FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507999
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20460.4027566983399573No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18930.3726385288160016No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC17690.3482290319469133No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAG16260.3200793702349808No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG15090.2970478288343087No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG15050.29626042570949945No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT14760.290551753054632No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14630.28799269289900176No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCC14330.282087169462932No Hit
GACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCATTA14100.27755960149527853No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13170.2592524788434623No Hit
CCCCAGGACAGACGGCCACAATCACCTGTGGGGGAACCAATTTTAAAACT12540.2468508796277158No Hit
CCCCAGTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTG11790.2320870710375414No Hit
GTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCG11720.23070911556912513No Hit
ACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCATTACTATTATCCCACACCTGACAGTAATA11540.22716580150748325No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11030.2171264116661647No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGGTCCGAAGACATAATGATCATTACTATTATCCCACAC10840.2133862468233205No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC10670.210039783542881No Hit
CTATTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGACTTCGAC10590.20846497729326238No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC10590.20846497729326238No Hit
GGATAATAGTAATGATCATTATGTCTTCGGACCTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC10330.20334685698200194No Hit
GGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACGGCCACAATCAC10270.202165752294788No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGATTCGCTCAGGGATTCCTGAGGGCCGGTCGCCATCATCATA10140.19960669213915774No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA10120.1992129905767531No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGT10070.1982287366707415No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACT10050.19783503510833683No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT9770.19232321323467172No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9690.19074840698505313No Hit
CCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACA9320.18346492808056708No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG9030.17775625542569964No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG8850.1742129413640578No Hit
ATACTGGGGCCTGGCCTGTCTTCTGCTGGTACCAGCGCACACTTCTAGTTTTAAAATTGGTTCCCCCACAGGT8680.17086647808361827No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8370.1647641038663462No Hit
GCAGAAGACAGGCCAGGCCCCAGTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCGAAT8340.16417355152273924No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG8110.15964598355508575No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8090.1592522819926811No Hit
CTATTACTGTCAGGTGTGGGATAATAGTAATGATCATTATGTCTTCGGAC8000.15748062496186016No Hit
CCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACG7990.15728377418065784No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCG7890.15531526636863457No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG7610.1498034444949695No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT7600.14960659371376714No Hit
GTCTTCTGCTGGTACCAGCGCACACTTCTAGTTTTAAAATTGGTTCCCCCACAGGTGATTGTGGCCGTCTGTC7520.14803178746414855No Hit
GCCCTCAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACACGG7490.14744123512054158No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT7390.14547272730851832No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTA7350.14468532418370902No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT7230.14232311480928114No Hit
CCTGTGGGGGAACCAATTTTAAAACTAGAAGTGTGCGCTGGTACCAGCAGAAGACAGGCCAGGCCCCAGTATT7230.14232311480928114No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC7110.13996090543485323No Hit
GCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCA6900.13582703902960439No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTC6810.13405538199878345No Hit
CGGCCACCCTGACCATCAGCGGGGTCGAAGTCGGGGATGAGGCCGACTAT6770.13326797887397415No Hit
GGCCAGGCCCCAGTATTGATCGTCTATGATGATGGCGACCGGCCCTCAGG6540.12874041090632068No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT6420.12637820153189278No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC6410.12618135075069045No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA6360.12519709684467883No Hit
TCTATGATGATGGCGACCGGCCCTCAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACACGGCCACC6350.1250002460634765No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6320.12440969371986953No Hit
CAGTAATAGTCGGCCTCATCCCCGACTTCGACCCCGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCA5890.11594511012816955No Hit
CAGGAATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCGGGGTCGAAGTCG5780.11377975153494396No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACT5750.11318919919133699No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG5690.11200809450412305No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5630.11082698981690908No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC5450.10728367575526723No Hit
GTAATAGTCGGCCTCATCCCCGACTTCGACCCCGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGA5390.10610257106805328No Hit
GGACAGACGGCCACAATCACCTGTGGGGGAACCAATTTTAAAACTAGAAGTGTGCGCTGGTACCAGCAGAAGACA5380.10590572028685095No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTG5300.10433091403723237No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAG5290.10413406325603003No Hit
CTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACG5280.1039372124748277No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGA5250.10334666013122074No Hit
GTGACGTCCTATGTGCTGACTCAGCCCCCCTCGTTGTCAGTGGCCCCAGGACAGACGGCCACAATCACCTGTGGG5240.1031498093500184No Hit
GTGTTGATAGACTCGTCCGCGGTAATGGAGAGTCTGTCCCGAAACTTGTCTGCGGAGTTTGTTGTTCCAAAGACA5230.10295295856881609No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG5190.10216555544400678No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC5190.10216555544400678No Hit
CTTCTAGTTTTAAAATTGGTTCCCCCACAGGTGATTGTGGCCGTCTGTCCTGGGGCCACTGACAACGAGGGGG5130.10098445075679283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA200.007329776143.3488870
AATGGTA1550.0101.7314770
GCGAGAG150.002519644667.8323235
GTAACAC150.00262457467.137913
CTGTTTA650.002776343466.16102670
AAGCGGT3450.062.32560370
GTAGTTT700.003723950461.43523870
TATTCTA750.004893831457.33955870
GAGAAAT800.006317660753.7558370
CTTGACA200.007445720551.59258743
GTCGTAA850.00802905850.59372370
CTAGTAC200.00819371550.3583878
TAACACT200.00819371550.3583874
AGAGCGT200.00819371550.3583878
TAGGCAA2005.0960807E-850.17210870
ATACGTA307.20781E-444.77182411
TCTTGTT2659.502401E-943.27513570
ACTGCTA1359.313979E-442.47374370
AAAACAT1359.313979E-442.47374370
GCCGTGT11700.040.4317470
Produced by FastQC (version 0.11.5)