FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872441
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTGCATTTGCAGATACAGTGTGTTCTTGGAAGTGTCTCTGGA52990.6073763154184638No Hit
GTGTATTACTGTGCGAGAGATCGAGATTATTATGGTTCGGGAGACTACTACTACTACGACGGTATGGACGTCTGG37620.43120394387700717No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20780.23818229542169617No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA20740.23772381169614906No Hit
CAGTAATACACAGCCGTGTCCTCGGGTCTCAGGCTGTGCATTTGCAGATACAGTGTGTTCTTGGAAGTGTCTCTG17500.20058662992683746No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17480.20035738806406392No Hit
ACTCAGGACACACCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA17460.20012814620129044No Hit
GGGCTGGAGTGGGTGGCAGTTATATGGCATGATGGAAGTAATGAATACTA17270.19795034850494186No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC17230.19749186477939482No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16850.19313626938669778No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16710.19153157634728307No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16570.1899268833078684No Hit
GCCTGAGACCCGAGGACACGGCTGTGTATTACTGTGCGAGAGATCGAGAT16220.18591515070933165No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16200.1856859088465581No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGTGCATGCCATAGTTAATGAGGGTGAATCCAGACGCTTCACA16180.18545666698378457No Hit
GTATTACTGTGCGAGAGATCGAGATTATTATGGTTCGGGAGACTACTACTACTACGACGGTATGGACGTCTGGG15860.18178879717940813No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14800.1696389784524111No Hit
GGACACACCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT14740.16895125286409052No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14570.1670026970305155No Hit
CTCCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGCATGCACTGGGTCCGCCAGGCCCCAGGCAAGGG14170.16241785977504497No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14140.16207399698088468No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14060.16115702952979055No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT13980.16024006207869643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13950.15989619928453616No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13600.1558844666859994No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13480.15450901550935822No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13220.15152887129330236No Hit
CACCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT13080.14992417825388765No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12700.1455685828611906No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCCAAGAACACACTGTATCTGCAAATGCA12290.14086912467433327No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG12050.13811822232105095No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA11990.13743049673273036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC11750.13467959437944801No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA11740.13456497344806123No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCC11670.1337626269283539No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11550.13238717575171272No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCC11430.13101172457507154No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11330.1298655152612039No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11190.1282608222217892No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGA11100.12722923383930831No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG11000.1260830245254407No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGATGTCCAGTGTCAGGTGCAGGTGGTGGAGT10900.12493681521157304No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA10820.12401984776047893No Hit
CCCTGAGACTCTCCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGCATGCACTGGGTCCGCCAGGCCCCAG10800.12379060589770541No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC10780.12356136403493187No Hit
GAGTGAAATCTGTGCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCATCTTGTAAATAGGATGCAGCATA10600.12149818726997011No Hit
GATCTATGCTGCATCCTATTTACAAGATGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA10590.12138356633858335No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10510.12046659888748926No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT10500.12035197795610247No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10380.1189765267794613No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10370.11886190584807453No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGCAA10370.11886190584807453No Hit
GGGTCCGCCAGGCCCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGCATGATGGAAGTAATGAATACTAT10270.11771569653420691No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT10210.11702797094588632No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGATGTCCAGTGT10180.11668410815172603No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA10040.11507941511231133No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10030.11496479418092456No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC9990.11450631045537749No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGAAGCGTCTGGATTCACCCTCATTAA9940.11393320579844368No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAG9920.11370396393567016No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC9910.11358934300428339No Hit
GTATTCATTACTTCCATCATGCCATATAACTGCCACCCACTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGT9900.1134747220728966No Hit
GCATAGATCAGGAGATTAGGGGCTTTCCCTGGTTGCTGCTGATACCAGCCTAAATCATTTCTAATGCCCTGACTT9900.1134747220728966No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTACAATTAC9890.11336010114150986No Hit
AATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCCAAGAACACACTGTATCT9850.1129016174159628No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC9770.11198464996486869No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9730.11152616623932163No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9590.10992147319990693No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT9570.1096922313371334No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC9470.10854602202326576No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT9290.106482845258304No Hit
ACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTG9240.10590974060137018No Hit
CTTTTAAGAGATGTCCAGTGTCAGGTGCAGGTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCT9230.10579511966998341No Hit
GTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACA9160.10499277315027607No Hit
ACTTATTACTGTCTACAAGATTACAATTACCCACTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA9120.104534289424729No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTG9120.104534289424729No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9100.10430504756195548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9060.10384656383640842No Hit
GTATCTGCAAATGCACAGCCTGAGACCCGAGGACACGGCTGTGTATTACT9040.1036173219736349No Hit
CCTGTGAAGCGTCTGGATTCACCCTCATTAACTATGGCATGCACTGGGTCCGCCAGGCCCCAGGCAAGGGGCTG8970.10281497545392754No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA8940.10247111265976724No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC8850.10143952427728636No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG8830.10121028241451284No Hit
CGTCCATACCGTCGTAGTAGTAGTAGTCTCCCGAACCATAATAATCTCGA8800.10086641962035255No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT8730.10006407310064522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA402.7818506E-4118.0901770
AATGGTA1952.746674E-1064.5963470
CTGTTTA1306.690212E-660.55906370
ATATGAG200.007702127651.15589529
CGATTCA13500.046.65291270
TAGGCAA1703.2737647E-546.3098770
CGTCACA6250.045.34662670
GAGCCAT12200.045.17110470
GTATCAA13650.042.904411
GTGGTAT1350.042.8766981
TAGAAAT11500.042.44400470
ATGTCCA16100.041.07484470
TATCAAC15500.037.7445832
AAGCGGT8450.037.26711770
ATGACTT1700.00182639537.04789770
CTCTTAA4751.4551915E-1136.46293370
ATCAACG16550.035.3478853
TCAACGC16650.035.139614
CAACGCA16700.035.0344055
AACGCAG17100.034.6127366
Produced by FastQC (version 0.11.5)