FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507999
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG19680.387402337406176No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17960.35354400303937605No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17580.3460636733536877No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC17470.3438983147604621No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG17120.3370085374183807No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15780.31063053273726915No Hit
GACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCATTA15410.30334705383278315No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCC15110.2974415303967134No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14940.29409506711627387No Hit
GTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCG13720.2700792718095902No Hit
CCCCAGGACAGACGGCCACAATCACCTGTGGGGGAACCAATTTTAAAACT13440.26456744993592507No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12940.25472491087580884No Hit
ATACTGGGGCCTGGCCTGTCTTCTGCTGGTACCAGCGCACACTTCTAGTTTTAAAATTGGTTCCCCCACAGGTGA12680.24960679056454838No Hit
CCCCAGTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCGAATCTCTGGCTCCAACTC12360.24330756556607394No Hit
ACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCATTAC12150.2391736991608251No Hit
CTATTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGACTTCGACCCCGCTGATGGTCAGGGTGGCCGTG11950.2352366835367786No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11660.2295280108819112No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11500.22637839838267398No Hit
GGATAATAGTAATGATCATTATGTCTTCGGACCTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC11310.22263823353982978No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCATTACTATTATCCCACAC11180.22007917338419955No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11040.217323262447367No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT10690.21043348510528564No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGAC10490.20649646948123915No Hit
GGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACGGCCACAATCAC10450.20570906635642983No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10240.20157519995118103No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT9840.193701168703088No Hit
GCAGAAGACAGGCCAGGCCCCAGTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCGAA9710.19114210854745778No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCAT9670.19035470542264846No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC9520.18740194370461358No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG9180.18070901714373452No Hit
GTCTTCTGCTGGTACCAGCGCACACTTCTAGTTTTAAAATTGGTTCCCCCACAGGTGATTGTGGCCGTCTGTC8960.17637829995728338No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGTCGGGGATGAGGCCGACTAT8770.17263813511443918No Hit
CCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACG8750.17224443355203456No Hit
CTATTACTGTCAGGTGTGGGATAATAGTAATGATCATTATGTCTTCGGACCTGGGACCGAGGTCACCGTCCTAGG8560.16850426870919036No Hit
GCCCTCAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACACGG8530.1679137163655834No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8440.16614205933476248No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGATTCGCTCAGGGATTCCTGAGGGCCGGTCGCCATCATCATAGA8300.16338614839792992No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8270.16279559605432292No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT8100.1594491327738834No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC8050.1584648788678718No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA7770.15295305699420667No Hit
CCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCC7620.1500002952761718No Hit
TCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACACGGCCACC7620.1500002952761718No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTG7130.14035460699725785No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG7010.13799239762282997No Hit
GGCCAGGCCCCAGTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGG7010.13799239762282997No Hit
GCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACGGCCACAA7000.13779554684162765No Hit
GTAATAGTCGGCCTCATCCCCGACTTCGACCCCGCTGATGGTCAGGGTGG6970.13720499449802068No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT6860.1350396359047951No Hit
CCTGTGGGGGAACCAATTTTAAAACTAGAAGTGTGCGCTGGTACCAGCAGAAGACAGGCCAGGCCCCAGTATTGA6860.1350396359047951No Hit
CCTGAGGGCCGGTCGCCATCATCATAGACGATCAATACTGGGGCCTGGCCTGTCTTCTGCTGGTACCAGCGCACA6670.1312994710619509No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA6610.13011836637473695No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGT6470.12736245543790442No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG6460.12716560465670207No Hit
GTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACGGCCAC6460.12716560465670207No Hit
CATCATAGACGATCAATACTGGGGCCTGGCCTGTCTTCTGCTGGTACCAGCGCACACTTCTAGTTTTAAAATTGG6310.12421284293866719No Hit
CAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACACGGCCACC6260.12322858903265559No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA6240.12283488747025093No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6180.12165378278303697No Hit
CAGTAATAGTCGGCCTCATCCCCGACTTCGACCCCGCTGATGGTCAGGGT6180.12165378278303697No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6110.12027582731462069No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC6070.1194884241898114No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6000.11811046872139512No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5830.1147640054409556No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA5760.11338604997253932No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT5710.11240179606652768No Hit
CTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACGG5580.10984273591089747No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA5520.1086616312236835No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGT5400.10629942184925562No Hit
GTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGG5380.10590572028685095No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG5350.10531516794324398No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5340.10511831716204165No Hit
GTGTTGATAGACTCGTCCGCGGTAATGGAGAGTCTGTCCCGAAACTTGTC5330.10492146638083934No Hit
GACTATTACTGTCAGGTGTGGGATAATAGTAATGATCATTATGTCTTCGG5290.10413406325603003No Hit
CTTCTAGTTTTAAAATTGGTTCCCCCACAGGTGATTGTGGCCGTCTGTCCTGGGGCCACTGACAACGAGGGGGG5200.10236240622520909No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT5190.10216555544400678No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5180.10196870466280446No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTC5150.10137815231919749No Hit
GTCCTGGGGCCACTGACAACGAGGGGGGCTGAGTCAGCACATAGGACGTC5130.10098445075679283No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5110.10059074919438818No Hit
AACTAGAAGTGTGCGCTGGTACCAGCAGAAGACAGGCCAGGCCCCAGTAT5080.10000019685078121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGC150.002421439468.5158240
AATGGTA1456.89397E-1058.21319670
TCGTTAA252.2213293E-456.83390458
GTGCGGT200.00810862850.4915611
TTATACA2050.044.334052
TCTCGTA1550.044.04051638
CGTATGC1900.043.38180541
TCGTATG1900.043.2731640
ATGCCGT2000.043.1161144
GTATGCC2000.042.99468242
GGTATCA3200.042.1053121
ACGAGAC2150.040.71438622
TACACAT2400.040.673755
CTCGTTA350.001183844840.47791757
GTGGTAT1250.040.4210971
TATACAC2350.040.1067733
ATCTCGT1700.040.07858337
CCCACGA2100.040.0766119
GATCTCG1700.040.03471436
CTTATAC2300.039.542381
Produced by FastQC (version 0.11.5)