FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968238
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAAATCTCAGGCTGTTCAGTTCCATGTAGG33430.34526634980242465No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25910.26759949516544485No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA24050.2483893422898089No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23760.24539421092747857No Hit
GTCTATTACTGTGCGATAGGTTGGGGTGGCAACTGGGCCGGGGACTATTGGGGCCAGGGAACTCTGGTCACCGT23120.23878426585199095No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18040.1863178268153078No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17660.182393171926737No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGACACTTTTAGCAC17230.17795211507914377No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16880.17433730136598646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT16170.16700439354786736No Hit
GTATGGTAGCTCACCTCAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16170.16700439354786736No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15730.16246005630846963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC15210.1570894759346359No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15030.155230428882155No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14360.1483106426312539No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14120.14583191322794603No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG14110.14572863283614151No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA13970.14428270735087861No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13760.14211381912298424No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATAGATGAGGAGCCTG13750.14201053873117972No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13490.13932524854426287No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13430.1387055661934359No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC13240.13674323874915054No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA13090.1351940328720831No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13080.1350907524802786No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGGTGGTACCAGGCTAAGTAGGTGCTACTAAGACTCT12770.13188906033433928No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTA12750.1316824995507303No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGCACCTACTTAGCCTG12570.1298234524982494No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12460.12868736818839996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12320.12724144270313706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12290.12693160152772356No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12210.1261053583932876No Hit
GTTTATTACTGTCAGCAGTATGGTAGCTCACCTCAGCTCACTTTCGGCGG11890.12280038585554377No Hit
CTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11480.11856588979155952No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11470.11846260939975502No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11440.11815276822434154No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGGTCATCCCTATCTTTGGT11410.11784292704892804No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGCA11380.11753308587351458No Hit
GTTCAGTTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCCTGAGTCTGCCCTGGAACTTCTGTGCGTA11220.11588059960464267No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11130.1149510760784022No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCTGAGGTGAGCTACCATACT10900.11257562706689885No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10660.11009689766359099No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC10370.10710176630126064No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA10310.10648208395043367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10260.10596568199141121No Hit
CTTTTAGCACCTCTGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGGTCATCC10240.10575912120780222No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATA10120.10451975650614827No Hit
GTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10060.10390007415532132No Hit
GCCTACATGGAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGATAGGTTGGGGTGGCAA10050.10379679376351682No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10040.10369351337171231No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT10000.10328039180449436No Hit
GAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGATAGGTTGGGGTGGCAACTGGGCCGGG9970.10297055062908086No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9820.10142134475201346No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT9730.100491821225773No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9720.10038854083396852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9710.10028526044216401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAT454.1182255E-4107.0044870
CGCGGTA13100.084.5417170
ATTAGTT600.001291887880.25336570
GGACCAT950.00797917150.68633770
TCTAGTC200.00843052850.0000923
TCTTATA354.5346795E-449.18831368
GAGGGTT1355.34549E-447.55755270
ACTCAGT11100.044.82620270
GAGCAAA15550.043.35229570
TGCTGTA8200.043.0627870
GGTATCA6950.039.3062441
GAGCCAT12400.038.83227570
AAGCGGT8800.038.3027470
TAGGCAA3852.2646418E-937.5210570
TGGAGTT23850.037.0141370
CCCCGAA900.003440989635.6681670
GTATCAA16000.035.6048051
TCCATAT1900.002868999733.7908970
CTGGATA1900.002868999733.7908970
CTCGTAT1458.326306E-633.20828670
Produced by FastQC (version 0.11.5)