942837626No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7750.13007087603993764No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG7650.12839254215555135No Hit
CTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTATATCTGCAAATGAA7520.12621070810584917No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA7440.12486804099834012No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7430.1247002076099015No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC7310.12268620694863795No Hit
GATTTCACTCTCACCATCAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTAAT7190.12067220628737439No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA7080.11882603901454947No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC6960.11681203835328592No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG6950.1166442049648473No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6920.1161407047995314No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA6900.11580503802265416No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6870.11530153785733825No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAC6870.11530153785733825No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6790.11395887074982923No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6680.11211270347700432No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG6640.1114413699232498No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6620.11110570314637254No Hit
GTATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATATAGCGTGTTCTTGGAATTGTCTCTGGAGAT6560.11009870281574075No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTAGTTTGTGCTGGTA6540.1097630360388635No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6520.10942736926198625No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC6450.10825253554291583No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGTAGCCTGCAGGCTGAAGAT6420.10774903537759996No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGG6400.10741336860072269No Hit
GGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAT6380.10707770182384543No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA6350.10657420165852954No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6280.10539936793945914No Hit
TCAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATAGTAATCCGCTCACCTTCGGCG6140.10304970050131833No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6140.10304970050131833No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6100.10237836694756382No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6080.10204270017068655No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG6050.10153920000537067No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5980.10036436628630026No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT5980.10036436628630026No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5960.100028699509423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTTT650.002014384871.74003670
TTTAAGT750.003552267362.17469470
CTCAACA850.00583053254.8600370
CTGATCA2256.816481E-848.358170
TTGCTCA19900.046.86535370
TCTTGTT1703.5266094E-545.71669470
GGTATCA3950.044.4501841
AAGCGGT6350.044.0608170
CCCAATA4450.041.91552770
GTGGTAT1050.041.8043371
TGGTATC1050.041.7552152
GAGCCAT8050.040.54871770
GAGCAAA13800.040.54871770
GACAGGT2000.040.518051
GTATCAA10150.038.588621
GTATAAG350.001497699438.588621
ACTTGCA15450.038.230470
ATTCACT754.9036316E-835.973722
GACAGCT1103.6379788E-1133.765041
ATAAGAG400.002903369733.7197043
Produced by FastQC (version 0.11.5)