FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894286
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39880.4459423495391855No Hit
GTAATAGACGGCCGAGTCGTCGACTCGAAGGCTGAGCATTTGCAGAAACA36540.408594118660026No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT35800.400319360920332No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26330.29442482606235587No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25630.2865973525248075No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24960.27910534213886834No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24000.2683705212873734No Hit
GTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACTTCTTATCACACGAA23410.26177307930572546No Hit
GTCTATTACTGTGCGAAAGGCCTGATGAACGTCCCCTGTCTGGACTACTGGGGCCAGGGGGCCCACGTCACCG22890.25595838467783233No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21090.23583059558127936No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC20980.23460056402537893No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20870.23337053246947842No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20830.2329232482673328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20530.22956861675124066No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG20270.2266612694372941No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18880.21111814341273374No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18590.20787533294717797No Hit
CACCATCACCAGCGTGCAGGCTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTT18420.2059743750880591No Hit
GGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18180.20329066987518535No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15830.17701272299913004No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15730.17589451249376598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15720.17578269144322956No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15530.17365809148303787No Hit
CCTTTATTGGGCCTCTATTCGGGAAGATGGGGTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCA15120.16907342841104522No Hit
GATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTT14990.16761975475407198No Hit
GCCACAGTTCGTGTGATAAGAAGTTTGGTCCCAGGGCCGAAAGTGGGAGGAAAAGTAAAGTATTGTTGACAGTAG14930.16694882845085351No Hit
GTCCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGGTCCAA14870.1662779021476351No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCAACGGGGACTTCGTGATGACCCAG14580.16303509168207933No Hit
GTGCAGCCACAGTTCGTGTGATAAGAAGTTTGGTCCCAGGGCCGAAAGTG14560.16281144958100652No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14240.15923317596384157No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13840.1547603339423853No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13820.15453669184131252No Hit
GAACGTATACTACGCGGACTCGGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGGACACAGTGTT13750.1537539444875577No Hit
GTATACGTTCCAACCATCACTACTAATACCTGCGACCCATTCCGGCCCCTTCCCTGGAGCCTGGCGGACCCAAT13680.15297119713380283No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13560.15162934452736596No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13290.14861017616288302No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13120.14670921830376413No Hit
AACCAGGACAGCCTCCTCATCTGCTCCTTTATTGGGCCTCTATTCGGGAAGATGGGGTCCCTGACCGATTCAG13100.14648557620269131No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12870.14391369204035398No Hit
CTATTCGGGAAGATGGGGTCCCTGACCGATTCAGTGGCAGCGGATCTGGG12620.14111816577694383No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12550.140335418423189No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12340.13798717636192448No Hit
GCCTGGGACGTCCCTGAGACTCTCCTGTGAAGCCTCAGGATTCACCTTTAGAAACTATGTCATGAATTGGGTCCG12290.13742807110924246No Hit
GTAGAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGACAGGGACTCTGGAGA12190.1363098606038784No Hit
GTTTTCTACAGGTCCAATAATAAGAATTACTTAGCTTGGTATCAACAGAAACCAGGACAGCCTCCTCATCTGCTC12130.13563893430065996No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12020.1344089027447595No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11920.13329069223939544No Hit
GTATTAGTAGTGATGGTTGGAACGTATACTACGCGGACTCGGTGAAGGGCCGGTTCACCATCTCCAGAGACAAT11840.1323961238351042No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11800.1319488396329586No Hit
GTAGTAAACTGCCACATCTGCAGCCTGCACGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA11750.13138973438027657No Hit
ATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGATGTGGCAGTTTA11610.12982423967276688No Hit
GATGAGGAGGCTGTCCTGGTTTCTGTTGATACCAAGCTAAGTAATTCTTATTATTGGACCTGTAGAAAACAC11300.12635778710613832No Hit
GGCTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACT11220.12546321870184707No Hit
CCCTGAGACTCTCCTGTGAAGCCTCAGGATTCACCTTTAGAAACTATGTC11210.12535139765131068No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11180.12501593449970144No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11140.12456865029755582No Hit
GATCTGGGACAGATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGAT11130.12445682924701941No Hit
CTACTAATACCTGCGACCCATTCCGGCCCCTTCCCTGGAGCCTGGCGGAC11090.12400954504487378No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGA10860.12143766088253645No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATAAGAAGTTTGGTCC10710.11976034512449038No Hit
GTCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGA10700.11964852407395397No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG10670.11931306092234474No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10670.11931306092234474No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10590.1184184925180535No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10580.1183066714675171No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10510.11752392411376227No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10490.11730028201268947No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10470.11707663991161665No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10420.11651753465893461No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG10350.11573478730517978No Hit
GAATTACTTAGCTTGGTATCAACAGAAACCAGGACAGCCTCCTCATCTGC10210.1141692925976701No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10160.11361018734498807No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG10160.11361018734498807No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT10100.11293926104176963No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9920.11092648213211433No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9850.1101437347783595No Hit
CTCCTCATCTGCTCCTTTATTGGGCCTCTATTCGGGAAGATGGGGTCCCTGACCGATTCAGTGGCAGCGGATC9830.10992009267728668No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9770.10924916637406826No Hit
CTTTCGGCCCTGGGACCAAACTTCTTATCACACGAACTGTGGCTGCACCA9710.10857824007084983No Hit
CTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACT9690.10835459796977702No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9600.10734820851494935No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9580.10712456641387655No Hit
CAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACTTCTTATCACACGAACTGTGGCTGCACCA9570.10701274536334014No Hit
GTCCTGGTTTCTGTTGATACCAAGCTAAGTAATTCTTATTATTGGACCTG9510.10634181906012172No Hit
CTCCTGTGAAGCCTCAGGATTCACCTTTAGAAACTATGTCATGAATTGGG9510.10634181906012172No Hit
GTCTCCAGAGTCCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTA9410.10522360855475764No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9390.10499996645368483No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9330.1043290401504664No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9260.10354629279671157No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9250.10343447174617516No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9240.10332265069563876No Hit
GATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAACG9220.10309900859456596No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9040.10108622968491064No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9030.10097440863437424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACA400.001872496773.0976670
TTCATAG500.004534235758.47812770
TCGTATG1350.053.3682140
TAACGCA200.006979457652.44667457
AATGGTA3300.050.20849270
CGTATGC1500.048.0612841
CGAGACA1500.047.5421823
ATCTCCG1500.047.4996510
GCGTAGA23250.043.59659270
TCTCCGA1650.043.181511
AATCTCG1200.042.72092436
GTGGTAT1750.042.6718331
TATACAC1750.042.655133
ATGCCGT1700.042.5128444
ACGAGAC1700.041.9302122
TACACAT1700.041.91385
TTATACA1900.041.073422
CACGAGA1750.040.73220421
ATCTCGT1300.039.4702737
GTCGTAT350.00139050139.17828839
Produced by FastQC (version 0.11.5)