FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872441
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTGCATTTGCAGATACA58980.6760342533191357No Hit
GTGTATTACTGTGCGAGAGATCGAGATTATTATGGTTCGGGAGACTACTA38890.4457608021631262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23220.2661498026800666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT22610.25915792586547404No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20620.2363483605195079No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19790.22683482321440648No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTGCATTTGCAGATACAGTGTGTTCTTGGAAGTGTCTC18900.21663356032098446No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18530.2123925858596742No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC17810.20413987879982715No Hit
GTATTACTGTGCGAGAGATCGAGATTATTATGGTTCGGGAGACTACTACTACTACGACGGTATGGACGTCTGGGG17660.20242056482902568No Hit
GGGCTGGAGTGGGTGGCAGTTATATGGCATGATGGAAGTAATGAATACTA17630.20207670203486539No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG16910.19382399497501837No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGTGCATGCCATAGTTAA16790.1924485437983772No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16600.19027074610202868No Hit
ACTCAGGACACACCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16420.18820756933706692No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16240.18614439257210516No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15700.17995486227721988No Hit
GCCTGAGACCCGAGGACACGGCTGTGTATTACTGTGCGAGAGATCGAGAT15700.17995486227721988No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15690.17984024134583312No Hit
GGACACACCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATG15620.17903789482612578No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15600.17880865296335224No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14440.1655126249224876No Hit
CACCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCA14220.16299096443197877No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14210.16287634350059202No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14090.16150089232395085No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14090.16150089232395085No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13890.15920847369621557No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCCAAGAACACACTGTATCTGCAAATGCA13860.15886461090205528No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13850.1587499899706685No Hit
CTCCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGCATGCACTGGGTCCGCCAGGCCCCAGGCAAGGGG13520.15496749923490527No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13520.15496749923490527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13460.15427977364658468No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13170.15095576663636853No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12550.14384926889038915No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12490.14316154330206857No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC12250.14041064094878622No Hit
CCCTGAGACTCTCCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGC12130.13903518977214505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12120.1389205688407583No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG12030.1378889804582774No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11910.13651352928163624No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGA11900.13639890835024948No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGATGTCCAGTGTCAGGTGCAGGTGGTGGAG11820.13548194089915536No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11750.13467959437944801No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11670.1337626269283539No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11640.1334187641341936No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG11540.13227255482032596No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11490.13169945016339213No Hit
GATCTATGCTGCATCCTATTTACAAGATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA11480.13158482923200537No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG11440.13112634550645832No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA11400.13066786178091125No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGCAA11300.1295216524670436No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAA11190.1282608222217892No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG11030.126426887319601No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11010.12619764545682743No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT10910.12505143614295983No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA10910.12505143614295983No Hit
GCATAGATCAGGAGATTAGGGGCTTTCCCTGGTTGCTGCTGATACCAGCCTAAATCATTTCTAATGCCCTGACT10820.12401984776047893No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG10780.12356136403493187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC10650.12207129192690394No Hit
GTATTCATTACTTCCATCATGCCATATAACTGCCACCCACTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAG10550.12092508261303629No Hit
CCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10420.11943501050500838No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG10410.1193203895736216No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10410.1193203895736216No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10400.11920576864223482No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10320.11828880119114071No Hit
ACTTATTACTGTCTACAAGATTACAATTACCCACTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA10280.11783031746559365No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10280.11783031746559365No Hit
CTTTTAAGAGATGTCCAGTGTCAGGTGCAGGTGGTGGAGTCTGGGGGAGG10200.11691335001449954No Hit
CGTCCATACCGTCGTAGTAGTAGTAGTCTCCCGAACCATAATAATCTCGATCTCTCGCACAGTAATACACAGCCG10180.11668410815172603No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10140.11622562442617895No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10100.11576714070063192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10070.11542327790647162No Hit
AATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCCAAGAACACACTGTATC9980.11439168952399074No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT9880.1132454802101231No Hit
GGGTCCGCCAGGCCCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGCATGATGGAAGTAATGAATACTA9850.1129016174159628No Hit
ACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGTCGTAG9820.11255775462180251No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGAAGCGTCTGGATTCACCCTCATTAA9670.11083844065100104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9540.10934836854297311No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGATGTCCAGTG9520.10911912668019957No Hit
CTACTACTACTACGACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAG9400.10774367550355841No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCT9390.10762905457217165No Hit
GGCATGCACTGGGTCCGCCAGGCCCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGCATGATGGAAGTAA9370.10739981270939812No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG9350.10717057084662458No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTAC9240.10590974060137018No Hit
GTATCTGCAAATGCACAGCCTGAGACCCGAGGACACGGCTGTGTATTACTGTGCGAGAGATCGAGATTATTATGG9240.10590974060137018No Hit
CCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGCATGCACTGGGTCCGCCAGGCCCCAGGCAAGGGGC9130.10464891035611577No Hit
GATCAGGAGATTAGGGGCTTTCCCTGGTTGCTGCTGATACCAGCCTAAAT9080.10407580569918196No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAGTGGGTAAT8950.10258573359115401No Hit
GTCTGCATAGTATTCATTACTTCCATCATGCCATATAACTGCCACCCACT8920.10224187079699372No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGCATGCACTGGGTCCG8910.10212724986560695No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG8830.10121028241451284No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA8770.10052255682619225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG18350.065.8097170
GTGGTAT1200.045.4763451
TGTTAGA5450.045.40563270
GTATCAA12500.043.657291
TATCAAC14050.038.34452
GAGCAAA11950.037.2744170
TGAGCGG1608.110781E-637.119170
CAGTTCG10150.037.0581570
CTCTGGA17500.036.9074169
ATCAACG14600.036.9000133
TCAACGC14800.036.4034544
CAACGCA15000.035.9180765
AACGCAG15050.035.34566
GCGTAGA14650.035.1343570
TCGTATG1102.910383E-1134.30792640
TCTCGCG10350.033.47294270
ACGCAGA16000.033.239337
CGTATGC1154.9112714E-1132.83711241
CGTACTA1057.166818E-1032.5222729
TCTTATA959.857104E-932.302882
Produced by FastQC (version 0.11.5)