FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894286
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA35130.39282735053439277No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34790.389025434816155No Hit
GTAATAGACGGCCGAGTCGTCGACTCGAAGGCTGAGCATTTGCAGAAACACTGTGTCCCTGGAATTGTCTCTGGA30260.3383704989231633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27680.3095206678847706No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT25950.2901756261419725No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24580.2748561422184849No Hit
GTCTATTACTGTGCGAAAGGCCTGATGAACGTCCCCTGTCTGGACTACTGGGGCCAGGGGGCCCACGTCACCGT21840.24421717437150978No Hit
GTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACTTCTTATCACACGAA21410.23940886919844437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20730.23180503776196876No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20570.23001590095338628No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20300.22699673258890332No Hit
GTAGTAAACTGCCACATCTGCAGCCTGCACGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCA20210.22599034313407568No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT19050.21301910127185264No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18610.20809897504825078No Hit
CACCATCACCAGCGTGCAGGCTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTTT18440.20619801718913192No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18040.20172517516767569No Hit
GGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17930.20049514361177523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17750.19848236470211988No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17430.1949040910849549No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17350.19400952268066368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16950.18953668065920745No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA16770.18752390174955216No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15930.17813093350449408No Hit
GTGCAGCCACAGTTCGTGTGATAAGAAGTTTGGTCCCAGGGCCGAAAGTG14830.16583061794548948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14740.16482422849066183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14530.1624759864293973No Hit
GCCACAGTTCGTGTGATAAGAAGTTTGGTCCCAGGGCCGAAAGTGGGAGG14160.1583386075595503No Hit
GAACGTATACTACGCGGACTCGGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGGACACAGTGTTT13700.15319483923487565No Hit
GATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTT13660.15274755503273002No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13480.15073477612307473No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC13220.1478274288091282No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT13140.14693286040483694No Hit
GCCTGGGACGTCCCTGAGACTCTCCTGTGAAGCCTCAGGATTCACCTTTAGAAACTATGTCATGAATTGGGTCC12770.14279548153498994No Hit
GTATACGTTCCAACCATCACTACTAATACCTGCGACCCATTCCGGCCCCTTCCCTGGAGCCTGGCGGACCCAATT12760.14268366048445352No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12290.13742807110924246No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12240.13686896585656044No Hit
GTCCCTGTCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA12100.13530347114905075No Hit
GGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCATCTTCCCGAATAGA11980.1339616185426139No Hit
GGCTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTTCCTC11970.13384979749207748No Hit
CCTTTATTGGGCCTCTATTCGGGAAGATGGGGTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCA11950.13362615539100467No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11800.1319488396329586No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11720.13105427122866734No Hit
CTATTCGGGAAGATGGGGTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCACCA11490.12848238706633003No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG11320.1265814292072111No Hit
GTATTAGTAGTGATGGTTGGAACGTATACTACGCGGACTCGGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT11190.12512775555023783No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10950.12244405033736412No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10940.12233222928682769No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10710.11976034512449038No Hit
AACCAGGACAGCCTCCTCATCTGCTCCTTTATTGGGCCTCTATTCGGGAAGATGGGGTCCCTGACCGATTCAGTG10600.11853031356858991No Hit
ATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGATGTGGCAGTTTA10590.1184184925180535No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10580.1183066714675171No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10400.1162938925578618No Hit
GAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACCCCATCTTCCCGAATAGAGGCCCAATAAA10350.11573478730517978No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10230.11439293469874291No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10160.11361018734498807No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10140.11338654524391525No Hit
GTATTGTTGACAGTAGTAAACTGCCACATCTGCAGCCTGCACGCTGGTGATGGTGAGAGTGAAATCTGTCCCA10110.11305108209230603No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC9910.11081466108157792No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9840.11003191372782309No Hit
CTGCAGATGTGGCAGTTTACTACTGTCAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACTT9830.10992009267728668No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9810.10969645057621387No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC9760.10913734532353185No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9760.10913734532353185No Hit
CTACTAATACCTGCGACCCATTCCGGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGACATAGTTTCTAAAG9690.10835459796977702No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA9690.10835459796977702No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9660.10801913481816779No Hit
GATCTGGGACAGATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGAT9650.10790731376763138No Hit
GATGAGGAGGCTGTCCTGGTTTCTGTTGATACCAAGCTAAGTAATTCTTATTATTGGACCTGTAGAAAACACT9600.10734820851494935No Hit
GTAGAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGACAGGGACTCTGGA9520.10645364011065811No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG9510.10634181906012172No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9440.10555907170636687No Hit
GTTTTCTACAGGTCCAATAATAAGAATTACTTAGCTTGGTATCAACAGAAACCAGGACAGCCTCCTCATCTGCTC9430.10544725065583045No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9410.10522360855475764No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9350.10455268225153921No Hit
GTCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTCTACAGGTCCAATAATAA9330.1043290401504664No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9310.10410539804939359No Hit
TCTGCAGCCTGCACGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCGCTGCCACTGAATCGGTCAGGGACC9260.10354629279671157No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9240.10332265069563876No Hit
GTCCTGGTTTCTGTTGATACCAAGCTAAGTAATTCTTATTATTGGACCTG9230.10321082964510235No Hit
CAACAATACTTTACTTTTCCTCCCACTTTCGGCCCTGGGACCAAACTTCT9140.10220444019027469No Hit
CCCTGAGACTCTCCTGTGAAGCCTCAGGATTCACCTTTAGAAACTATGTCATGAATTGGGTCCGCCAGGCTCCAG9090.10164533493759267No Hit
GTCCCTGACCGATTCAGTGGCAGCGGATCTGGGACAGATTTCACTCTCACCATCACCAGCGTGCAGGCTGCAGAT9040.10108622968491064No Hit
CCTTGGGGCTGGTCGGGGATGCCGAGGAGACGGTGACGTGGGCCCCCTGG8950.100079840230083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)