FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146077
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGAGGCTGGCCTAATTTTTGCTGGTACCAAGCTAAGGCGTTCTTATTGGTGGACCTATCTAAAAGAC83650.7298811510919424No Hit
CTCCTAGACTGCTCATTTATTGGGCGTCTACCCGGGAATCCGGGGTCCCAGACCGATTCAGTGGCAGCGGGTC83070.7248204090999122No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT53950.4707362594310854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52020.4538962041817435No Hit
GTCCCAGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGACTGAAGA43530.37981741191909446No Hit
CTTTTAGATAGGTCCACCAATAAGAACGCCTTAGCTTGGTACCAGCAAAA42100.3673400652835717No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT37910.3307805670997673No Hit
GGATTATAATATTGCTGACAGTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAA37700.32894822948196323No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33640.29352303553775183No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32940.287415243478405No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32810.2862809392388121No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32640.2847976183101135No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30800.2687428506112591No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29480.2572252998707766No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29180.2546076747024851No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28830.25155377867281165No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC28660.2500704577441132No Hit
TTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCT27450.23951270289867083No Hit
GTCTGGGACCCCGGATTCCCGGGTAGACGCCCAATAAATGAGCAGTCTAGGAGGCTGGCCTAATTTTTGCTGGTA27310.2382911444868015No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT27110.23654606104127385No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC26460.23087453984330897No Hit
ATCTAAAAGACTCTGGCTGGACCTGCAGTTGACGGTGGTTCTCTCGCCCAGAGACACAGCCAGGGAGCCTGGAGA25240.22022953082559024No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTACTTACGGTGACATCGTGATGACCCAG24510.2138599762494143No Hit
ATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCT24090.21019530101380623No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24000.20941001346331875No Hit
ATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCA21930.19134839980210755No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGACTGAAGATGTGGCGATTTATTACTGTCAGCAATATTATAATCC21440.1870729453605648No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19900.17363580283000182No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA19540.17049465262805205No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19370.16901133169935353No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19310.16848780666569524No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAATGTC19300.16840055249341884No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19280.16822604414886608No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGC18900.16491038560236354No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18820.16421235222415248No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18520.161594727055861No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18130.15819181433708207No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTACTTACGG17740.15478890161830314No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17640.15391635989553928No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17410.1519095139331825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17190.14998992214310208No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16660.1453654510124538No Hit
GTCTCCAGGCTCCCTGGCTGTGTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGCCAGAGTCTTTTAGA16510.14405663842830804No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT16360.1427478258441623No Hit
GGACCAAGGTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC16110.14056647153725274No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16070.1402174548481472No Hit
GTCTGGGGCTGACGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA16040.13995569233131805No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACCCCGGATTCCCGGGT15990.13951942146993612No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG15760.13751257550757934No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15500.1352439670283934No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACCCCGGATTCCCGGGTAGACGCCCAATAAA15300.1334988835828657No Hit
CACCATCAACAGCCTGCAGACTGAAGATGTGGCGATTTATTACTGTCAGCAATATTATAATCCTCCGTGGACAT14990.13079400424229787No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14490.1264312956284787No Hit
TTATAATATTGCTGACAGTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC14380.12547149973343852No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14380.12547149973343852No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14330.1250352288720566No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14280.12459895801067468No Hit
GCTCTGGATCTCTGGTACTTACGGTGACATCGTGATGACCCAGTCTCCAG14210.12398817880473999No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14090.1229411287374234No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13940.12163231615327766No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG13840.12075977443051383No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13810.12049801191368469No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13800.1204107577414083No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13770.12014899522457916No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC13640.11901469098498617No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT13580.11849116595132787No Hit
CAGTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13550.11822940343449873No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13540.11814214926222234No Hit
CTGCAGGTCCAGCCAGAGTCTTTTAGATAGGTCCACCAATAAGAACGCCTTAGCTTGGTACCAGCAAAAATTAGG13530.11805489508994596No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13460.11744411588401127No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13410.11700784502262937No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCT13320.11622255747214194No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13320.11622255747214194No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT13270.11578628661076001No Hit
GAATCGGTCTGGGACCCCGGATTCCCGGGTAGACGCCCAATAAATGAGCAGTCTAGGAGGCTGGCCTAATTTT13270.11578628661076001No Hit
GTAATAAATCGCCACATCTTCAGTCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13260.11569903243848363No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13230.11543726992165448No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13100.11430296568206151No Hit
CCCCTGGACAAGGGCTTCAGTGGATGGGAATAATCAACCCTGCTAGTGGT13020.11360493230385045No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13010.11351767813157405No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12890.11247062806425745No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG12810.11177259468604639No Hit
CCTTAGCTTGGTACCAGCAAAAATTAGGCCAGCCTCCTAGACTGCTCATT12770.11142357799694086No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12740.11116181548011173No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12700.1108127987910062No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12670.11055103627417703No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCTGGGACCCCGGATTCCCGGGTAGA12600.10994025706824236No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAA12600.10994025706824236No Hit
GCCTCCTAGACTGCTCATTTATTGGGCGTCTACCCGGGAATCCGGGGTCCCAGACCGATTCAGTGGCAGCGGG12510.10915496951775493No Hit
ATTTATTACTGTCAGCAATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA12470.1088059528286494No Hit
CCATCAACAGCCTGCAGACTGAAGATGTGGCGATTTATTACTGTCAGCAA12380.10802066527816194No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12350.10775890276133279No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12300.10732263189995087No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12300.10732263189995087No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12290.1072353777276745No Hit
CCTTGGTCCCTTGGCCGAATGTCCACGGAGGATTATAATATTGCTGACAGTAATAAATCGCCACATCTTCAG12240.10679910686629257No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGTACTTACGGTGACATCGTGA12090.10549029428214685No Hit
CTGAAGATGTGGCGATTTATTACTGTCAGCAATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGG12030.10496676924848854No Hit
GTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGCCAGAGTCTTTTAGATAGGTCCACCAATAAGAACGC11960.10435599004255385No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11750.10252365242474981No Hit
GGCTGTGTCTCTGGGCGAGAGAACCACCGTCAACTGCAGGTCCAGCCAGA11630.10147660235743322No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11570.10095307732377493No Hit
TTATTACTGTCAGCAATATTATAATCCTCCGTGGACATTCGGCCAAGGGACCAAGGTGGAAATGAAACGAACTG11550.10077856897922216No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11540.10069131480694579No Hit
GTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGACTGAAGATGTGGCGATTTATTACTGTCAGCAATA11510.10042955229011663No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11510.10042955229011663No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT11510.10042955229011663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)