FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968238
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAAATCTCAGGCTGTTCAGTTCCATGTAGG39710.41012643585564706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT25520.2635715598850696No Hit
GTCTATTACTGTGCGATAGGTTGGGGTGGCAACTGGGCCGGGGACTATTGGGGCCAGGGAACTCTGGTCACCG24870.25685833441777745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23780.24560077171108755No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19020.19643930521214825No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGACACTTTTAGCAC18600.1921015287563595No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18210.1880735934759842No Hit
GTATGGTAGCTCACCTCAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA17640.18218661114312804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17250.17815867586275275No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16530.17072248765282916No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT16350.16886344060034827No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGTA15700.16215021513305614No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15480.15987804651335724No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATAGATGAGGAGCC15300.15801899946087636No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14840.15326810143786962No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14740.15223529751982467No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14600.15078937203456175No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14420.14893032498208086No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAG14240.14707127792959995No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14000.14459254852629208No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13840.14294006225742018No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13820.1427335014738112No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13560.14004821128689435No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13520.13963508971967636No Hit
GTTTATTACTGTCAGCAGTATGGTAGCTCACCTCAGCTCACTTTCGGCGG13400.13839572501802244No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13270.137053079924564No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13260.1369497995327595No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13240.13674323874915054No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGGTGGTACCAGGCTAAGTAGGTGCTACTAAGACTCTGA13050.13478091130486514No Hit
CTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC12970.13395466817042917No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG12580.1299267328900539No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12570.1298234524982494No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12490.12899720936381345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12260.12662176035231007No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12230.1263119191768966No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGCACCTACTTAGCC12230.1263119191768966No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11960.12352334859817524No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCTGAGGTGAGCTACCATAC11890.12280038585554377No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGGTCATCCCTATCTTTGGT11870.12259382507193478No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11790.12176758193749883No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11620.12001181527682243No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11580.11959869370960446No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11400.11773964665712357No Hit
CTTTTAGCACCTCTGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGGTCATCC11380.11753308587351458No Hit
GAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGAT11140.1150543564702067No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11130.1149510760784022No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11070.11433139372757524No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGCA10980.1134018702013348No Hit
CTTCTGCAGGGCCAGTCAGAGTCTTAGTAGCACCTACTTAGCCTGGTACC10900.11257562706689885No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATAGA10850.11205922510787639No Hit
GTCTTAGTAGCACCTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA10780.11133626236524491No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10690.11040673883900445No Hit
GTTCAGTTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCCTGA10670.11020017805539546No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10550.10896081335374154No Hit
GCCTACATGGAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGATAGGTTGGGGTGGCAA10540.10885753296193705No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10540.10885753296193705No Hit
GTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10470.10813457021930559No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10330.10668864473404266No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG10140.10472631728975726No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10040.10369351337171231No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10030.10359023297990783No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9930.10255742906186288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA12950.063.20500670
ATAGCAA600.00696385752.46825870
ATAGGAC200.00843070749.9998323
CGTATGC1400.041.63718441
TCGTATG1400.041.55770540
GGTATCA6750.041.500631
GCAATCT1550.041.38151634
ATCTCGT1500.040.76832637
CTTATAC1600.039.6016041
AACTGTA1359.218973E-538.86537670
AATCTCG1500.038.41445536
ATGCCGT1750.037.46138444
GGATTAG400.00198595636.43604361
CCCACGA1750.036.19970319
TTATACA1750.036.1903532
TATACAC1750.036.1903533
TAGGCAA3800.035.89933470
AGGCAAT1800.035.511532
GTATGCC1850.035.29493742
GCGTAGA15600.034.97883670
Produced by FastQC (version 0.11.5)